LOCUS       sequence-178617-        4458 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Cre in Stagia3 backbone; vector-Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Wang S, Sengel C, Emerson MM, Cepko CL
  TITLE     A gene regulatory network controls the binary fate decision of rod 
            and bipolar cells in the vertebrate retina.
  JOURNAL   Dev Cell. 2014 Sep 8;30(5):513-27. doi: 
            10.1016/j.devcel.2014.07.018. Epub 2014 Aug 21.
  PUBMED    25155555
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.07.018"; journalName: "Dev Cell"; date: 
            "2014-09-8- 8"; volume: "30"; issue: "5"; pages: "513-27"
COMMENT     Sequence Label: Stagia3-Cre
FEATURES             Location/Qualifiers
     source          1..4458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(49..637)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(129..148)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(808..1599,1600..1668))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1431..1450
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1669..1773)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    1846..1894
                     /note="synthetic polyadenylation signal"
     misc_feature    1908..1999
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     1948..1967
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      2108..2117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2114..3142
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MANLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             3152..3181
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3279..3298)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3344..3399
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3398..3417)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3760..3776)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3760..3776)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3773..3795)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3784..3800
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3808..3814,3815..3832,3833..3838))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3853..3874
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3933..4128
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3979..4114
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4134..4268
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4171..4190)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4225..4244
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4336..4353)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
       61 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      121 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      181 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      241 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      301 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      361 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      421 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      481 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
      541 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
      601 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
      661 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
      721 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
      781 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
      841 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
      901 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
      961 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     1021 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     1081 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     1141 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     1201 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     1261 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     1321 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     1381 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     1441 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     1501 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     1561 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     1621 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     1681 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     1741 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg
     1801 gtcgagaagt aatattaagg tacgggaggt acttggagcg gccgcaataa aatatcttta
     1861 ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc
     1921 catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca
     1981 ggtgccagaa catttctcta tcgatagtcg accaattgct cgaggaattc tatataatgg
     2041 aagcttgacc ggagccgtta gttgcccgtc ggacgcgatt tgcgaggcgc tttatgcgcg
     2101 caccggagcc accatggcca atttactgac cgtacaccaa aatttgcctg cattaccggt
     2161 cgatgcaacg agtgatgagg ttcgcaagaa cctgatggac atgttcaggg atcgccaggc
     2221 gttttctgag catacctgga aaatgcttct gtccgtttgc cggtcgtggg cggcatggtg
     2281 caagttgaat aaccggaaat ggtttcccgc agaacctgaa gatgttcgcg attatcttct
     2341 atatcttcag gcgcgcggtc tggcagtaaa aactatccag caacatttgg gccagctaaa
     2401 catgcttcat cgtcggtccg ggctgccacg accaagtgac agcaatgctg tttcactggt
     2461 tatgcggcgg atccgaaaag aaaacgttga tgccggtgaa cgtgcaaaac aggctctagc
     2521 gttcgaacgc actgatttcg accaggttcg ttcactcatg gaaaatagcg atcgctgcca
     2581 ggatatacgt aatctggcat ttctggggat tgcttataac accctgttac gtatagccga
     2641 aattgccagg atcagggtta aagatatctc acgtactgac ggtgggagaa tgttaatcca
     2701 tattggcaga acgaaaacgc tggttagcac cgcaggtgta gagaaggcac ttagcctggg
     2761 ggtaactaaa ctggtcgagc gatggatttc cgtctctggt gtagctgatg atccgaataa
     2821 ctacctgttt tgccgggtca gaaaaaatgg tgttgccgcg ccatctgcca ccagccagct
     2881 atcaactcgc gccctggaag ggatttttga agcaactcat cgattgattt acggcgctaa
     2941 ggatgactct ggtcagagat acctggcctg gtctggacac agtgcccgtg tcggagccgc
     3001 gcgagatatg gcccgcgctg gagtttcaat accggagatc atgcaagctg gtggctggac
     3061 caatgtaaat attgtcatga actatatccg taacctggat agtgaaacag gggcaatggt
     3121 gcgcctgctg gaagatggcg atggaccggt ggaacaaaaa cttatttctg aagaagatct
     3181 gtgagcatcg cggccggccg cactcctcag gtgcaggctg cctatcagaa ggtggtggct
     3241 ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat
     3301 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
     3361 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     3421 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
     3481 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
     3541 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
     3601 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta
     3661 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg
     3721 agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     3781 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     3841 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     3901 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc
     3961 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4021 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     4081 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt
     4141 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     4201 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     4261 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     4321 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     4381 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     4441 aaagaacatg tgagcaaa
//
