LOCUS       sequence-178173-       10103 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Tissue-specific FLP expression plasmid for C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10103)
  AUTHORS   Munoz-Jimenez C, Ayuso C, Dobrzynska A, Torres-Mendez A, Ruiz PC, 
            Askjaer P
  TITLE     An Efficient FLP-Based Toolkit for Spatiotemporal Control of Gene 
            Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2017 Aug;206(4):1763-1778. doi: 
            10.1534/genetics.117.201012. Epub 2017 Jun 23.
  PUBMED    28646043
REFERENCE   2  (bases 1 to 10103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.117.201012"; journalName: "Genetics"; date: 
            "2017-08"; volume: "206"; issue: "4"; pages: "1763-1778"
COMMENT     Sequence Label: pBN258
FEATURES             Location/Qualifiers
     source          1..10103
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      287..875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     776..795
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1002..1024
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1021..1037
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1021..1037
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1057..1077
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2538..2562
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2711..2722)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(8027..8051)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9098..9118)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(9126..9143)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9126..9142)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9135..9157)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9470..9492
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9530..9548)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9616..9720
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9939..9958)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
       61 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
      121 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
      181 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
      241 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
      301 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
      361 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
      421 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
      481 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
      541 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
      601 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
      661 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
      721 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
      781 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
      841 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
      901 ggttcctggt cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
      961 ctgtggataa ccgtattacc gcctttgagt gagctgatac cagcggataa caatttcaca
     1021 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttggag
     1081 ggacttccag ctgtctgcga gccagtccaa aagggagagc caggatccac cggagagcca
     1141 ggaccagtcg gaccaccagg accatccgga caaccaggaa acgacggaac cccaggtaat
     1201 catttcatag tttttcaatc catatctaat caccacatct ttcaggacaa cctggaccaa
     1261 agggaccacc aggaccagac ggcaagccag gagccgacgg aaacccagga cagccaggac
     1321 cagtcggacc accaggaacc ccaggagagc gtggaatctg tccaaagtac tgcgccatcg
     1381 atggaggaat cttcttcgag gacggaactc gccgctaagc ggatcgcctt taatttattc
