LOCUS       sequence-178055-       11178 bp    DNA     circular SYN 15-APR-2025
DEFINITION  expression pattern of AtFLS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11178)
  AUTHORS   Beck M, Wyrsch I, Strutt J, Wimalasekera R, Webb A, Boller T, 
            Robatzek S
  TITLE     Expression patterns of flagellin sensing 2 map to bacterial entry 
            sites in plant shoots and roots.
  JOURNAL   J Exp Bot. 2014 Dec;65(22):6487-98. doi: 10.1093/jxb/eru366. Epub 
            2014 Sep 9.
  PUBMED    25205577
REFERENCE   2  (bases 1 to 11178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/jxb/eru366"; journalName: "J Exp Bot"; date: "2014-12"; 
            volume: "65"; issue: "22"; pages: "6487-98"
COMMENT     Sequence Label: AtFLS2pro::GUS
FEATURES             Location/Qualifiers
     source          1..11178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1026..2837
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     primer_bind     complement(2847..2864)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2847..2863)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2856..2878)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3066..3090
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4391..5020
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5449..6522
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6588..6782
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7018..7040)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7126..7266
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7181..7200
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7281..7298)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7452..8040)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7532..7551)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8127..8921)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9346..9370
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9448..9622
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9679..10476)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9790..9809)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10400..10419
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(10545..10890)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10582..10602)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    11081..11102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11117..11122,11123..11140,11141..11147)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11155..11171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(11160..11178,1..4)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 caggaaacag ctatgacatg attacgaatt cgaagttgtg aattgtgatc ataggagtaa
       61 tatttctatc tgaacaaaaa ttgaattttg ttttgctttt tgttgaaaga taaataaaat
      121 gtttggcaga ttgtttgtac agaacacaac ttgttgataa aacaaattcc atcaagacag
      181 ctaatgagct tttgatagtc agagctttga agacgtgtta atgaattaaa ctgattagac
      241 ctaaaaccaa atatttagaa agaaaaaaat atgataattg ttatgttatt taatcaatag
      301 tcaaatacta tgttgagtac caatcatttc agtttcaacg attgctactc ttttgaccaa
      361 taacaaaaag gtttttattt ttcatttctc aaactccatt tttgtttgct tttagcataa
      421 aaacagctag aatgtgagtt tcatatgtat tatatactat aagaaatcat ataaattaac
      481 gactagaaca tattgttttc attctagtgg tatatctgag tttgactaga acgtttggag
      541 gcagattaaa gtgatttcgc cttgtcaaaa ctactttgta tatgttatca atcaatcgct
      601 caaaactaaa tcggaaaaaa actacataaa taagatatgg aaaatgatac taacacaaac
      661 tagtaagtaa gtagtcacta catattctgg tcccatgaat atggcttaca tagtacatac
      721 attgtgagat taaatgaata tgatatataa tacaagcata taagcatgtg catgactatg
      781 aagtgggaat acatttagtg cctttatatg caacttgaca gaattaccaa ttatgtgaat
      841 agtgaaactt atattgcgtc ttggcctttt cacatccgtg acatgatccc tcaaatctat
      901 atcttcttgc ggcatatgct tatatataaa catatgactt cgtctataaa tttatatcca
      961 gataattaat tctctcataa tatcttctcc acaatttcaa ctcttctaaa gtctaaaccg
     1021 gatccatgtt acgtcctgta gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt
     1081 gggcattcag tctggatcgc gaaaactgtg gaattgatca gcgttggtgg gaaagcgcgt
     1141 tacaagaaag ccgggcaatt gctgtgccag gcagttttaa cgatcagttc gccgatgcag
     1201 atattcgtaa ttatgcgggc aacgtctggt atcagcgcga agtctttata ccgaaaggtt
     1261 gggcaggcca gcgtatcgtg ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg
     1321 tcaataatca ggaagtgatg gagcatcagg gcggctatac gccatttgaa gccgatgtca
     1381 cgccgtatgt tattgccggg aaaagtgtac gtatcaccgt ttgtgtgaac aacgaactga
     1441 actggcagac tatcccgccg ggaatggtga ttaccgacga aaacggcaag aaaaagcagt
     1501 cttacttcca tgatttcttt aactatgccg gaatccatcg cagcgtaatg ctctacacca
     1561 cgccgaacac ctgggtggac gatatcaccg tggtgacgca tgtcgcgcaa gactgtaacc
     1621 acgcgtctgt tgactggcag gtggtggcca atggtgatgt cagcgttgaa ctgcgtgatg
     1681 cggatcaaca ggtggttgca actggacaag gcactagcgg gactttgcaa gtggtgaatc
     1741 cgcacctctg gcaaccgggt gaaggttatc tctatgaact gtgcgtcaca gccaaaagcc
     1801 agacagagtg tgatatctac ccgcttcgcg tcggcatccg gtcagtggca gtgaagggcg
     1861 aacagttcct gattaaccac aaaccgttct actttactgg ctttggtcgt catgaagatg
     1921 cggacttgcg tggcaaagga ttcgataacg tgctgatggt gcacgaccac gcattaatgg
     1981 actggattgg ggccaactcc taccgtacct cgcattaccc ttacgctgaa gagatgctcg
     2041 actgggcaga tgaacatggc atcgtggtga ttgatgaaac tgctgctgtc ggctttaacc
     2101 tctctttagg cattggtttc gaagcgggca acaagccgaa agaactgtac agcgaagagg
     2161 cagtcaacgg ggaaactcag caagcgcact tacaggcgat taaagagctg atagcgcgtg
     2221 acaaaaacca cccaagcgtg gtgatgtgga gtattgccaa cgaaccggat acccgtccgc
     2281 aaggtgcacg ggaatatttc gcgccactgg cggaagcaac gcgtaaactc gacccgacgc
     2341 gtccgatcac ctgcgtcaat gtaatgttct gcgacgctca caccgatacc atcagcgatc
     2401 tctttgatgt gctgtgcctg aaccgttatt acggatggta tgtccaaagc ggcgatttgg
     2461 aaacggcaga gaaggtactg gaaaaagaac ttctggcctg gcaggagaaa ctgcatcagc
     2521 cgattatcat caccgaatac ggcgtggata cgttagccgg gctgcactca atgtacaccg
     2581 acatgtggag tgaagagtat cagtgtgcat ggctggatat gtatcaccgc gtctttgatc
     2641 gcgtcagcgc cgtcgtcggt gaacaggtat ggaatttcgc cgattttgcg acctcgcaag
     2701 gcatattgcg cgttggcggt aacaagaaag ggatcttcac tcgcgaccgc aaaccgaagt
     2761 cggcggcttt tctgctgcaa aaacgctgga ctggcatgaa cttcggtgaa aaaccgcagc
     2821 agggaggcaa acaatgaaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa
     2881 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     2941 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     3001 tgctagagca gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca
     3061 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat
     3121 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
     3181 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca
     3241 gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa
     3301 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt
     3361 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc
     3421 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa
     3481 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc
     3541 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg
     3601 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag
     3661 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc
     3721 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc
     3781 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt
     3841 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag
     3901 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac
     3961 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc
     4021 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag
     4081 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga
