LOCUS       sequence-179041-        8046 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  pAC154-dual-dCas9VP160-sgExpression with MYRF sg2 cloned into BbsI 
            site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Pouladi M
  TITLE     Pouladi lab MYRF plasmids 2017
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 8046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pYTP MYRF sg2
FEATURES             Location/Qualifiers
     source          1..8046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..36
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             40..60
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(70..93,94..96,97..2652,2653..2655,2656..4170)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(70..93,94..96,97..2583,2584..2586,2587..4170)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4174..4194
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4228..4611
                     /codon_start=1
                     /product="10 tandem repeats of the minimal activation 
                     domain of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP160
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGS
                     DALDDFDLDMLGSDALDDFDLDML"
     primer_bind     complement(4656..4673)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4662..4869
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4878..5018
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4878..5007
                     /label=AAV2 ITR
     rep_origin      5093..5548
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5180..5199)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5390..5411
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5565..5584)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5684..5706
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5744..5762)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5830..5934
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5935..6003,6004..6795)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6153..6172)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6966..7554
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7455..7474
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7616..7864
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7616..7636
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7787..7806
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        7885..7960
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gccaccatgt acccatacga tgttccagat tacgcttcgc cgaagaaaaa gcgcaaggtc
       61 gaagcgtccg acaagaagta cagcatcggc ctggccatcg gcaccaactc tgtgggctgg
      121 gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc
      181 gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca
      241 gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg
      301 atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc
      361 cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc
      421 ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg
      481 agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg
      541 gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac
      601 agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa
      661 aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag
      721 agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc
      781 ggcaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg
      841 gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct ggacaacctg
      901 ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac
      961 gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc
     1021 gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc
     1081 gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc
     1141 tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc
     1201 atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg
     1261 ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag
     1321 ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa
     1381 aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga
     1441 aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc
     1501 gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc
     1561 gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc
     1621 accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc
     1681 ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa
     1741 gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg
     1801 gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg
     1861 aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat
     1921 atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc
     1981 tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc
     2041 tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca
     2101 atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac
     2161 gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat
     2221 agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa gggcatcctg
     2281 cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac
     2341 atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc
     2401 gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa
     2461 caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaat
     2521 gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg
     2581 gacgccatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc
     2641 agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag
     2701 atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac
     2761 aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg cttcatcaag
     2821 agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg
     2881 atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg
     2941 aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc
     3001 aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc
     3061 aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg
     3121 cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc
     3181 tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg
     3241 aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg
     3301 gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc
     3361 gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag
     3421 ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc
     3481 gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag
     3541 agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc
     3601 atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg
     3661 cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc
     3721 gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg
     3781 gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt
     3841 gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag
     3901 agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg
     3961 gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg
     4021 ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc
     4081 accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca
     4141 cggatcgacc tgtctcagct gggaggcgac agccccaaga agaagagaaa ggtggaggcc
     4201 agcgggccgg ccggatccgg gcgcgccgac gcgctggacg atttcgatct cgacatgctg
     4261 ggttctgatg ccctcgatga ctttgacctg gatatgttgg gaagcgacgc attggatgac
     4321 tttgatctgg acatgctcgg ctccgatgct ctggacgatt tcgatctcga tatgttaggg
     4381 tcagacgcac tggatgattt cgaccttgat atgttgggaa gcgatgccct tgatgatttc
     4441 gacctggaca tgctcggcag cgacgccctg gacgatttcg atctggacat gctggggtcc
     4501 gatgccttgg atgattttga cttggatatg ctggggagtg atgccctgga cgactttgac
     4561 ctggacatgc tgggctccga tgcgctcgat gacttcgatt tggatatgtt gtatatcgat
     4621 tgattaatta agaattccta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     4681 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     4741 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     4801 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaga atagcaggca
     4861 tgctggggag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     4921 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     4981 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
     5041 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
     5101 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5161 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5221 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5281 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     5341 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5401 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
     5461 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5521 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
     5581 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     5641 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     5701 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     5761 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     5821 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5881 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     5941 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6001 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6061 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6121 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     6181 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     6241 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     6301 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     6361 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     6421 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     6481 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     6541 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     6601 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg aagccgcggt
     6661 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     6721 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     6781 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6841 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6901 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6961 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7021 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7081 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     7141 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7201 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7261 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7321 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7381 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7441 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7501 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7561 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgtgaggg
     7621 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
     7681 tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
     7741 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
     7801 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
     7861 caccgggcgc ggcgcgggcc ggcagtttta gagctagaaa tagcaagtta aaataaggct
     7921 agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttgttt tagagctaga
     7981 aatagcaagt taaaataagg ctagtccgtt tttagcgcgt gcgccaattc tgcagacaaa
     8041 tggctc
//