     1441 tttgccttat gcgttgctca aaataaaaca ttatttcatc caaaataatt ttttgaggtt
     1501 ttagatgaat ttgatagttt agcttgtcac tttctcctgt gcgaagaaac ttgctaagca
     1561 ggtaagcagc atccttatca agtttcgacc ttatctctca gtagtcgcat acgtcgtatc
     1621 cctcatctgc tttgttttag ccacttcaag ggaattcaga tcactttttc tcatgtgttt
     1681 tcaaatttac ttttgtggag tgaccttaat tgaccctctc gtcataagtg gacggtgggt
     1741 agttacttaa caaatttcga gtacaggata gtgtcaatgg catgagacat gtagtagcca
     1801 agcaattatg gcgaataagt ttcaaaaacc aacattctgt caaatacaaa gacctgcaac
     1861 attagagaat tcattaaata catcttcgag gaaatcaaat tttcccgtag acgatcccgt
     1921 agacctactt gtacaatatg ttctagatat acactccgaa cttaaaatat ctcgaacatt
     1981 tgtgttgaat atttatttta attctaggtt tttcaaattc cgcacgattt ttgccatact
     2041 actgcccaat agtttacatt tattgggcct ggatctgtaa ggcacttttg ggagctccct
     2101 ttcttttcct tcaaattacc catacagatc tataaatcac aatttaatac aagattttat
     2161 tcaagttaaa aacataaaag gggctttgcg gtggtcccca tttcaccaga gaactataaa
     2221 aaatataaat ttaacatcaa atgttgcaat tgattccctc tcaaagctgc ttgaagaacc
     2281 ctgattctgt caagcctatg aagatttaaa aaaaattggg aagaccctta gttccaaaca
     2341 agtgtcgttg acccagtagg cgatattctg aaaagtcata aaatggggtt gctaaaaaat
     2401 tggtcgctaa cttacattta gctaggaatg ttaattggaa taactcataa tttacagtaa
     2461 aattatcaaa aaatagcaaa aatcaataga ggaccgctac acaggcatcc aacagtacga
     2521 gttctgcgtt tttgaattca agtttgtaca aaaaagcagg ctctttgagc caatttatcc
     2581 aagtccttgt aaaaagtatt cgaaaattgt taacggataa aatgtttatt ataatatcaa
     2641 aaacaatttg tcagttgacc actttttgat atatttgaca gaaaccggga tgaattggct
     2701 caaagttagg gcgcccttct attacagggt ttctgataac aaaccggtta ttaactccca
     2761 acaagggatg atttcaattc atcatgctca aattgaccca aattaagtta catgacaaat
     2821 tcatcgccct tttcaactct tcttggctca tcatctgtta ttctgttcct attctgtctg
     2881 caccctatac ccctttgcat actctctcgt cattcctttc ttggatagtg tcttcttctc
     2941 cccagctctg ctacttctat gacttgcgcc cgctgctttt ccgctcgttc ctctcctctc
     3001 tgacgtcttc ttctgcttct tttctccttc ctttttccct tctcaatctt cctctttttc
     3061 ccatttctgt caatcattcg aagaagaaga agaagaagac cctcattcat ttcatttttt
     3121 tttctgtcgg tgtgtgctgc tcggttaaga gtgagctctc tattccacgt cttcttcttt
     3181 ttcttcttcg attcgaatca atcactccac caaaacgcat tcgtttttgg gattcacccc
     3241 gcggttcgca ataggtttct ttttcaaatt attagcgtta taaatagaaa aatggtggag
     3301 tttcaataaa aatgataatt tacaaagtgt attttgatta catgtactca aaaaggttga
     3361 aatttttcat accagttttc cggaaatcca tctgatatca ttatcgtatt tttcttttta
     3421 aaaatgtttt caaaaaaaaa caaaatatag ctggttattt ggcaccctct aattaccatt
     3481 ttcctgtcac accacactct tttccttctt ccacttcttt ccgttttcag ccgcttccaa
     3541 ccaaaccgat atgaaagccg agcaacaacg atcgattcca cccggctcgg cgaccttccc
     3601 gtcgcaggtg agactcagaa aactagagaa accgctcaac taactcttga tatccgattt
     3661 cattcttttc tcttttcttt ttgttgaact tttccatatt tccagatgcc acggccacca
     3721 ccaagcaccg aacaaggaat cacaacggaa tcggagcttg cgaagaaagc tcaaatcact
     3781 ccgaacgacg ttttagcact tccgggaatc actcaaggta tcttgctccc tttggatttc
     3841 agtttaaaac ataaatttag gattcttatg ctccccatct gcgaacatct ataacatcga
     3901 gttcaccaag ttccaaatcc gtgatctgga cactgagcaa gtgcttttcg agatcgccaa
     3961 accggagaac gatcaggaga atgatgagtc gccacaggag tcggcaagat acgtgcgtta
     4021 tagatttgct ccaaactttt tgaaactcaa aacggtcgga gcaactgtgg aattcaaagt
     4081 aggagacatc ccaatccatc atttccgaat gatcgaacgt cacttcttca aagatcgcct
     4141 tctgaagtgt tttgactttg aattcggatt ctgtattccg aattcacgaa acaactgtga
     4201 acatatctat gagttccctc aactctctca acaactcagt gagtcattat tctaaaaagt
