LOCUS       sequence-177622-       10252 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Cloning vector for co-expressing a gene with 4CL5 in S.cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10252)
  AUTHORS   Eudes A, Mouille M, Robinson DS, Benites VT, Wang G, Roux L, Tsai 
            YL, Baidoo EE, Chiu TY, Heazlewood JL, Scheller HV, Mukhopadhyay A, 
            Keasling JD, Deutsch S, Loque D
  TITLE     Exploiting members of the BAHD acyltransferase family to synthesize 
            multiple hydroxycinnamate and benzoate conjugates in yeast.
  JOURNAL   Microb Cell Fact. 2016 Nov 21;15(1):198.
  PUBMED    27871334
REFERENCE   2  (bases 1 to 10252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2016-11-21"; volume: "15(1)"; pages: "198"
COMMENT     Sequence Label: pDRf1-4CL5-DsRed (JBEI-15461)
FEATURES             Location/Qualifiers
     source          1..10252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      557..1012
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(644..663)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     854..875
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1042..1058)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1042..1058)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1055..1077)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1066..1082
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1090..1096,1097..1114,1115..1120))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1135..1156
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1273..1290)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1444..2032)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1524..1543)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2203..2994,2995..3063))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2826..2845
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3064..3168)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3236..3254
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3279..3494
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             3495..4298
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      4409..5755
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     primer_bind     6176..6198
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6190..6207
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6191..6207
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(6220..6467)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     6433..6451
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      complement(8620..8807)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     8728..8752
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     8955..8974
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8955..8973
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    9036..9060
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      complement(9075..9102)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(9118..9189)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(9207..9884)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             9891..9902
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    9920..9938
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9945..9963
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(9977..10001)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgataggaca tttgaaaata atacaatatt tggccttttt atataatcga aactaatgga
       61 ggggagcata agcggtaccc accatcgacc acgtttcgtc accggtcata attggaataa
      121 acaaaacaaa caaacgaact aaagagtgtg agagtaacaa tttccacaga ggtaaaaaaa
      181 aaaaaaagga aaacagaaac aaaaaaagct catagttcta ctatagcttc ttcagacttt
      241 ctgtgaacga gggaaaaaga caacaaaaaa gcaatggata aacggtctcg atcgaggcct
      301 ggaagtgccg cattaggaaa tacagatgca atgcgcgcga gtttacgaaa ttctcgcagg
      361 agtttctccg gccatgtgcg gctggttcta cgtctcgtgt atgggtttat ttctgcggta
