LOCUS       sequence-177486-        6006 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Localization by Super-Resolution microscopy of alpha-Tubulin fused 
            to mEOS3.1 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6006)
  TITLE     Unpublished Henriques Super-Resolution Microscopy Plasmids
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RHPMP125 (mEOS3.1-alpha Tubulin)
FEATURES             Location/Qualifiers
     source          1..6006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             610..1287
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.1
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    2794..2915
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2831..2850)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2885..2904
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2922..3377)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3059..3080)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3271..3290
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3404..3508
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3510..3867
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3510..3701
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3533..3553)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3718..3853
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3902..4696
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3902..4696
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3956..3975)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4566..4585
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4884..4903)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4928..4975
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5304..5892
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5793..5812
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcggtc
      601 gccgccacca tgagtgcgat taagccagac atgaagatca aactccgtat ggaaggcaac
      661 gtaaacgggc accactttgt gatcgacgga gatggtacag gcaagccttt tgagggaaaa
      721 cagagtatgg atcttgaagt caaagagggc ggacctctgc cttttgcctt tgatatcctg
      781 accactgcat tccattacgg caacagggta ttcgccaaat atccagacaa catacaagac
      841 tattttaagc agtcgtttcc taaggggtat tcgtgggaac gaagcttgac tttcgaagac
      901 gggggcattt gcaacgccag aaacgacata acaatggaag gggacacttt ctataataaa
      961 gttcgatttt atggtaccaa ctttcccgcc aatggtccag ttatgcagaa gaagacgctg
     1021 aaatgggagc cctccactga gaaaatgtat gtgcgtgatg gagtgctgac gggtgatgta
     1081 gagatggctt tgttgcttga aggaaatgcc cattaccgat gtgacttcag aactacttac
     1141 aaagctaagg agaagggtgt caagttacca ggcgcccact ttgtggacca ctgcattgag
     1201 attttaagcc atgacaaaga ttacaacaag gttaagctgt atgagcatgc tgttgctcat
     1261 tctggattgc ctgacaatgc cagacgatcc ggactagatc tcgatcgagt gcgtgagtgc
     1321 atctccatcc acgttggcca ggctggtgtc cagattggca atgcctgctg ggagctctac
     1381 tgcctggaac acggcatcca gcccgatggc cagatgccaa gtgacaagac cattggggga
     1441 ggagatgact ccttcaacac cttcttcagt gagacgggcg ctggcaagca cgtgccccgg
     1501 gctgtgtttg tagacttgga acccacagtc attgatgaag ttcgcactgg cacctaccgc
     1561 cagctcttcc accctgagca gctcatcaca ggcaaggaag atgctgccaa taactatgcc
     1621 cgagggcact acaccattgg caaggagatc attgaccttg tgttggaccg aattcgcaag
     1681 ctggctgacc agtgcaccgg tcttcagggc ttcttggttt tccacagctt tggtggggga
     1741 actggttctg ggttcacctc cctgctcatg gaacgtctct cagttgatta tggcaagaag
     1801 tccaagctgg agttctccat ttacccagca ccccaggttt ccacagctgt agttgagccc
     1861 tacaactcca tcctcaccac ccacaccacc ctggagcact ctgattgtgc cttcatggta
     1921 gacaatgagg ccatctatga catctgtcgt agaaacctcg atatcgagcg cccaacctac
     1981 actaacctta accgccttat tagccagatt gtgtcctcca tcactgcttc cctgagattt
     2041 gatggagccc tgaatgttga cctgacagaa ttccagacca acctggtgcc ctacccccgc
     2101 atccacttcc ctctggccac atatgcccct gtcatctctg ctgagaaagc ctaccatgaa
     2161 cagctttctg tagcagagat caccaatgct tgctttgagc cagccaacca gatggtgaaa
     2221 tgtgaccctc gccatggtaa atacatggct tgctgcctgt tgtaccgtgg tgacgtggtt
     2281 cccaaagatg tcaatgctgc cattgccacc atcaaaacca agcgcagcat ccagtttgtg
     2341 gattggtgcc ccactggctt caaggttggc atcaactacc agcctcccac tgtggtgcct
     2401 ggtggagacc tggccaaggt acagagagct gtgtgcatgc tgagcaacac cacagccatt
     2461 gctgaggcct gggctcgcct ggaccacaag tttgacctga tgtatgccaa gcgtgccttt
     2521 gttcactggt acgtgggtga ggggatggag gaaggcgagt tttcagaggc ccgtgaagat
     2581 atggctgccc ttgagaagga ttatgaggag gttggtgtgg attctgttga aggagagggt
     2641 gaggaagaag gagaggaata ctaaggatcc accggatcta gataactgat cataatcagc
     2701 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     2761 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     2821 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     2881 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat
     2941 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     3001 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     3061 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     3121 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     3181 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     3241 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     3301 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     3361 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3421 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3481 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     3541 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3601 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     3661 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     3721 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     3781 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     3841 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     3901 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     3961 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4021 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     4081 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     4141 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     4201 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     4261 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     4321 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     4381 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     4441 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     4501 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     4561 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     4621 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     4681 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     4741 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     4801 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     4861 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     4921 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     4981 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     5041 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     5101 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     5161 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5221 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5281 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5341 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5401 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5461 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5521 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5581 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5641 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5701 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5761 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5821 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5881 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5941 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6001 atgcat
//
