LOCUS       sequence-179087-        7620 bp    DNA     circular SYN 15-APR-2025
DEFINITION  mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Schmidt K, Joyce CE, Buquicchio F, Brown A, Ritz J, Distel RJ, Yoon 
            CH, Novina CD
  TITLE     The lncRNA SLNCR1 Mediates Melanoma Invasion through a Conserved 
            SRA1-like Region.
  JOURNAL   Cell Rep. 2016 May 31;15(9):2025-37. doi: 
            10.1016/j.celrep.2016.04.018. Epub 2016 May 19.
  PUBMED    27210747
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.04.018"; journalName: "Cell Rep"; date: 
            "2016-05-31- 31"; volume: "15"; issue: "9"; pages: "2025-37"
COMMENT     Sequence Label: pCDNA3.1(-) + SLNCR1 _462-572 (conserved seque...
FEATURES             Location/Qualifiers
     source          1..7620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2977..2994)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2983..3207
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3253..3681
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3340..3359)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3550..3571
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3690..3710)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3695..4024
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3875..4010
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3937..3956
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4091..4885
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4145..4164)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4755..4774
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5059..5180
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5096..5115)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5150..5169
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5229..5245)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5229..5245)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5242..5264)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5253..5269
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5277..5283,5284..5301,5302..5307))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5322..5343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5460..5477)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5631..6219)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5711..5730)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6390..7181,7182..7250))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7013..7032
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7251..7355)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7430..7449)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacgggccc tctagactcg agcggccgcc actgtgctgg atatctgcag
      721 aattccacca cactggacta gtggatccga gctcggtacc aagcttcccg ggatacttag
      781 gatccagggc gcgcaggcgg cgcgggtgcg cggtgcggcg ctggtatcca gaggacgcgg
      841 tcaccgcctc tggcatttgt cgttctgcgc ttctccgcaa ggaccctctg ttaggcaggc
      901 gcccaccgta agcctcccgg gccttgtgaa cctgcaaacc caagtctgag agacgatccg
      961 ccttcagcgc tttccagctt ggcagagagg ctttcccggc ggggatcttt ggttggcgct
     1021 ggcgatgcgc ggggaagaaa ggcgaggagc ggcgtccagg ctgggtgatg tcccagcacg
     1081 agtaggcggg atgcgctcgc ttggtcctcc gggcgcccgg tccctgcccg cgtcgcgcgc
     1141 ccacccctgg ggacgagaag gcggccgcct gaggaccccc gcccgcgacc tccgcgagtc
     1201 tggagcgcag aggacagggt ctggctgctc tttggccttg gatggactgc ttaacagctg
     1261 ggtcgccaag ctggaggtat ttttccctct ccaccctggt cttctcctgt aacgtgtggc
     1321 cgccttttcc agcacggcct cctgccttcc tggtgcactt tttggagaac gtggtggaat
     1381 cagaggtttc tggctgactc ggtgggtgct ttgaaccagg aaaggacaag aaagaggatg
     1441 ggaaggactg atccacattc ccaccaggaa gtttagcaga acccccgcgt gccacctgga
     1501 ccccttggaa ggacctggct caggctggac cacctcttga gaggcaggag ctctggattt
     1561 gatcaagaat tctttgctga gcatggtgcc tcatgcctat aatcccaaca ctttgggagg
     1621 ccagtgtggg aggatctctt gagcccagga gttcaagact agcctgggca acacagagag
     1681 accccatctc taaaataata ataataataa aataaaaaat tagcagggca tggtggcatg
     1741 tgcctgtagt cccagctacc caggaggctg aggcaagagg atggctggag cctgggatgt
     1801 tgaggctgca atgaactgtg attaccccac tgcactccag cctgggcaaa agagcgagag
     1861 accctgtctc aaataataat aataataata atcttatttt ggagaataaa gagacctctg
     1921 gatttgaggt gccatttggg tagaaagaaa agacgtttac accgagaaat agtctgtgtt
     1981 gccctgaagg agcagaggga tgcatcgctg gaggtgacct acagttgaag aagactcatt
     2041 atgacagacc ttgtccttct tccttgtgga aagtgtttcc tctgctgcta ctgctcatga
     2101 gactcttccc cctccctgtc ccagggaacc aaagggcttt ctaccacacc ctttcttgcc
     2161 ccccgcctcc catgtctgct gtgcctttgt actcagcaat tcttgtttgc tccattatct
     2221 tccagccgga tacagagtga atagttaacc acacttaggt caaataggat ctaaattttt
     2281 gttcctgctc cgtgtaaaga ggccagtgtt tgtgtgttgc aagcagcctt ggaatagtaa
     2341 ctcttctcat ttgtttggga tctggccacc aagttccaga atgatacacg gatcagtgca
     2401 gaagttcatc aggctctcgg accttagggc tgttggagaa ggcttcagca gcagaactga
     2461 tggtgaaggc tcgtgttctc catcctcaac tttctttgct tcgatcatac acaagaatac
     2521 atttggaagg gcaaaaaatg aacactgtcg ttcattgcag ccgtgttttg tgacacagat
     2581 gcacagtctg ctgtgaagac cttctctcaa gtggcatttg ggagtccatg ccagatcatg
     2641 gtgcttcatg agagactgac agctatcagg ggttgtggca cttagtgagg actctcctcc
     2701 cccagtgtgt gctgatgaca catacacacc tgacaatagc ttgagtcttc tctgttcctt
     2761 ttactctgta gccaacatac acatgattta aaaccctttc taaatatcta tcatggttca
     2821 tccttgtcca aatgcagagt cagagctatt tgtacttcat tattatttcc aaggcgaata
     2881 gttggctttc tttttgcaaa aataattaaa gtttttgtat gttgcagttg ctctagataa
     2941 gtatcacgtg aagcttaagt ttaaaccgct gatcagcctc gactgtgcct tctagttgcc
     3001 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     3061 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     3121 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     3181 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     3241 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     3301 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     3361 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     3421 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     3481 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     3541 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     3601 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     3661 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     3721 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     3781 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3841 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3901 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3961 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     4021 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     4081 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     4141 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     4201 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     4261 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     4321 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     4381 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     4441 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     4501 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     4561 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     4621 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     4681 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     4741 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     4801 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     4861 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     4921 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     4981 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     5041 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5101 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5161 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     5221 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     5281 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     5341 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     5401 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     5461 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5521 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5581 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5641 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5701 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5761 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5821 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5881 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5941 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6001 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6061 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6121 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6181 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6241 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6301 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6361 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6421 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6481 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6541 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6601 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6661 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6721 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6781 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6841 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6901 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6961 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7021 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7081 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7141 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7201 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7261 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7321 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7381 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     7441 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     7501 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     7561 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
//
