LOCUS       sequence-177687-       11881 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses wildtype mouse TET2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11881)
  AUTHORS   Nakagawa T, Lv L, Nakagawa M, Yu Y, Yu C, D'Alessio AC, Nakayama K, 
            Fan HY, Chen X, Xiong Y
  TITLE     CRL4(VprBP) E3 Ligase Promotes Monoubiquitylation and Chromatin 
            Binding of TET Dioxygenases.
  JOURNAL   Mol Cell. 2015 Jan 22;57(2):247-60. doi: 
            10.1016/j.molcel.2014.12.002. Epub 2014 Dec 31.
  PUBMED    25557551
REFERENCE   2  (bases 1 to 11881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.12.002"; journalName: "Mol Cell"; date: 
            "2015-01-22- 22"; volume: "57"; issue: "2"; pages: "247-60"
COMMENT     Sequence Label: pcDNA3-FLAG-mTET2
FEATURES             Location/Qualifiers
     source          1..11881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(73..93)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        78..407
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      258..393
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     320..339
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             474..1268
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(528..547)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1138..1157
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1442..1563
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1479..1498)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1533..1552
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1612..1628)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1612..1628)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1625..1647)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1636..1652
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1660..1666,1667..1684,1685..1690))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1705..1726
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1843..1860)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2014..2602)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2094..2113)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2773..3564,3565..3633))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3396..3415
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3634..3738)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3813..3832)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4004..4383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4384..4587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4538..4558
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4632..4651
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4632..4650
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          4687..5259
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             5305..5328
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(11203..11221)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(11204..11221)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(11241..11258)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11247..11471
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(11517..11881,1..64)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11604..11623)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11814..11835
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
       61 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
      121 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
      181 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
      241 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      301 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
      361 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
      421 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
      481 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
      541 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
      601 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
      661 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
      721 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
      781 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
      841 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
      901 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
      961 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     1021 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     1081 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     1141 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     1201 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     1261 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     1321 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     1381 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     1441 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     1501 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     1561 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     1621 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     1681 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     1741 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     1801 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     1861 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     1921 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1981 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2041 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2101 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2161 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2221 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2281 