LOCUS       sequence-179757-        8076 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  pAC154-dual-dCas9VP160-sgExpression with MYRF sg5 cloned into BbsI 
            site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Pouladi M
  TITLE     Pouladi lab MYRF plasmids 2017
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 8076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pYTP MYRF sg5
FEATURES             Location/Qualifiers
     source          1..8076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..56
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             60..80
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(90..113,114..116,117..2672,2673..2675,2676..4190)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(90..113,114..116,117..2603,2604..2606,2607..4190)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4194..4214
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4248..4631
                     /codon_start=1
                     /product="10 tandem repeats of the minimal activation 
                     domain of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP160
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGS
                     DALDDFDLDMLGSDALDDFDLDML"
     primer_bind     complement(4676..4693)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4682..4889
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4898..5038
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4898..5027
                     /label=AAV2 ITR
     rep_origin      5113..5568
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5200..5219)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5410..5431
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5585..5604)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5704..5726
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5764..5782)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5850..5954
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5955..6023,6024..6815)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6173..6192)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6986..7574
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7475..7494
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7636..7884
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7636..7656
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7807..7826
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        7905..7980
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ttttttcagg ttggaccggt gccaccatgt acccatacga tgttccagat tacgcttcgc
       61 cgaagaaaaa gcgcaaggtc gaagcgtccg acaagaagta cagcatcggc ctggccatcg
      121 gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat
      181 tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggagccctgc
      241 tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat
      301 acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca
      361 aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga
      421 agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt
      481 accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc
      541 ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg
      601 gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct
      661 acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc
      721 tgtctgccag actgagcaag agcagacggc tggaaaatct gatcgcccag ctgcccggcg
      781 agaagaagaa tggcctgttc ggcaacctga ttgccctgag cctgggcctg acccccaact
      841 tcaagagcaa cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg
      901 acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg
      961 ccgccaagaa cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga
     1021 tcaccaaggc ccccctgagc gcctctatga tcaagagata cgacgagcac caccaggacc
     1081 tgaccctgct gaaagctctc gtgcggcagc agctgcctga gaagtacaaa gagattttct
     1141 tcgaccagag caagaacggc tacgccggct acattgacgg cggagccagc caggaagagt
     1201 tctacaagtt catcaagccc atcctggaaa agatggacgg caccgaggaa ctgctcgtga
     1261 agctgaacag agaggacctg ctgcggaagc agcggacctt cgacaacggc agcatccccc
     1321 accagatcca cctgggagag ctgcacgcca ttctgcggcg gcaggaagat ttttacccat
     1381 tcctgaagga caaccgggaa aagatcgaga agatcctgac cttccgcatc ccctactacg
     1441 tgggccctct ggccagggga aacagcagat tcgcctggat gaccagaaag agcgaggaaa
     1501 ccatcacccc ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc cagagcttca
     1561 tcgagcggat gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca
     1621 gcctgctgta cgagtacttc accgtgtata acgagctgac caaagtgaaa tacgtgaccg
     1681 agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc
     1741 tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa
     1801 tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg
     1861 gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa
     1921 acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga
     1981 tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga
     2041 agcggcggag atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg
     2101 acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa
     2161 acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc
     2221 aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg
     2281 ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg
     2341 gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga
     2401 agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg
     2461 gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt
     2521 acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc
     2581 ggctgtccga ctacgatgtg gacgccatcg tgcctcagag ctttctgaag gacgactcca
     2641 tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg caagagcgac aacgtgccct
     2701 ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac gccaagctga
     2761 ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg
     2821 ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca aagcacgtgg
     2881 cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag ctgatccggg
     2941 aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt
     3001 tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg
     3061 tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg
     3121 actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg
     3181 ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc
     3241 tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa accggggaga
     3301 tcgtgtggga taagggccgg gattttgcca ccgtgcggaa agtgctgagc atgccccaag
     3361 tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc
     3421 ccaagaggaa cagcgataag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg
     3481 gcggcttcga cagccccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg
     3541 gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa
     3601 gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa
     3661 aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga
     3721 gaatgctggc ctctgccggc gaactgcaga agggaaacga actggccctg ccctccaaat
     3781 atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata
     3841 atgagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag atcatcgagc
     3901 agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac aaagtgctgt
     3961 ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat atcatccacc
     4021 tgtttaccct gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg
     4081 accggaagag gtacaccagc accaaagagg tgctggacgc caccctgatc caccagagca
     4141 tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggcgac agccccaaga
     4201 agaagagaaa ggtggaggcc agcgggccgg ccggatccgg gcgcgccgac gcgctggacg
     4261 atttcgatct cgacatgctg ggttctgatg ccctcgatga ctttgacctg gatatgttgg
     4321 gaagcgacgc attggatgac tttgatctgg acatgctcgg ctccgatgct ctggacgatt
     4381 tcgatctcga tatgttaggg tcagacgcac tggatgattt cgaccttgat atgttgggaa
     4441 gcgatgccct tgatgatttc gacctggaca tgctcggcag cgacgccctg gacgatttcg
     4501 atctggacat gctggggtcc gatgccttgg atgattttga cttggatatg ctggggagtg
     4561 atgccctgga cgactttgac ctggacatgc tgggctccga tgcgctcgat gacttcgatt
     4621 tggatatgtt gtatatcgat tgattaatta agaattccta gagctcgctg atcagcctcg
     4681 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     4741 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     4801 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4861 tgggaagaga atagcaggca tgctggggag cggccgcagg aacccctagt gatggagttg
     4921 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     4981 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
     5041 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     5101 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     5161 gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt
     5221 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     5281 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     5341 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     5401 tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt
     5461 cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt
     5521 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     5581 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     5641 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     5701 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     5761 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     5821 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5881 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5941 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6001 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6061 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6121 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6181 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6241 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6301 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6361 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6421 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6481 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6541 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6601 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6661 gtgagcgtgg aagccgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6721 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6781 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6841 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6901 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6961 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7021 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7081 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7141 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7201 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7261 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7321 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7381 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7441 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7501 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7561 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7621 cttttgctca catgtgaggg cctatttccc atgattcctt catatttgca tatacgatac
     7681 aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa
     7741 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
     7801 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
     7861 atcttgtgga aaggacgaaa caccgccctg tgccggtggt gggcgtttta gagctagaaa
     7921 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
     7981 ttttttgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt tttagcgcgt
     8041 gcgccaattc tgcagacaaa tggctctaga ggtacc
//