      421 attatcgccg gatctccgct tcgagaccgg gtttgttttc aaattaccgg aaaaggtcag
      481 gcaaaacaga gtgatgtatg ggtgatccca gcaatgcctg tttatgtttc tccgtttcag
      541 gaagcttggc gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
      601 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
      661 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
      721 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
      781 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
      841 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
      901 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
      961 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc
     1021 ggtaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     1081 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     1141 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     1201 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1261 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1321 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1381 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1441 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1501 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1561 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1621 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1681 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1741 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1801 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1861 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1921 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1981 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2041 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2101 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2161 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2221 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2281 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2341 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2401 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2461 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2521 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2581 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2641 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2701 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2761 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     2821 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     2881 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     2941 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3001 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3061 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     3121 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     3181 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     3241 gcgtatcacg aggccctttc gtcttcaaga attagctttt caattcatca tttttttttt
     3301 attctttttt ttgatttcgg tttccttgaa atttttttga ttcggtaatc tccgaacaga
     3361 aggaagaacg aaggaaggag cacagactta gattggtata tatacgcata tgtagtgttg
     3421 aagaaacatg aaattgccca gtattcttaa cccaactgca cagaacaaaa acatgcagga
     3481 aacgaagata aatcatgtcg aaagctacat ataaggaacg tgctgctact catcctagtc
     3541 ctgttgctgc caagctattt aatatcatgc acgaaaagca aacaaacttg tgtgcttcat
     3601 tggatgttcg taccaccaag gaattactgg agttagttga agcattaggt cccaaaattt
     3661 gtttactaaa aacacatgtg gatatcttga ctgatttttc catggagggc acagttaagc
     3721 cgctaaaggc attatccgcc aagtacaatt ttttactctt cgaagacaga aaatttgctg
     3781 acattggtaa tacagtcaaa ttgcagtact ctgcgggtgt atacagaata gcagaatggg
     3841 cagacattac gaatgcacac ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg
     3901 cggcagaaga agtaacaaag gaacctagag gccttttgat gttagcagaa ttgtcatgca
     3961 agggctccct atctactgga gaatatacta agggtactgt tgacattgcg aagagcgaca