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2341 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2401 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2461 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2521 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2581 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2641 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2701 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2761 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2821 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2881 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     2941 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3001 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3061 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3121 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3181 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     3241 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     3301 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     3361 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3421 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     3481 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     3541 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     3601 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     3661 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     3721 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     3781 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     3841 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     3901 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     3961 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     4021 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     4081 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     4141 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     4201 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     4261 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     4321 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     4381 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     4441 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     4501 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     4561 cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg
     4621 cttatcgaaa ttaatacgac tcactatagg gagacccaag ctagagtcga tcctgagaac
     4681 ttcagggtga gtttggggac ccttgattgt tctttctttt tcgctattgt aaaattcatg
     4741 ttatatggag ggggcaaagt tttcagggtg ttgtttagaa tgggaagatg tcccttgtat
     4801 caccatggac cctcatgata attttgtttc tttcactttc tactctgttg acaaccattg
     4861 tctcctctta ttttcttttc attttctgta actttttcgt taaactttag cttgcatttg
     4921 taacgaattt ttaaattcac ttttgtttat ttgtcagatt gtaagtactt tctctaatca
     4981 cttttttttc aaggcaatca gggtatatta tattgtactt cagcacagtt ttagagaaca
     5041 attgttataa ttaaatgata aggtagaata tttctgcata taaattctgg ctggcgtgga
     5101 aatattctta ttggtagaaa caactacatc ctggtcatca tcctgccttt ctctttatgg
     5161 ttacaatgat atacactgtt tgagatgagg ataaaatact ctgagtccaa accgggcccc
     5221 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
     5281 tgtgctgtcg accccaagct tatggactac aaagacgatg acgacaaggg atccactagt
     5341 aacggccgcc agtgtgctgg aattcgccct ttagaacagg acagaaccac ccatgctgag
     5401 ggcaccagac tgagtccatt cctgatagca ccaccttctc ccatcagcca tacagaacct
     5461 ctggctgtca aactccagaa tggaagcccg ttagcagaga gacctcatcc agaagtaaat
     5521 ggagacacca agtggcaatc ttcccaaagc tgttatggaa tatcccacat gaaaggcagc
     5581 cagagcagtc atgagagtcc acatgaagac agagggtatt ccaggtgctt acagaatgga
     5641 gggataaaac gcacagtcag tgaaccatct ctctctgggc tccatccaaa caagatattg
     5701 aaactagacc aaaaagctaa gggagaaagc aatatcttcg aggaaagcca agaaagaaac
     5761 cacggtaaaa gcagccgtca gccaaatgtc tccggactaa gtgataatgg agagcccgtg
     5821 acctcgacca cccaggaaag ttcaggtgca gatgctttcc caacacggaa ctacaatgga
     5881 gttgaaattc aggttctcaa cgagcaggaa ggggaaaaag gcaggagcgt tacattactt
     5941 aaaaacaaga ttgtgctaat gcctaatggt gctacagttt ctgcccattc tgaggagaac
     6001 acacgtggtg aactcctgga aaaaacacag tgttatccag attgtgtctc cattgccgtg
     6061 cagagtaccg catctcatgt aaacacccct agcagtcagg ctgctatcga gttgtcccac
     6121 gagatccctc aaccatcgct tacctcagcg cagatcaatt tctcccagac ctcaagcttg
     6181 cagctgcctc cagagccagc tgcaatggtg actaaggcct gtgatgctga taatgccagt
     6241 aaaccagcta tagtaccagg tacctgtcct tttcagaaag cagaacacca acaaaagtca
     6301 gctttggaca taggcccatc tcgtgcagaa aacaaaacca tccaaggaag catggagcta
     6361 tttgctgaag aatactatcc tagttccgac cggaatttgc aagcttcgca tggcagctct
     6421 gaacagtatt caaagcaaaa ggaaaccaat ggtgcttact tcaggcaaag ctcgaagttc
     6481 cctaaagatt ccatctctcc cactactgtg accccaccgt cacaatcact tcttgctccc
     6541 cgtcttgttc ttcagcctcc tttagaagga aaaggcgctc taaatgatgt agctttggaa
     6601 gaacaccatg actaccccaa ccgaagcaac cgaactcttt taagggaagg gaaaatagac
     6661 catcaaccca agacatcatc tagccagagt ctgaatccat ctgtacatac acccaacccc
     6721 cccttgatgc ttccagaaca gcatcagaat gattgtggct caccgagccc tgaaaagtca
     6781 agaaaaatgt cagaatatct catgtattac ctgccaaatc atggccacag tggaggttta
     6841 caagaacata gccaatacct gatggggcac agggagcaag agattccgaa ggatgcaaac
     6901 gggaaacaaa cgcaaggctc tgtacaggca gcacctggct ggatagaact gaaagccccg
     6961 aatttgcatg aagcactcca tcagacaaaa cgcaaggata tatccttgca ctcagtcctc
     7021 cactctcaga ccggccctgt caatcagatg agctccaaac agtccactgg caatgtcaac
     7081 atgccaggag gattccaaag gctaccttac ctccagaaaa cagcccagcc agagcagaag
     7141 gcacaaatgt accaagtgca agtgaaccaa ggaccgtctc caggtatggg ggaccaacat
     7201 cttcagttcc agaaagcttt ataccaggag tgcatcccca ggacagatcc gtcatctgag
