LOCUS       sequence-178403-        9512 bp    DNA     circular SYN 14-APR-2025
DEFINITION  To convert human skin fibroblasts into induced cholinergic neurons 
            (hiCN) in combination with FGF2 and two small molecules, forskolin 
            and dorsomorphin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Liu ML, Zang T, Zou Y, Chang JC, Gibson JR, Huber KM, Zhang CL
  TITLE     Small molecules enable neurogenin 2 to efficiently convert human 
            fibroblasts into cholinergic neurons.
  JOURNAL   Nat Commun. 2013;4:2183. doi: 10.1038/ncomms3183.
  PUBMED    23873306
REFERENCE   2  (bases 1 to 9512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2183. doi"; pages: " 
            10.1038/ncomms3183"
COMMENT     Sequence Label: pLNCX2-NGN2-IRES-GFP-T2A-Sox11
FEATURES             Location/Qualifiers
     source          1..9512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        28..331
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        332..535
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     486..506
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    1419..1985
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1436..1987
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1580..1597)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1807..1826
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(1990..1992,1993..1995,1996..2706)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1990..1992,1993..1995,1996..2706)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2035..2056)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2296..2315)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2643..2664
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2725..2778
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2803..2812
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             4065..4658
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4761..4783)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4869..5009
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4924..4943
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5024..5041)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5195..5783)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5275..5294)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5954..6745,6746..6814))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6577..6596
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6815..6919)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6987..7005
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             7577..7752
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    7815..8014
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             8215..8631
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     8561..8583
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     8599..8615
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             8675..9469
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8729..8748)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9339..9358
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
       61 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      121 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      181 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      241 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      301 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      361 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      421 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      481 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
      541 agtgaaccgt cagatccgct agcgctaccg gactcagatc caccggtgcc accatgttcg
      601 tcaaatccga gaccttggag ttgaaggagg aagaggacgt gttagtgctg ctcggatcgg
      661 cctcccccgc cttggcggcc ctgaccccgc tgtcatccag cgccgacgaa gaagaggagg
      721 aggagccggg cgcgtcaggc ggggcgcgtc ggcagcgcgg ggctgaggcc gggcaggggg
      781 cgcggggcgg cgtggctgcg ggtgcggagg gctgccggcc cgcacggctg ctgggtctgg
      841 tacacgattg caaacggcgc ccttcccggg cgcgggccgt ctcccgaggc gccaagacgg
      901 ccgagacggt gcagcgcatc aagaagaccc gtagactgaa ggccaacaac cgcgagcgaa
      961 accgcatgca caacctcaac gcggcactgg acgcgctgcg cgaggtgctc cccacgttcc
     1021 ccgaggacgc caagctcacc aagatcgaga ccctgcgctt cgcccacaac tacatctggg
     1081 cactcaccga gaccctgcgc ctggcggatc actgcggggg cggcggcggg ggcctgccgg
     1141 gggcgctctt ctccgaggca gtgttgctga gcccgggagg cgccagcgcc gccctgagca
     1201 gcagcggaga cagcccctcg cccgcctcca cgtggagttg caccaacagc cccgcgccgt
     1261 cctcctccgt gtcctccaat tccacctccc cctacagctg cactttatcg cccgccagcc
     1321 cggccgggtc agacatggac tattggcagc ccccacctcc cgacaagcac cgctatgcac
     1381 ctcacctccc catagccagg gattgtatct agatgaattc cgcccccccc ccctaacgtt
     1441 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     1501 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     1561 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     1621 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     1681 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     1741 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     1801 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     1861 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     1921 ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     1981 gataatacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2041 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2101 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2161 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     2221 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2281 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2341 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2401 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     2461 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     2521 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2581 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     2641 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     2701 tacaagtccg gactcagatc ctacgagggg agaggaagtc ttctgacctg cggagacgtc
     2761 gaagagaatc ctggacccgc tagcgctacc ggactcagat ccgccaccat ggtgcagcag
     2821 gccgagagct cggaagccga gagcaacctg ccccgggacg cgctggacac cgaggagggc
     2881 gagttcatgg cgtgcagccc ggtggccctg gacgagagcg acccggactg gtgcaagacg
     2941 gcgtcgggcc acatcaaacg gcccatgaac gccttcatgg tgtggtccaa gatcgagcgc
     3001 aggaagatca tggagcagtc gcccgacatg cacaacgccg agatctccaa gaggctgggc
     3061 aagcgctgga agatgctgaa ggacagcgag aagatcccgt tcatcaggga ggcggagcgc
     3121 ctgcgcctca agcacatggc tgattatccc gactacaagt accggccgcg caaaaagccc
     3181 aagacggacc cagcggccaa gcccagcgcg ggccagagcc ccgacaagag cgcggcgggc
     3241 gccaaggcag ccaagggccc cggcaagaag tgcgccaagc tcaaggcgcc tgcgggcaag
     3301 gcgggcgcgg gcaaggcggc gcagccgggg gactgcgccg cgggcaaggc agccaagtgc
     3361 gtcttcctgg acgacgacga tgaagacgac gacgaagatg acgagctgca gctacggccc
