LOCUS       sequence-178443-        7812 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Focal Adhesion Kinase, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7812)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 7812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-PTK2-N-10
FEATURES             Location/Qualifiers
     source          1..7812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    182..303
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(219..238)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     273..292
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(310..765)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(447..468)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     659..678
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        792..896
                     /gene="bla"
                     /label=AmpR promoter
     promoter        898..1255
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        898..1089
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(921..941)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1106..1241
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1290..2084
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1290..2084
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1344..1363)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1954..1973
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(2272..2291)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2316..2363
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      2692..3280
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3181..3200
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        3455..3758
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3759..3962
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3913..3933
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3991..4000
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gttaagctgt atgagcatgc tgttgctcat tctggattgc ctgacaatgc cagacgataa
       61 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt
      121 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
      181 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
      241 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
      301 ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
      361 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
      421 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
      481 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
      541 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
      601 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
      661 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
      721 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
      781 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      841 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg
      901 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
      961 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     1021 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     1081 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     1141 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     1201 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg
     1261 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     1321 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     1381 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     1441 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     1501 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     1561 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     1621 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     1681 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     1741 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     1801 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     1861 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     1921 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     1981 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     2041 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     2101 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     2161 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     2221 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg
     2281 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     2341 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     2401 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
     2461 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
     2521 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt
     2581 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     2641 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2701 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2761 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2821 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2881 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2941 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3001 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3061 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3121 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3181 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3241 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3301 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3361 tgattctgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg
