LOCUS       sequence-177970-       10062 bp    DNA     circular SYN 14-APR-2025
DEFINITION  The vector can ef_ciently produce a circRNA (circmCherry) from a 
            split EGFP gene after Dox treatment..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10062)
  AUTHORS   Zhang Y, Xue W, Li X, Zhang J, Chen S, Zhang JL, Yang L, Chen LL
  TITLE     The Biogenesis of Nascent Circular RNAs.
  JOURNAL   Cell Rep. 2016 Apr 19;15(3):611-24. doi: 
            10.1016/j.celrep.2016.03.058. Epub 2016 Apr 7.
  PUBMED    27068474
REFERENCE   2  (bases 1 to 10062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.03.058"; journalName: "Cell Rep"; date: 
            "2016-04-19- 19"; volume: "15"; issue: "3"; pages: "611-24"
COMMENT     Sequence Label: Tet-on circRNA Expression Vector
FEATURES             Location/Qualifiers
     source          1..10062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2228..2541
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2235..2253
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2271..2289
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2306..2324
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2342..2360
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2378..2396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2413..2431
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2449..2467
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2514..2538
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2569..2578
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2620..2641)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     3941..3960
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4288..4855
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4305..4857
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4449..4466)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4676..4695
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(5895..5914)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6242..6263
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6452..7040
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6505..6525)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6923..6934)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7115..7348
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7467..7485)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7553..7657
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7658..7726,7727..8518)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7876..7895)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8689..9277
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9178..9197
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9431..9448
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9688..9709
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9724..9729,9730..9747,9748..9754)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9762..9778
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9767..9789
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9786..9802
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9786..9802
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9852..9871
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagttct
     2221 tcactcaagt ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
     2281 tgatagagaa cgatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
     2341 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt
     2401 atgtcgagtt tatccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
     2461 atagagaacg tatgtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt
     2521 ttagtgaacc gtcagatcgc ctggagaaaa gcgctagcgc taccggtcgc caccatggtg
     2581 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     2641 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     2701 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     2761 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     2821 gacttcttca agtccgccat gcccgaaggc tacgtccagg cacgtagcag gccagggcct
     2881 ctctcagcca cctgagcaga aagctttcca agatagggca ggctgggtta ggccatctga
     2941 gtctgtctcg ttcattggga tccagacttg actgtcttgt taaaggctgt tgctgcccag
     3001 gtgtgcaggg agctgttggt ctctggcatt cagggtgggg gtggtataaa cccggggcag
     3061 cttgcatatg gcagggaaga gggatccgtg gaggaacagt gcagaaggct ttatgttcag
     3121 aatctctctt gcttttcttc tagactgagt tccttgagat tggtgaatgc tgtgtattat
     3181 tcatccctga taacctggtg tttggcccag ggccttgtcc agaggagtgt ttgataagtg
     3241 tttcaagtga attagcacca cgatgtcatc tcttttcagt ttacaaagga cggacacccc
     3301 ctgacccggt ctcagaaagc ctgaaagcag aattagtcat tagaagggtg gttggcttgg
     3361 tcggcataga ctttgagcag aaagaggttg aaaatgttga gcctgatttc tcttaggccc
     3421 ctctgcagtg tctgttgtgg aggccagata cgtaactgct tccgcttttt ttggtctcat
     3481 tcaaggtgag caaatcccct tcatgtttct caccagacaa tgcagctgat gaggttccag
     3541 ctttgcaaat gtagtcatcc atgaggactg tcttcctgag atttcatcag gctcgaggga
     3601 cttgcaaagg actttaggtc cattgtcctt ttattcttag atacctcttt cactgagacc
     3661 ttttccttac ctcacctctc taggtgacca agggtggccc cctgcccttc gcctgggaca
     3721 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     3781 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     3841 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     3901 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     3961 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     4021 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     4081 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     4141 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     4201 gccactccac cggcggcatg gacgagctgt acaaataagg tttagtgaac ggcgagatca
     4261 acttcagatc ttgataactc gagcctctcc ctcccccccc cctaacgtta ctggccgaag
     4321 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     4381 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     4441 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     4501 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     4561 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     4621 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     4681 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     4741 atctgatctg gggcctcggt acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     4801 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     4861 ccacaaccct ctagaatggt gagcaagggc gaggaggata acatggccat catcaaggag
     4921 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     4981 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgtgtggt
     5041 ccaaatggaa acctggtggg cagtggggct ctggggtgca aggtggaggc tagagaggaa
     5101 gagctgtgtt ttttttcctg acttacccag cagtggtctg tgagattgtc ttttctggtg
     5161 ggcgaacaaa aagggggtta ggaaaactca ggccaaaaaa gtgtaaggcg ttaattcccc
     5221 atttaattcc ttaaaatttc atgtaatacc aggtattgcc tgtaaaggaa agataaaggg
     5281 aaaaataagt aagaccttgt taaaatttta tttttctatt ttaaccttca cttatttcct
     5341 aattattaaa agaaatttat gcttattgtt aagaacaaaa aaatttcagt attacaatga
     5401 atttttaatt aaaagttttt ggcctgatga aatctcagga agacagtcct catggatgac
     5461 tacatttgca aagctggaac ctcatcagct gcattgtctg gtgagaaaca tgaaggggat
     5521 ttgctcacct tgaatgagac caaaaaaagc ggaagcagtt acgtatctgg cctccacaac
     5581 agacactgca gaggggccta agagaaatca ggctcaacat tttcaacctc tttctgctca
     5641 aagtctatgc cgaccaagcc aaccaccctt ctaatgacta attctgcttt caggctttct
     5701 gagaccgggt cagggggtgt ccttaaaggt tggaaaaaac ttttcctgtc atctttgcct
     5761 ccaaaatctg gctttctccc ttgggcaggg aaacctcccc aacatttctc tatcatccct
     5821 gagatgtggg gcctgcactc tgacttctgt ctgccttact ctttgtctta caggagcgca
     5881 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     5941 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     6001 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     6061 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     6121 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     6181 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     6241 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     6301 acaagtaagg atccaccgga tctagataac tgatcataat cagccatacc acatttgtag
     6361 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     6421 atgcaattgt tgttgttaac cgatagatcc taatcaacct ctggattaca aaatttgtga
     6481 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6541 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6601 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6661 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6721 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6781 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6841 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6901 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6961 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     7021 cctttgggcc gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta
     7081 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     7141 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     7201 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7261 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7321 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     7381 ttataacttg caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg
     7441 gctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     7501 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     7561 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7621 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7681 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7741 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7801 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7861 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     7921 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7981 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     8041 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     8101 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     8161 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     8221 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8281 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8341 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8401 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8461 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8521 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8581 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8641 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8701 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8761 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8821 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8881 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8941 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     9001 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     9061 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9121 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9181 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9241 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9301 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9361 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9421 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     9481 tctccccgcg cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt
     9541 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     9601 ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg
     9661 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     9721 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     9781 tcacacagga aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact
     9841 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
     9901 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
     9961 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
    10021 aagaaattgt atttgttaaa tatgtactac aaacttagta gt
//