     4261 acaattcaaa agactaatct ctttttcagt ggacgacatg atcaacaatc caaacgagac
     4321 tcgttctgac agcttctatt tcgtcgataa caaactcgtc atgcacaaca aagccgacta
     4381 ctcatatgat gcataaatat ttaatacaaa aatgttctgg ataattattc tgtcgaatag
     4441 aaaaaaaact ccaaatgtga ttaaattcca ataattcctg tctagtttgt tccttcccct
     4501 tccccttctc atgttcaatg cattcctaag cttttcagtt ccccccttgt ttctatattt
     4561 tttcgctgtc ctgtcacact cgctaaaaac actaatcaca cggaaatctg ttttcaataa
     4621 aaactccaac tttaactcat tttcaatttc aactgaaaga ttttttcatt agagaatgtc
     4681 tagaactagg cccggggaaa gccgggttac cataagaaaa aaaattacat tttttctcga
     4741 atagtagaaa catattgcaa gactattttt cattatttca gaacacacac tggagagaga
     4801 ccgtatccat gtgctcaatg tgagaaggca ttcaccgaca gctcaactct tacaaaacat
     4861 ctgagaaccc atactggaca taaaccttat gtttgctcaa tttgtatgat gaaattcaca
     4921 cagagtggga acttgcatag gcacatgaaa actcataaat agtttttcct attatggaag
     4981 taatgtttct aaatgaatat tgccattttc tgcaaaatat tttacaatcc acaagtctcg
     5041 aacaaattta atcaatgtct tgttttgata caatttaaca aattcactgt ggagaggatt
     5101 tactataaaa acgttttaca ccagcaaaaa atcaatagaa catttacatg ataaggagaa
     5161 tatataaaaa gggggcatta taatttactg atacaatatg gacaacattt gaaggatatg
     5221 cagatacata cgcgttagat acgacggttg atgtaggagg agaggtagtc gaggacctct
     5281 tgggagatga ttccgttcca ggctgggtaa cggatggatc cctcggcgga tcccttgagt
     5341 tgctcgatgt gttgccactc ctcgattggg ttggtctcgt ccttgagggc gatcatctcc
     5401 ttggagattg ggtcgtaggc gtagtaacgg gagacgaggg cgaagtagtg gtctgggatg
     5461 gcggtgattt ggtgggtgta ggtggtacgg gcgacggcgg aggcacgctt gtcggaccag
     5521 tttccgacga cgttggtgag ctcggtgagt ccctgaaaat ttaaatcaga ttagttagtt
     5581 agtaaaatca tgtttaaact taccttcatg gagaggaagg aggtcatgag gtgacgtccg
     5641 atgtgggact ttggtccgtt cttgatggcg aagatggagt atggggcgtt cttcttgagg
     5701 gccttgttgt aggaacggac gaggttgtcc ttgaggagtt ggtactcttg cttgttggag
     5761 gaggagtttc cggtacggtt gacacgcttg aggactggct cggagttacg gaggaactcg
     5821 tcgaggtaga cgagtgggtc gatacgtcca cgggcggaga agaagtagat gtgacgggag
     5881 acggaggtct tggtctcggt gacgaggcat tggatgatga ctccgaggta cttgttttgg
     5941 acgagcttga aggactttgg gtcgacgttc ttgatgtcgg agaaacgtcc gcagttgatg
     6001 aaggtggcga ggaagaggaa ttggtagagg gtcttggtct tggtgaaacg ggaggtgtac
     6061 tcgaaggagt tgaggatctt ctcggtgatc tcccagatgg actctccctc ggagaggagg
     6121 gccttgagca tcttcttgga gtgggagttt cccttgtcgg cctcctcgga ggactcgaat
     6181 tggagttgga gggaggagac gatgtcggtg atgtcggatt ggtgcttttg tccgtagtat
     6241 gggatgatgg tgaactccca ggctgggatg agcttctgaa aatttaaata atcagggtta
     6301 gttagtatat atatgtttaa acttaccttg agggaggcct cgaggatggt ggccttttgg
     6361 gtcttgtact tgaattggag ggacttgttg acgatgtcga aggagaggga gttggagatg
     6421 atggtgttgt aggacatgaa ggtggcacgc ttgatggcgg ttccgttgtg ggtgatcatc
     6481 cagcagaggt aggtgagctc ggcggcgcag agggcgatct tctctccgga tggacgctcg
     6541 aaacgctcga cgaattgacg gacgaggact tttggtggtg tcttacagag aatgtcgaat
     6601 tgtggcattt ttggtaccat atgctgttgt agcttttaac taactaaaat gaaaaagaaa
     6661 ttccaacaaa aaataaatga gttccgtgtg ctcttgctcg cagagcacat ccaaaaatta
     6721 atgggggtcg tccgtaatga ttgacagaga tggaaaatgg cgaaaaaggg ctgaagaaga
     6781 tgaaaaaaga agacgtctag gaaaagagaa gcgagagagt gtggggaacg ctgagggcgc
     6841 agagagagga acggacaaaa tgacagatga tgagcaaaga gaaagagaga gagggagaga
     6901 gaaagagagg gagagaggga tgaatttgtg atgaaataga cagtgagaga aaatgagagg
     6961 aaaagatggt aattcaatag aatcttagag caatttgaag tttgagtcat tacttttaaa
     7021 gtgggcatct ttaaaggtag aatcatttag gattttatct aaataaatct ataataaacc
     7081 aatatgactg aatgtcattt tggtacactg ccaaaattca aaaaaaaatt ggagaatttg