     7261 gctcacccgc aagcaccgag cgttcctcag tatcatttcc agcaaagagt aaatccctcc
     7321 agtgataagc atttgagtca acaggccaca gagactcaac ggttatcagg ctttttacaa
     7381 catactcctc agacgcaggc atcacaaaca ccagcatccc agaactcaaa tttccctcaa
     7441 atctgccagc agcagcagca gcagcagcag cagttacaga ggaagaataa agagcaaatg
     7501 cctcagactt tctctcatct ccaaggtagc aatgataagc aaagagaagg ctcgtgcttt
     7561 ggccagatta aagtggaaga aagcttttgt gtcggaaatc agtactccaa atcaagtaat
     7621 ttccaaactc acaataatac ccaagggggg ttggagcaag tacaaaatat aaataaaaat
     7681 tttccttatt cgaagatctt aacaccaaat tcgagcaact tacagattct cccttcaaat
     7741 gacacacacc cggcttgtga gcgggaacaa gctctacatc ccgtaggaag taagacctca
     7801 aacctgcaga acatgcagta tttcccgaat aatgtgaccc caaatcagga cgttcaccgg
     7861 tgctttcagg aacaagcgca gaagcctcag caagcttcgt ctctacaggg gcttaaggac
     7921 agaagccagg gtgagtctcc agccccacca gctgaggcag ctcaacagag gtatttggtg
     7981 cataatgaag caaaggcact ccctgtgcct gagcaaggag gaagtcagac acagacccct
     8041 cctcagaagg acactcagaa gcacgctgcc ttaaggtggc ttctcttaca gaagcaagaa
     8101 cagcagcaaa cacagcaatc ccagcctggt cataaccaga tgcttaggcc aatcaagact
     8161 gagcctgtat ccaaaccttc ttcctataga taccccttgt caccgccaca agaaaatatg
     8221 tccagcagga taaagcaaga gatctcctct ccaagccgtg acaatgggca gccaaagagc
     8281 atcattgaga ccatggaaca gcacctgaag cagtttcagc tcaagtcact ctgtgactat
     8341 aaggctctga ctctcaagtc acagaaacac gtgaaagtgc caacagatat ccaggctgca
     8401 gaatcggaga accacgcccg agctgcagag cctcaagcaa ccaaaagcac agattgttct
     8461 gttctcgacg atgtttcaga atcagatact cctggtgaac aaagtcagaa tggcaaatgt
     8521 gaaggatgca atccagacaa agatgaagct ccttattata cccatctagg agctggtcct
     8581 gatgtggcag ctattagaac actcatggaa gaaaggtatg gagagaaggg taaagctatt
     8641 aggattgaaa aagtcatata tactggtaaa gaaggcaaga gctctcaggg atgtcctatt
     8701 gctaaatggg tatatcggag atcgagtgag gaggagaaac tactgtgttt ggtacgagtg
     8761 cgacctaacc acacatgtga gacggcggtg atggtaattg ccatcatgtt gtgggacgga
     8821 atcccaaagc tactcgcatc agaactctac tcagaactta cagatatctt gggcaagtgt
     8881 ggcatatgca ccaaccgtcg ctgttctcag aatgaaacga agaaaaagca atcaccaccc
     8941 agaaactgtt gttgtcaggg tgagaatcca gagacctgtg gtgcctcctt ttcttttggt
     9001 tgttcttgga gcatgtacta taatggatgt aagtttgcca gaagcaagaa accaaggaaa
     9061 tttaggctac atggagctga gccaaaagag gaagagagac taggttctca tttgcaaaac
     9121 ctggctactg tcattgctcc aatatacaag aagcttgcac ccgatgcata caataatcag
     9181 gttgaatttg aacaccaagc cccagactgc tgtttgggtc tgaaggaagg ccggccattc
     9241 tcaggagtca ctgcatgttt ggacttctct gctcattccc acagagacca gcagaacatg
     9301 ccaaatggca gtacagtggt ggtcaccctc aatagagaag acaatcgaga agtcggagct
     9361 aagcctgagg atgagcagtt ccacgtgctg cctatgtaca tcatcgcccc tgaggatgag
     9421 tttgggagta cggaaggcca ggagaagaag atacggatgg ggtccattga ggttctgcag
     9481 tcatttcgga ggagaagggt cataaggata ggagagctgc ccaagagttg caagaagaaa
     9541 gcggagccca agaaagccaa gaccaagaaa gcagctcgaa agcattcctc tctggagaac
     9601 tgctccagta ggactgagaa gggaaagtct tcctcacata caaagctgat ggaaaatgca
     9661 agccatatga aacaaatgac agcacaaccg cagctttcgg gcccggtcat ccggcagcca
     9721 ccaacactcc agaggcacct tcagcaaggg cagaggccac agcagccgca gccacctcag
     9781 ccgcagccgc agacgacacc tcagccacag ccacagccac agcatatcat gcccggtaac
     9841 tctcagtctg ttggttctca ttgttctgga tccaccagtg tctacacgag acagcctact
     9901 cctcacagtc cttatcccag ctcagcacac acctcagata tttatggaga taccaaccat
     9961 gtgaactttt accccacttc atctcatgcc tcgggttcat atttgaatcc ttctaattac
    10021 atgaacccct accttgggct tttgaatcag aataaccaat atgcaccttt tccatacaat
    10081 gggagtgtgc cagtggacaa tggttcccct ttcttaggtt cttattcccc ccaggctcag
    10141 tccagggatc tacatagata tccaaaccag gaccatctca ccaatcagaa cttaccaccc
    10201 atccacaccc ttcaccaaca gacgtttggg gacagtccct ctaagtactt aagttatgga
    10261 aaccaaaata tgcagagaga tgccttcact actaactcca ccctaaaacc aaatgtacac
    10321 cacctagcaa cgttttctcc ttaccccacc cccaagatgg atagtcattt catgggagct
    10381 gcctccagat caccatacag ccacccacac actgactaca aaaccagtga gcatcatcta
    10441 ccctctcaca cggtctacag ctacacggca gcagcttcgg ggagcagttc cagccacgcc
    10501 ttccacaaca aggagaatga caacatagcc aatgggctct caagagtgct tccagggttt
    10561 aatcatgata gaactgcttc tgcccaagaa ctattataca gtctgactgg cagcagtcag
    10621 gagaagcagc ctgaggtgtc aggccaggat gcagctgctg tgcaggaaat tgagtattgg
    10681 tcagatagtg agcacaactt tcaggatcct tgcattggag gggtggctat agccccaact
    10741 catgggtcaa ttcttattga gtgtgcaaag tgtgaggttc atgccacaac caaagtaaac
    10801 gatcccgacc ggaatcaccc caccaggatc tcacttgtac tgtataggca taagaatttg
    10861 tttctaccaa aacattgttt ggctctctgg gaagccaaaa tggctgaaaa ggcccggaaa
    10921 gaggaagagt gcggaaagaa tggatcagac cacgtgtctc agaaaaatca tggcaaacag
    10981 gaaaagcgtg agcccacagg gccacaggaa cccagttacc tgcgtttcat ccagtctctt
    11041 gctgagaaca cagggtctgt gactacggat tctaccgtga ctacatcacc atatgctttc
    11101 actcaggtca cagggcctta caacacattt gtatgaaagg gcgaattctg cagatatcca
    11161 tcacactggc ggccgctcga gcatgcatct agagggccct attctatagt gtcacctaaa
    11221 tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
    11281 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
    11341 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
    11401 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
    11461 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
    11521 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
    11581 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
    11641 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
    11701 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
    11761 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
    11821 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
    11881 g
//