     4141 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg
     4201 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca
     4261 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa
     4321 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca
     4381 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac
     4441 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga
     4501 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac
     4561 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga
     4621 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc
     4681 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga
     4741 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt
     4801 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc
     4861 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac
     4921 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt
     4981 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa
     5041 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct
     5101 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg
     5161 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag
     5221 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag
     5281 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc
     5341 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgc
     5401 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc
     5461 aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg
     5521 aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg
     5581 tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg
     5641 ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc
     5701 tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg
     5761 aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag
     5821 gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg
     5881 gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc
     5941 cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga
     6001 aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg
     6061 cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg
     6121 attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag
     6181 ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt
     6241 caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc
     6301 cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc
     6361 agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac
     6421 ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc
     6481 taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta
     6541 gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg
     6601 tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag
     6661 ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat
     6721 ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca
     6781 taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg
     6841 cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg
     6901 gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac
     6961 cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     7021 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     7081 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     7141 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     7201 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     7261 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7321 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7381 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7441 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7501 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7561 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7621 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7681 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7741 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7801 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7861 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     7921 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7981 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8041 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8101 gggattttgg tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt
     8161 attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct
     8221 tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc
     8281 ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg
     8341 ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa
     8401 aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc
     8461 acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta
     8521 ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac
     8581 agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca
     8641 tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt
     8701 ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag
     8761 gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc
     8821 agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg
     8881 atcagttttt tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt
     8941 ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac
     9001 tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa
     9061 agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag
     9121 caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa
     9181 acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg
     9241 ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg
     9301 tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg
     9361 tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta
     9421 ctgaattaac gccgaattaa ttcgggggat ctggatttta gtactggatt ttggttttag
     9481 gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact aagggtttct
     9541 tatatgctca acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta
     9601 ctcacacatt attatggaga aactcgagct tgtcgatcga ctctagctag aggatcgatc
     9661 cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
     9721 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     9781 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     9841 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     9901 ggcatcgcca tgtgtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc
     9961 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
    10021 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
    10081 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
    10141 ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag
    10201 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
    10261 ggccagccac gatagccgcg ctgcctcgtc ctggagttca ttcagggcac cggacaggtc
    10321 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cgaaacacgg cggcatcaga
    10381 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
    10441 agaacctgcg tgcaatccat cttgttcaat ccccatggtc gatcgacaga tctgcgaaag
    10501 ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga
    10561 aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc
    10621 cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct
    10681 tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag
    10741 gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc
    10801 ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt
    10861 cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct
    10921 ttgatattct tggagtagac gagagtgtcg tgctccacca tgttggcaag ctgctctagc
    10981 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
    11041 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    11101 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    11161 gcggataaca atttcaca
//