     3421 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     3481 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     3541 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     3601 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     3661 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     3721 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     3781 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     3841 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     3901 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     3961 ctggtttagt gaaccgtcag atccgctagc gccaccatgg cagctgctta ccttgacccc
     4021 cacttgaatc acacaccaaa ttcgagtact aagactcacc tgggtactgg tatggaacgt
     4081 tctcctggtg caatggagcg agtattaaag gtctttcatt attttgaaag caatagtgag
     4141 ccaaccacct gggccagtat tatcaggcat ggagatgcta ctgatgtcag gggcatcatt
     4201 cagaagatag tggacagtca caaagtaaag catgtggcct gctatggatt ccgcctcagt
     4261 cacctgcggt cagaggaggt tcactggctt cacgtggata tgggcgtctc cagtgtgagg
     4321 gagaagtatg agcttgctca cccaccagag gagtggaaat atgaattgag aattcgttat
     4381 ttgccaaaag gatttctaaa ccagtttact gaagataagc caactttgaa tttcttctat
     4441 caacaggtga agagcgatta tatgttagag atagctgatc aagtggacca ggaaattgct
     4501 ttgaagttgg gttgtctaga aatacggcga tcatactggg agatgcgggg caatgcacta
     4561 gaaaagaagt ctaactatga agtattagaa aaagatgttg gtttaaagcg attttttcct
     4621 aagagtttac tggattctgt caaggccaaa acactaagaa aactgatcca acaaacattt
     4681 agacaatttg ccaaccttaa tagagaagaa agtattctga aattctttga gatcctgtct
     4741 ccagtctaca gatttgataa ggaatgcttc aagtgtgctc ttggttcaag ctggattatt
     4801 tcagtggaac tggcaatcgg cccagaagaa ggaatcagtt acctaacgga caagggctgc
     4861 aatcccacac atcttgctga cttcactcaa gtgcaaacca ttcagtattc aaacagtgaa
     4921 gacaaggaca gaaaaggaat gctacaacta aaaatagcag gtgcacccga gcctctgaca
     4981 gtgacggcac catccctaac cattgcggag aatatggctg acctaataga tgggtactgc
     5041 cggctggtga atggaacctc gcagtcattt atcatcagac ctcagaaaga aggtgaacgg
     5101 gctttgccat caataccaaa gttggccaac agcgaaaagc aaggcatgcg gacacacgcc
     5161 gtctctgtgt cagaaacaga tgattatgct gagattatag atgaagaaga tacttacacc
     5221 atgccctcaa ccagggatta tgagattcaa agagaaagaa tagaacttgg acgatgtatt
     5281 ggagaaggcc aatttggaga tgtacatcaa ggcatttata tgagtccaga gaatccagct
     5341 ttggcggttg caattaaaac atgtaaaaac tgtacttcgg acagcgtgag agagaaattt
     5401 cttcaagaag ccttaacaat gcgtcagttt gaccatcctc atattgtgaa gctgattgga
     5461 gtcatcacag agaatcctgt ctggataatc atggagctgt gcacacttgg agagctgagg
     5521 tcatttttgc aagtaaggaa atacagtttg gatctagcat ctttgatcct gtatgcctat
     5581 cagcttagta cagctcttgc atatctagag agcaaaagat ttgtacacag ggacattgct
     5641 gctcggaatg ttctggtgtc ctcaaatgat tgtgtaaaat taggagactt tggattatcc
     5701 cgatatatgg aagatagtac ttactacaaa gcttccaaag gaaaattgcc tattaaatgg
     5761 atggctccag agtcaatcaa ttttcgacgt tttacctcag ctagtgacgt atggatgttt
     5821 ggtgtgtgta tgtgggagat actgatgcat ggtgtgaagc cttttcaagg agtgaagaac
     5881 aatgatgtaa tcggtcgaat tgaaaatggg gaaagattac caatgcctcc aaattgtcct
     5941 cctaccctct acagccttat gacgaaatgc tgggcctatg accccagcag gcggcccagg
     6001 tttactgaac ttaaagctca gctcagcaca atcctggagg aagagaaggc tcagcaagaa
     6061 gagcgcatga ggatggagtc cagaagacag gccacagtgt cctgggactc cggagggtct
     6121 gatgaagcac cgcccaagcc cagcagaccg ggttatccca gtccgaggtc cagcgaagga
     6181 ttttatccca gcccacagca catggtacaa accaatcatt accaggtttc tggctaccct
     6241 ggttcacatg gaatcacagc catggctggc agcatctatc caggtcaggc atctcttttg
     6301 gaccaaacag attcatggaa tcatagacct caggagatag caatgtggca gcccaatgtg
     6361 gaggactcta cagtattgga cctgcgaggg attgggcaag tgttgccaac ccatctgatg
     6421 gaagagcgtc taatccgaca gcaacaggaa atggaagaag atcagcgctg gctggaaaaa
     6481 gaggaaagat ttctgaaacc tgatgtgaga ctctctcgag gcagtattga cagggaggat
     6541 ggaagtcttc agggtccgat tggaaaccaa catatatatc agcctgtggg taaaccagat
     6601 cctgcagctc caccaaagaa accgcctcgc cctggagctc ccggtcatct gggaagcctt
     6661 gccagcctca gcagccctgc tgacagctac aacgagggtg tcaagccatg gaggcttcag
     6721 ccccaggaaa tcagcccccc tcctactgcc aacctggacc ggtcgaatga taaggtgtac
     6781 gagaatgtga cgggcctggt gaaagctgtc atcgagatgt ccagtaaaat ccagccagcc
     6841 ccaccagagg agtatgtccc tatggtgaag gaagtcggct tggccctgag gacattattg
     6901 gccactgtgg atgagaccat tcccctccta ccagccagca cccaccgaga gattgagatg
     6961 gcacagaagc tattgaactc tgacctgggt gagctcatca acaagatgaa actggcccag
     7021 cagtatgtca tgaccagcct ccagcaagag tacaaaaagc aaatgctgac tgctgctcac
     7081 gccctggctg tggatgccaa aaacttactc gatgtcattg accaagcaag actgaaaatg
     7141 cttgggcaga cgagaccaca cagctccgga ggggatccac cggtcgccac catgagtgcg
     7201 attaagccag acatgaagat caacctccgt atggaaggca acgtaaacgg gcaccacttt
     7261 gtgatcgacg gagatggtac aggcaagcct tttgagggaa aacagagtat ggatcttgaa
     7321 gtcaaagagg gcggacctct gccttttgcc tttgatatcc tgaccactgc attccattac
     7381 ggcaacaggg tattcgccga atatccagac cacatacaag actattttaa gcagtcgttt
     7441 cctaaggggt attcgtggga acgaagcttg actttcgaag acgggggcat ttgcattgcc
     7501 agaaacgaca taacaatgga aggggacact ttctataata aagttcgatt tcacggtgta
     7561 aactttcccg ccaatggtcc agttatgcag aagaagacgc tgaaatggga gccctccact
     7621 gagaaaatgt atgtgcgtga tggagtgctg actggtgata ttcgcatggc tttgttgctt
     7681 gaaggaaatg cccattaccg atgtgacttc agaactactt acaaagctaa ggagaagggt
     7741 gtcaagttac caggctacca ctttgtggac cactgcattg agattttaag ccatgacaaa
     7801 gattacaaca ag
//