     4021 aagattttgt tatcggcttt attgctcaaa gagacatggg tggaagagat gaaggttacg
     4081 attggttgat tatgacaccc ggtgtgggtt tagatgacaa gggagacgca ttgggtcaac
     4141 agtatagaac cgtggatgat gtggtctcta caggatctga cattattatt gttggaagag
     4201 gactatttgc aaagggaagg gatgctaagg tagagggtga acgttacaga aaagcaggct
     4261 gggaagcata tttgagaaga tgcggccagc aaaactaaaa aactgtatta taagtaaatg
     4321 catgtatact aaactcacaa attagagctt caatttaatt atatcagtta ttacccaatt
     4381 ctcatgtttg acagcttatc atcggatcga tccaatatca aaggaaatga tagcattgaa
     4441 ggatgagact aatccaattg aggagtggca gcatatagaa cagctaaagg gtagtgctga
     4501 aggaagcata cgataccccg catggaatgg gataatatca caggaggtac tagactacct
     4561 ttcatcctac ataaatagac gcatataagt acgcatttaa gcataaacac gcactatgcc
     4621 gttcttctca tgtatatata tatacaggca acacgcagat ataggtgcga cgtgaacagt
     4681 gagctgtatg tgcgcagctc gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt
     4741 gaagttccta ttccgaagtt cctattctct agctagaaag tataggaact tcagagcgct
     4801 tttgaaaacc aaaagcgctc tgaagacgca ctttcaaaaa accaaaaacg caccggactg
     4861 taacgagcta ctaaaatatt gcgaataccg cttccacaaa cattgctcaa aagtatctct
     4921 ttgctatata tctctgtgct atatccctat ataacctacc catccacctt tcgctccttg
     4981 aacttgcatc taaactcgac ctctacattt tttatgttta tctctagtat tactctttag
     5041 acaaaaaaat tgtagtaaga actattcata gagtgaatcg aaaacaatac gaaaatgtaa
     5101 acatttccta tacgtagtat atagagacaa aatagaagaa accgttcata attttctgac
     5161 caatgaagaa tcatcaacgc tatcactttc tgttcacaaa gtatgcgcaa tccacatcgg
     5221 tatagaatat aatcggggat gcctttatct tgaaaaaatg cacccgcagc ttcgctagta
     5281 atcagtaaac gcgggaagtg gagtcaggct ttttttatgg aagagaaaat agacaccaaa
     5341 gtagccttct tctaacctta acggacctac agtgcaaaaa gttatcaaga gactgcatta
     5401 tagagcgcac aaaggagaaa aaaagtaatc taagatgctt tgttagaaaa atagcgctct
     5461 cgggatgcat ttttgtagaa caaaaaagaa gtatagattc tttgttggta aaatagcgct
     5521 ctcgcgttgc atttctgttc tgtaaaaatg cagctcagat tctttgtttg aaaaattagc
     5581 gctctcgcgt tgcatttttg ttttacaaaa atgaagcaca gattcttcgt tggtaaaata
     5641 gcgctttcgc gttgcatttc tgttctgtaa aaatgcagct cagattcttt gtttgaaaaa
     5701 ttagcgctct cgcgttgcat ttttgttcta caaaatgaag cacagatgct tcgttaacaa
     5761 agatatgcta ttgaagtgca agatggaaac gcagaaaatg aaccggggat gcgacgtgca
     5821 agattaccta tgcaatagat gcaatagttt ctccaggaac cgaaatacat acattgtctt
     5881 ccgtaaagcg ctagactata tattattata caggttcaaa tatactatct gtttcaggga
     5941 aaactcccag gttcggatgt tcaaaattca atgatgggta acaagtacga tccgattatg
     6001 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
     6061 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     6121 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     6181 tcacgacgtt gtaaaacgac ggccagtgaa ttggcatgcg caaattaaag ccttcgagcg
     6241 tcccaaaacc ttctcaagca aggttttcag tataatgtta catgcgtaca cgcgtctgta
     6301 cagaaaaaaa agaaaaattt gaaatataaa taacgttctt aatactaaca taactataaa
     6361 aaaataaata gggacctaga cttcaggttg tctaactcct tccttttcgg ttagagcgga
     6421 tgtgggggga gggcgtgaat gtaagcgtga cataactaat tacatgactc gagcggccgc
     6481 ctatttagag cacatggttt ccaatttagc tcgcagatcc ttcctcagaa tcttgcccga
     6541 aactgctttt ggtataactt ctatgaaaaa caccatctta atccgcttgt agtgaaccac
     6601 ctgtttgttt acgtaactct tgacatcatc ttcagttaac tgagaccctt gagatcttgc
     6661 tacaaatgct actggaacct catcagctac ttcatccttc attgcaacaa ctgcggcatc
     6721 atcgatagaa ggatgagaaa taagcaatgc ttcaagctca gctggagcca cttggtagcc
     6781 tttgaatttg atcagttcct tcaaccgatc aacaatgaag atctcatcat catcatccac
     6841 aaacccaata tctcctgtat gtaaccaccc gtctttgtct atggttcgtg cagtagcttc
     6901 cggatcattc aaataacctt tcatgagctg atgacctcgg acgcagatct cgccagattt
     6961 gttgcgcggc aaggagattc cggtttcggt atcgaccact ttcatctcgg cgtttctgat
     7021 cacagtccca catgcaccgg acttggtttt aaatgggttc tttgcaaacg ctaatgactt
     7081 agctaccgtt ccggactcgg tcattccata accctgacca aaaatggcat taggaaactt