     7141 agaaattttg agctttgcta ccagtttgaa cctctggaac aggggtgggc ggcaattgcc
     7201 ttccggcaaa tttcgccaaa ttgccggttt gccgatttgt cggaaatttt aattttcggc
     7261 aaaaatgcgg atttgctctt ttacggatat caatttgccg gaagttttta gagggattta
     7321 gtataattcg gaaacacttg aaactgtgcc tttttgaaat tgtttcccca ttttctttag
     7381 acattttcat agaattcgct tacttttcaa aatagatgta ggaacattct taggatgcct
     7441 acagttttgc cgattaaaat tgaaattctg aaatttccaa aaaaaaaaat tttaaaacca
     7501 caatttgcca ttttcgtgtc aaaaactttc ggcaattacc gtttttccgg caaattcagc
     7561 aaattggcaa tttgccggtt ccccaatttg ccggaaattt tcagttctgg caatttgcca
     7621 atggtttttg aaacatttta cagtaatata taaggatttc atgggtaact ataggactat
     7681 aggactatag gagtggttta aaaaactgaa ttttgagaga cttgacacat tttccaaaac
     7741 atttttttta atgttacgtt tgacttaaag caatgataag atatgttaag attttgaaga
     7801 atttagatat gaggttatag aatactttgc attagtgatg tatttatttg aaagaacatt
     7861 gatggggaga atttttcaaa cagaaaaaaa actataaaat tctacttctt ttactgaagc
     7921 ttatcgatac cgtcctgcag gtagcggccg catgctacag ccggctcctc gagacgtacg
     7981 gtgcgcgcga tgcattcgaa gatctgccca ctagtgagtc gtattaaccc agctttcttg
     8041 tacaaagtgg atagaatcaa gcatgctccg cgtttgctgt actatttctc gcagagcagt
     8101 tttttttctc acactttatt attctgtact gcccctccac gctataattt tttgcataga
     8161 gggccataaa ggaaaacggt acggtaaaaa taatgggtcc tttttaccgt tgtgtagatt
     8221 gtttctaacg ggatgcaaat tttcactaaa actctggaaa actgatggaa ttctacgggc
     8281 agccggcaat tttagatatt tgaagaatcc ttcttgatca gtgccaagct ttcccatgga
     8341 ccaaagatgt ggatccaaga tcaaatgcac aggatctgaa ctcctttgtt ccaaaaacaa
     8401 ggatttcttt tctatagttt gacctaaata ttatagtaga acatatacat ttgatgattt
     8461 catgaataat ttattgttaa aaaataatac atgtgttaaa aaataattct aaaaaattaa
     8521 ttggagcgtc cttggcggat atggcggatt ggaaggtcat aagtaactcg aactaacctg
     8581 taaatataat taatttagaa gtaatcattt tgaaatgttt ctaaattttt gtaaacgtaa
     8641 tcaatgattt acttacaaaa tttttcgaaa tattagtaga aatataaaaa accagtaaaa
     8701 ttcaaaagag gccagccaca catgcatctt acaatacgaa tgatgcgttt tttgtcgcct
     8761 cggagaagga atcaagcagg ctccgcgttt tctgtactat ttctcccaga gccgtttatt
     8821 ttgcataggg ggtgggtcat aaacggagac gttttggtat aaaatttgcc ccttcttcca
     8881 ccgttgaaaa aattggaaaa tgaaccggga caggaaaggg gatttcagga taatggttca
     8941 attatcgatg ctactgtcat atctgagtag tggatttgag caaggtttcc aaattttgag
     9001 tacaagcgtc tagtgcatgt gtcaaaaaag tcgggcaaaa attagtttta atcaataagg
     9061 tttaaagatc agaaattttt gtctgaccta atgggcccaa ctttattata catagttgat
     9121 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     9181 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     9241 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
     9301 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tgcaatctgc
     9361 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     9421 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     9481 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     9541 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     9601 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     9661 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     9721 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     9781 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     9841 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     9901 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     9961 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
    10021 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
    10081 tgcagtgctg ccataaccat gag
//
