LOCUS       sequence-177497-         989 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Position 2 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc2-FLAGHISNDHFR
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(532..552)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(580..597)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(607..630)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             644..666
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(721..740)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(722..740)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttagcagccg gatcagcgcg cacgcgtggt accaagcttt gtacattagc cggcacgacg
       61 ctcgaggatt tcgaaacaat agctatgcga gttctgcgca tcagcatcgt ggaattcgct
      121 gaataccgat tcccagtcat ccggctcgta atccggaaaa tgggtgtcgc cttccacttc
      181 tgcatcgata tgcgtcagat acagtttttg cgcttttggc aagaactgtt cataaacgcg
      241 tccgccgcca atcaccatga tttctggtac gtcaccacac gccgcgatgg cttcgtcgac
      301 cgatttaacc caggtaaccc gatcatcggt cccgggttga ctgctgagga taatattttt
      361 gcggccgggc aaaggcctac cgattgattc ccaggtatgg cgccccataa tcacgggttt
      421 atttaaggtg ttgcgtttaa accaggcgag atcggcaggc aggttccatg gcatggcgtt
      481 ttccatgccg ataacgcgat ctaccgctag cgccgcaatc agactgatgg atccctggaa
      541 gtacaggttt tcaccaccac caccaccaga tccgctgctg tgatgatgat gatgatggct
      601 agatcccttg tcatcatcgt ccttgtagtc tcccatatgt atatctcctt cttaaagtta
      661 aacaaaatta ttgaattcca attggagctc tccggagccg gagggaaacc gttgtggtct
      721 ccctatagtg agtcgtatta atttcgcggg atcgatgcat ggtgccaaca cctacatctg
      781 tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata
      841 ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc
      901 gtatcgtgag catcctctct cgtttcatcg gtatcattac cccatgaaca gaaatccccc
      961 ttacacggag gcatcagtga ccaaacagg
//