     7141 gagtcgcacg gcgtcttcaa gctccttctt tagtgtagct gcacctgata gcataattct
     7201 caccgagctc aagtcgtatc tctccgtctc aggggattta atgaatgcta aaaccaccgg
     7261 aggagccacc ggcacaaccg tgactttgta cctttgaatc agctccatca ccagattcaa
     7321 ctcgaacctc ggcacgatca gtaacgctgc accggttctc atcgccgaaa gcatcaacgc
     7381 gtcgagagcg taaatgtgaa acattgggag aaaacagagg atgacgtcat tggccgtgaa
     7441 gttaaggtta gggttttctc cgtcgacttt ctgagcgatg cttgtaacta aacccttgtg
     7501 agtaatcatc actccctttg gaagtcccgt tgttccggaa gagtacggca tcgccaccgt
     7561 gtcttccggc gatattttag gttttagaag ttctgtttcg tccgcttgag tcagttccgt
     7621 gaaactcacg caaccatcat cactcgacga aacaacgccg ttgtcaccgt catcgtctag
     7681 gcaaacgatc aaaacgccgt cgtttttgag gtttgttagt ttatcgacta aacatttctt
     7741 cgtgatgatc atcttcgcgg cggaggcttt agcctgttta gcgatctccg gctgagtgta
     7801 gaacggattc gcggtagtgg aaacggctcc aaggtaagca acggcaagaa aagacagagc
     7861 aaactccggc gagttcggga ggagaagcat tacgacgtct ccgtggcgga tcccgagccg
     7921 gtggattccg gcagctatcc tccgcatgtt ggtttgtaca tcggcgtagg tcaaaatacg
     7981 gccagtggca ccgtcgatga tacaggttgt tgaggaatct ccgtcgccat cgccggagaa
     8041 cctttggaag acgtaatcgg tgagagggag gtggttaggg atgaagatat caggaagctt
     8101 agatcggaaa atgaaatcat gagaaggttc ttcttcttcg tcttcttgat ctatcttctt
     8161 tgtgagaaaa tgcgtttgtt gttggagcac catgcggccg cccgggggat ccactagaac
     8221 tttttgatta aaattaaaaa aactttttgt ttttgtgttt attctttgtt cttagaaaag
     8281 acaagttgag cttgtttgtt cttgatgttt tattatttta caatagctgc aaatgaagaa
     8341 tagattcgaa cattgtgaag tattggcata tatcgtctct atttatactt tttttttttc
     8401 agttctagta tattttgtat tttcctcctt ttcattcttt cagttgccaa taagttacag
     8461 gggatctcga aagatggtgg ggatttttcc ttgaaagacg actttttgcc atctaatttt
     8521 tccttgttgc ctctgaaaat tatccagcag aagcaaatgt aaaagatgaa cctcagaaga
     8581 acacgcaggg gcccgaaatt gttcctacga gctcgcatgc cggtagaggt gtggtcaata
     8641 agagcgacct catgctatac ctgagaaagc aacctgacct acaggaaaga gttactcaag
     8701 aataagaatt ttcgttttaa aacctaagag tcactttaaa atttgtatac acttattttt
     8761 tttataactt atttaataat aaaaatcata aatcataaga aattcgctta tttagaagtg
     8821 tcaacaacgt atctaccaac gatttgaccc ttttccatct tttcgtaaat ttctggcaag
     8881 gtagacaagc cgacaacctt gattggagac ttgaccaaac ctctggcgaa gaagtccaaa
     8941 gctggatccg cgcgtaatac gactcactat agggcgaatt gggtaccggg ccccccctcg
     9001 aggtcgacct gcagcggcca tcatgaatta actacaccac tttgtacaag aaagctgggt
     9061 ccacgtgcta taaacgcaga aaggcccacc cgaaggtgag ccagtgtgac tctagtagag
     9121 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg
     9181 ttttatttga tgcctggaga tccttattaa gcaccggtgg agtgacgacc ttcagcacgt
     9241 tcgtactgtt caacgatggt gtagtcttcg ttgtgggagg tgatgtccag tttgatgtcg
     9301 gttttgtaag cacccggcag ctgaaccggt tttttagcca tgtaggtggt tttaacttca
     9361 gcgtcgtagt gaccaccgtc tttcagtttc agacgcattt tgatttcacc tttcagagca
     9421 ccgtcttccg ggtacatacg ttcggtggaa gcttcccaac ccatggtttt tttctgcata
     9481 accggaccgt cggacgggaa gttggtacca cgcagtttaa ctttgtagat gaactcaccg
     9541 tcttgcaggg aggagtcctg ggtaacggta acaacaccac cgtcttcgaa gttcataaca
     9601 cgttcccatt tgaaaccttc cgggaaggac agtttcaggt agtccgggat gtcagccggg
     9661 tgtttaacgt aagctttgga accgtactgg aactgcgggg acaggatgtc ccaagcgaac
     9721 ggcagcggac cacctttggt aactttcagt ttagcggtct gggtaccttc gtacggacga
     9781 ccttcacctt caccttcgat ttcgaactcg tgaccgttaa cggaaccttc catacgaact
     9841 ttgaaacgca tgaactcttt gataacgtct tcgctactcg ccatagatcc tttctcctct
     9901 ttcagatccg tgctcagtat ctctatcact gatagggatg tcaatctcta tcactgatag
     9961 ggaagatcca cgtggaagcc tgcttttttg tacaaacttg tgatggccgc gaattcacta
    10021 gtcgaggctg gggtatattt tttttctttc ttttgaatgt gtgtataaaa gagagaaaaa
    10081 atggttattt tccaattaat taattattaa cgattgttaa agaaaaaatt tatcaacgag
    10141 gttgatagaa aaaaaaagtt tttgtagata tagaagaaaa aaagattttc actattggtg
    10201 ttataggaaa gaaagagaaa aaatttaaga gaaattagat gttagacgat aa
//