     3421 aagccggacg ctgacgacga cgacgacgag cccgcgcact cgcacctgct gccgccgccg
     3481 acgcagcagc aaccccctca gctgctgagg cgctacagcg tggccaaggt ccccgccagc
     3541 cccacgctca gcagtgccgc cgagtccccc gagggcgcga gcctgtacga cgaagtgcgc
     3601 gcgggcggcc ggctctacta cagcttcaag aacatcacca agcagcagcc tccgcccgcg
     3661 cctcccgcgc tgtcgcccgc gtcctcccgc tgcgtgtcca cctcctcatc cagcggcagc
     3721 agcagcggca gcggcgccga ggatgcagac gacctcatgt tcgacctgag cttgaatttc
     3781 tcccagggcg cgcacagcgc ctgcgagcag ccactgggcg cgggagcggc ggggaacctg
     3841 tccctgtcgc tggtggataa ggacctggat tccttcagcg agggcagcct gggttcccac
     3901 ttcgagttcc ccgactactg cacgccggag ctgagcgaga tgatcgcggg ggactggctg
     3961 gaggcgaact tctccgacct ggtgttcacg tattgaagta cgctcgaggg atcaagctta
     4021 tcgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
     4081 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa
     4141 ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca
     4201 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca
     4261 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     4321 agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg
     4381 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     4441 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     4501 cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca
     4561 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct
     4621 ctgagtgatt gactacccgt cagcgggggt ctttcatttg ggggctcgtc cgggatcggg
     4681 agacccctgc ccagggacca ccgacccacc accgggaggt aagctggctg cctcgcgcgt
     4741 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     4801 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     4861 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     4921 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     4981 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     5041 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     5101 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5161 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5221 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5281 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5341 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     5401 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5461 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5521 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     5581 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     5641 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     5701 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     5761 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     5821 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     5881 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     5941 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     6001 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     6061 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     6121 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     6181 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     6241 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     6301 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     6361 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     6421 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     6481 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     6541 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     6601 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     6661 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     6721 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     6781 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     6841 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     6901 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     6961 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag
     7021 aattcatacc agatcaccga aaactgtcct ccaaatgtgt ccccctcaca ctcccaaatt
     7081 cgcgggcttc tgcctcttag accactctac cctattcccc acactcaccg gagccaaagc
     7141 cgcggccctt ccgtttcttt gcttttgaaa gaccccaccc gtaggtggca agctagctta
     7201 agtaacgcca ctttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
     7261 aaggtcagga acaaagaaac agctgaatac caaacaggat atctgtggta agcggttcct
     7321 gccccggctc agggccaaga acagatgaga cagctgagtg atgggccaaa caggatatct
     7381 gtggtaagca gttcctgccc cggctcgggg ccaagaacag atggtcccca gatgcggtcc
     7441 agccctcagc agtttctagt gaatcatcag atgtttccag ggtgccccaa ggacctgaaa
     7501 atgaccctgt accttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     7561 ttccgctctc cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcttcc
     7621 gatagactgc gtcgcccggg tacccgtatt cccaataaag cctcttgctg tttgcatccg
     7681 aatcgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccacgacgg
     7741 gggtctttca tttgggggct cgtccgggat ttggagaccc ctgcccaggg accaccgacc
     7801 caccaccggg aggtaagctg gccagcaact tatctgtgtc tgtccgattg tctagtgtct
     7861 atgtttgatg ttatgcgcct gcgtctgtac tagttagcta actagctctg tatctggcgg
     7921 acccgtggtg gaactgacga gttctgaaca cccggccgca accctgggag acgtcccagg
     7981 gactttgggg gccgtttttg tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc
     8041 ccgtcaggat atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct
     8101 gaatttttgc tttcggtttg gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc
     8161 atcgttctgt gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa ttagggccag
     8221 actgttacca ctcccttaag tttgacctta ggtcactgga aagatgtcga gcggatcgct
     8281 cacaaccagt cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg
     8341 ccaaccttta acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag
     8401 gttaagatca aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc
     8461 gtgacctggg aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct
     8521 aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg
     8581 accccgcctc gatcctccct ttatccagcc ctcactcctt ctctaggcgc cggaattccg
     8641 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     8701 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     8761 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     8821 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     8881 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     8941 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     9001 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     9061 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     9121 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     9181 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     9241 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     9301 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     9361 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     9421 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     9481 gggttcgaat agtaatcaat tacggggtca tt
//
