LOCUS       sequence-179037-        8863 bp    DNA     circular SYN 15-APR-2025
DEFINITION  pAC154-dual-dCas9VP160-sgExpression with MYRF sg1 cloned into BbsI 
            site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Pouladi M
  TITLE     Pouladi lab MYRF plasmids 2017
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 8863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pYTP MYRF sg1
FEATURES             Location/Qualifiers
     source          1..8863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      20..608
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     509..528
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        670..918
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     670..690
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     841..860
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        939..1014
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        1111..1396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        1398..1678
                     /label=chicken beta-actin promoter
     intron          1679..1906
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             1927..1953
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1957..1977
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1987..2010,2011..2013,2014..4569,4570..4572,
                     4573..6087)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1987..2010,2011..2013,2014..4500,4501..4503,
                     4504..6087)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6091..6111
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6145..6528
                     /codon_start=1
                     /product="10 tandem repeats of the minimal activation 
                     domain of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP160
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGS
                     DALDDFDLDMLGSDALDDFDLDML"
     primer_bind     complement(6573..6590)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6579..6786
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6795..6935
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6795..6924
                     /label=AAV2 ITR
     rep_origin      7010..7465
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7097..7116)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7307..7328
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7482..7501)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7601..7623
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7661..7679)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7747..7851
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7852..7920,7921..8712)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8070..8089)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
       61 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
      121 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      181 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      241 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      301 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      361 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      421 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      481 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      541 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      601 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      661 ctcacatgtg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
      721 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
      781 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
      841 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
      901 tggaaaggac gaaacaccgg caagcgcggc ggcggaccgt tttagagcta gaaatagcaa
      961 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
     1021 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgtttttagc gcgtgcgcca
     1081 attctgcaga caaatggctc tagaggtacc cgttacataa cttacggtaa atggcccgcc
     1141 tggctgaccg cccaacgacc cccgcccatt gacgtcaata gtaacgccaa tagggacttt
     1201 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     1261 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     1321 ttgtgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     1381 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
     1441 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
     1501 gggggggggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
     1561 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
     1621 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
     1681 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
     1741 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
     1801 gggctgtaat tagctgagca agaggtaagg gtttaaggga tggttggttg gtggggtatt
     1861 aatgtttaat tacctggagc acctgcctga aatcactttt tttcaggttg gaccggtgcc
     1921 accatgtacc catacgatgt tccagattac gcttcgccga agaaaaagcg caaggtcgaa
     1981 gcgtccgaca agaagtacag catcggcctg gccatcggca ccaactctgt gggctgggcc
     2041 gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac
     2101 cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc
     2161 gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc
     2221 tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac
     2281 agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc
     2341 ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga
     2401 aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc
     2461 cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc
     2521 gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac
     2581 cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc
     2641 agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcggc
     2701 aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc
     2761 gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg
     2821 gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc
     2881 atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc
     2941 tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg
     3001 cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac
     3061 gccggctaca ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc
     3121 ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg
     3181 cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg
     3241 cacgccattc tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag
     3301 atcgagaaga tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac
     3361 agcagattcg cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag
     3421 gaagtggtgg acaagggcgc ttccgcccag agcttcatcg agcggatgac caacttcgat
     3481 aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc
     3541 gtgtataacg agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc
     3601 ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg
     3661 accgtgaagc agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa
     3721 atctccggcg tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa
     3781 attatcaagg acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc
     3841 gtgctgaccc tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat
     3901 gcccacctgt tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg
     3961 ggcaggctga gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc
     4021 ctggatttcc tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac
     4081 gacagcctga cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc
     4141 ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag
     4201 acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc
     4261 gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag
     4321 agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac
     4381 cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg
     4441 cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac
     4501 gccatcgtgc ctcagagctt tctgaaggac gactccatcg acaacaaggt gctgaccaga
     4561 agcgacaaga accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg
     4621 aagaactact ggcggcagct gctgaacgcc aagctgatta cccagagaaa gttcgacaat
     4681 ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga
     4741 cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg
     4801 aacactaagt acgacgagaa tgacaagctg atccgggaag tgaaagtgat caccctgaag
     4861 tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac
     4921 aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa
     4981 aagtacccta agctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg
     5041 aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac
     5101 agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag
     5161 cggcctctga tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa gggccgggat
     5221 tttgccaccg tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa aaagaccgag
     5281 gtgcagacag gcggcttcag caaagagtct atcctgccca agaggaacag cgataagctg
     5341 atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcgacag ccccaccgtg
     5401 gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt
     5461 gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc
     5521 gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct
     5581 aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa
     5641 ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc
     5701 agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca gctgtttgtg
     5761 gaacagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt ctccaagaga
     5821 gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa gcaccgggat
     5881 aagcccatca gagagcaggc cgagaatatc atccacctgt ttaccctgac caatctggga
     5941 gcccctgccg ccttcaagta ctttgacacc accatcgacc ggaagaggta caccagcacc
     6001 aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta cgagacacgg
     6061 atcgacctgt ctcagctggg aggcgacagc cccaagaaga agagaaaggt ggaggccagc
     6121 gggccggccg gatccgggcg cgccgacgcg ctggacgatt tcgatctcga catgctgggt
     6181 tctgatgccc tcgatgactt tgacctggat atgttgggaa gcgacgcatt ggatgacttt
     6241 gatctggaca tgctcggctc cgatgctctg gacgatttcg atctcgatat gttagggtca
     6301 gacgcactgg atgatttcga ccttgatatg ttgggaagcg atgcccttga tgatttcgac
     6361 ctggacatgc tcggcagcga cgccctggac gatttcgatc tggacatgct ggggtccgat
     6421 gccttggatg attttgactt ggatatgctg gggagtgatg ccctggacga ctttgacctg
     6481 gacatgctgg gctccgatgc gctcgatgac ttcgatttgg atatgttgta tatcgattga
     6541 ttaattaaga attcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
     6601 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     6661 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     6721 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagagaata gcaggcatgc
     6781 tggggagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     6841 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     6901 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct
     6961 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     7021 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7081 agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     7141 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     7201 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     7261 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7321 caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg
     7381 ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     7441 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     7501 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     7561 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     7621 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     7681 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     7741 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     7801 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     7861 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     7921 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     7981 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     8041 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     8101 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     8161 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8221 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8281 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     8341 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     8401 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8461 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8521 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggaag ccgcggtatc
     8581 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8641 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8701 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     8761 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     8821 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tag
//
