LOCUS       sequence-176990-        5757 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  For transcription of of Suv4-20h1mut mRNA preceded by an HA-tag in 
            5'.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Eid A, Rodriguez-Terrones D, Burton A, Torres-Padilla ME
  TITLE     SUV4-20 activity in the preimplantation mouse embryo controls timely
            replication.
  JOURNAL   Genes Dev. 2016 Nov 15;30(22):2513-2526. Epub 2016 Dec 5.
  PUBMED    27920088
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2016-11-15"; volume: "30(22)"; pages: "2513-2526. Epub
            2016 Dec 5"
COMMENT     Sequence Label: pRN3P-HA-Suv4-20h1mut
FEATURES             Location/Qualifiers
     source          1..5757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2776..2802)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      2805..2814
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     5'UTR           complement(2822..2864)
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     promoter        complement(2889..2907)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2928..2944)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2928..2944)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2941..2963)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2952..2968
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2976..2982,2983..3000,3001..3006))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3021..3042
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3159..3176)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3330..3918)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3410..3429)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4089..4880,4881..4949))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4712..4731
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4950..5054)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5081..5536
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5168..5187)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5378..5399
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5663..5685
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5677..5694
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5678..5694
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5701..5720
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5701..5719
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agaaactttc tttttattag gagcagatac gaatggctac attttggggg acaacatttt
       61 gtaaagtgta agttggtatt atgtagctta gagactccat tcgggtgttc ttgaggctgg
      121 tttagtggta accagatccg cggccgctgc gttcagtctt agagactgat cttctcttct
      181 ccttgaagaa atgtcaatat ctatggagtc tttaccaaca attagcctca gccgctttgc
      241 cggaggaaga ggaatgaagt catcatcgaa gtcatcctcg cagtcctcct cctcctcctc
      301 cccctcagaa gatgaggatt catctgtgct gtcctcctca tattgactgt agtcgtccac
      361 ctccatctgg gactcgagca gggagagggg gtcgctgcag cacaccccgt tctcgtgcag
      421 gccctctgca catggccccc tgctctcctc atcccgcttg agctggatct tgagcttggt
      481 ggagctgctg cccggccctg cgctgacccc gttactgagc ttggcaacat agaggttgct
      541 gtcactgtcg tggtccttgc tgagtttgat gctgatcttg gaggagttca cgccgtcact
      601 ctcatggtcg tttgtcttgc tgctgctgtc gtgccgcctg cgaagcgtca gcttggggat
      661 tccagagctg ttctccagga tcagctgtgc atcgtacctg gtgacccgcc gcttcttttt
      721 actttgtgca gttctgaagc tgtcttttgt gaagctgtcg ggtgcgacaa cagagcagcc
      781 aaccggtgag ggagcagagt ccgtgtggct cacgggcacc ctgactgtgc cactgggctc
      841 accttgatca ggatgagacc ctggcaaatc tggcagcccg tctttcccag ggaagctgtg
      901 ctctggactg cagtcctcgg tctttgctaa gtgtttcaca ggtttcactt gtcgaaattt
      961 ctttcgtgac aggcaggagt cgttcttggg acactcctgg gatgcttcct ccctgtgtgc
     1021 agtctcagga gccagttctt ccagcacctc tggggttggc tgctggccac tgtctgggca
     1081 aggttccagt ataccatttt tatagccatc caagggactt ggttcaagct tgatgtcagt
     1141 ggtctccttc agacctgtcc ttgtcctcaa agagcgccgg gttgtgtagg tgcagggcaa
     1201 actgtcgccc tgcgaatgag cacctctccc atgattctgc ctgtgttctc tcgcagcatg
     1261 tctagtcaag cacccactcc ccactgcagc atgggctggt ccctctggct ccctttcttt
     1321 cttaattggc aaatttttat atagcacgac tttgggctcc ttaagcacta agctccccat
     1381 ctcgagcttg cgggatgcgc tcttctggtc cagccgcttg cagtgattcc gcagtctgat
     1441 cttggagagt aaaggctttg ccggctttct cagtttcttt ggtacccggg gattgttggt
     1501 gtgcactagc ttgggtgagg tagagttgga agcagccggg actctcggca tggacggcct
     1561 tgtcagagct cgactcttgc tgctcttttt cacaccaact gaagattttc gattagaagt
     1621 tgcattgtct ttttcctgag tggtgtctgc atctgtgtta gagctgacag actgactgtc
     1681 tgagtttttg ctgctgtcac ctaacttttt aagcctattt aagcgtttat ctgtttctct
     1741 aagtccgtat ttgctattga taacaggagc aggcgcaggc agtcctactc gtgatttaaa
     1801 agcaccagtt ccccgtcttt cacaagtata acattcgcag aactcattat tttctccaaa
     1861 aaagccatct ccatagtaac aagaaatttc ttctccaggt tcaatatctc tcagagcctt
     1921 aacgcatgct gtatctcgac cagttgacac aaacttacag ttaggtctgc ctgctgcagc
     1981 tataaatgca gcaggaccga gccagagttg agcacaattt ttccttgtgg aatacatgac
     2041 actgaagtcg ttttctccgt gtctaagtag catgttctcc tcaatttctg aaagttcggc
     2101 aatacaaccc accagtaatt ctattttgtc atttcgtttc cactcttttg ttgcaactat
     2161 cttggctcca ttttgttctg aagaatatct attacaaggc agtatttcaa atccactgtc
     2221 agttgcaaac atccgcaagt aaataaagac atgttccttg aataatttct cctgcatttt
     2281 gtttttgttg agaaaataat gccgtgccca ttccccagaa gtcaaacatt tgaaagcttt
     2341 ctctaagtgt tcatctttct taaagcgttc aattacttcc tttagctctt cttgccttcc
     2401 ttttataggc cgaaatctca caggattgtt gtgagaaaaa ctgtcagctt ttgaaatatg
     2461 cctcgagctc ctcgaaggaa aggcgctagt gttcattttg tgtgtctgaa aacctaagta
     2521 gggatcaaga accaaactgg ttgctaagtc atcgttctca cagagctcct tggcggacat
     2581 tccagaggac ggcacatagc ggctctgccc ttcaaatccc gagttaccat gacatctgct
     2641 ggaccgcctc tcaacggcgt ttctgccggt cttcagggtg tccttgcccg agtgctgctg
     2701 taattttgat tgattctgct gatggtcatt agacaacttg cctccatttc tcctgccatt
     2761 caccaccatg gatcccgcat agtcaggaac atcgtatggg tagcccatgg cggcagatct
     2821 gccaaagttg agcgtttatt ctgagcttct gcaaaaagaa caagcaagct tagctccagc
     2881 ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt
     2941 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     3001 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     3061 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     3121 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     3181 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     3241 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     3301 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     3361 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     3421 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     3481 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     3541 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     3601 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     3661 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     3721 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     3781 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     3841 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     3901 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     3961 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     4021 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     4081 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     4141 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     4201 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     4261 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     4321 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     4381 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     4441 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     4501 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     4561 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     4621 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     4681 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     4741 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     4801 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     4861 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     4921 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     4981 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     5041 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca
     5101 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5161 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5221 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5281 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5341 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5401 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5461 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5521 ttaacgctta caatttgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     5581 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     5641 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg
     5701 taatacgact cactataggg cgaattgggt accggccgac ttggccctgc agggggg
//
