LOCUS       sequence-177463-        1076 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-HPCHISNDHFR
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(551..571)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(599..616)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             675..697
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(764..783)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(765..783)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggctttgtta gcagccggat cagcgcgcac gcgtggtacc aagcttagat ctggatcttg
       61 tacattagcc ggcacgacgc tcgaggattt cgaaacaata gctatgcgag ttctgcgcat
      121 cagcatcgtg gaattcgctg aataccgatt cccagtcatc cggctcgtaa tccggaaaat
      181 gggtgtcgcc ttccacttct gcatcgatat gcgtcagata cagtttttgc gcttttggca
      241 agaactgttc ataaacgcgt ccgccgccaa tcaccatgat ttctggtacg tcaccacacg
      301 ccgcgatggc ttcgtcgacc gatttaaccc aggtaacccg atcatcggtc ccgggttgac
      361 tgctgaggat aatatttttg cggccgggca aaggcctacc gattgattcc caggtatggc
      421 gccccataat cacgggttta tttaaggtgt tgcgtttaaa ccaggcgaga tcggcaggca
      481 ggttccatgg catggcgttt tccatgccga taacgcgatc taccgctagc gccgcaatca
      541 gactgatgga tccctggaag tacaggtttt caccaccacc accaccagat ccgctgctgt
      601 gatgatgatg atgatggcta gatcctttac cgtcgattaa acgtggatct acctgatctt
      661 cagacatatg tatatctcct tcttaaagtt aaacaaaatt atttctagag ctagcgaatt
      721 ccaattggag ctctccggag ccggagggaa accgttgtgg tctccctata gtgagtcgta
      781 ttaatttcgc gggatcgatg catggtgcca acacctacat ctgtattaac gaagcgctgg
      841 cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag ttgtttaccc
      901 tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc
      961 tctcgtttca tcggtatcat taccccatga acagaaatcc cccttacacg gaggcatcag
     1021 tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagaca
//
