LOCUS       sequence-17729-e         940 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Meister G, Landthaler M, Patkaniowska A, Dorsett Y, Teng G, Tuschl T
  TITLE     Human Argonaute2 mediates RNA cleavage targeted by miRNAs and 
            siRNAs.
  JOURNAL   Mol Cell. 2004 Jul 23. 15(2):185-97.
  PUBMED    15260970
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2004 Jul 23. 15(2):185-97."
COMMENT     Sequence Label: pIRESneo-FLAG/HA Ago4
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      112..121
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             121..144
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             145..171
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttggtacc gagctcggat cgatatcgcc ggccgccatg
      121 gactacaagg acgacgatga caagtaccct tatgacgtgc ccgattacgc tagcggccgc
      181 atggaggcgc tgggacccgg acctccggct agcctgtttc agccacctcg tcgtcctggc
      241 cttggaactg ttggaaaacc aattcgactg ttagccaatc attttcaggt tcagattcct
      301 aaaatagatg tgtatcacta tgatgtggat attaagcctg aaaaacggcc tcgtagagtc
      361 aacagggagg tagtagatac aatggtgcgg cacttcaaga tgcaaatatt tggtgatcgg
      421 cagcctgggt atgatggcaa aagaaacatg tacacagcac atccactacc aattggacgg
      481 gatagggttg atatggaggt gactcttcca ggcgagggta aagaccaaac atttaaagtg
      541 tctgttcagt gggtgtcagt tgtgagcctt cagttgcttt tagaagcttt ggctgggcac
      601 ttgaatgaag tcccagatga ctcagtacaa gcacttgatg ttatcacaag acaccttccc
      661 tccatgaggt acaccccagt gggccgttcc tttttctcac ccccggaagg ttactaccac
      721 cctctgggag ggggcaggga ggtctggttt ggttttcatc agtctgtgag acctgccatg
      781 tggaatatga tgctcaacat tgatgtatct gcaactgctt tctaccgggc tcagcctatc
      841 attgagttca tgtgtgaggt tttagacatt cagaacatca atgaacagac caaacctcta
      901 acagactccc agcgtgtcaa atttaccaaa gaaatcagag
//
