LOCUS       sequence-18007-e         773 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  TITLE     Collier lab plasmids
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET-15b EF WT
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..84
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gtttaacttt aagaaggaga tataccatgg ggcagcagcc atcatcatca tcatcacagc
       61 agcggcctgg tgccgcgcgg cagccatatg aatgaacatt acactgagag tgatattaaa
      121 agaaaccata aaactgaaaa aaataaaact gaaaaagaaa aatttaaaga cagtattaat
      181 aacttagtta aaacagaatt taccaatgaa actttagata aaatacagca gacacaagac
      241 ttattaaaaa agatacctaa ggatgtactt gaaatttata gtgaattagg aggagaaatc
      301 tattttacag atatagattt agtagaacat aaggagttac aagatttaag tgaagaagag
      361 aaaaatagta tgaatagtag aggtgaaaaa gttccgtttg catcccgttt tgtatttgaa
      421 aagaaaaggg aaacacctaa attaattata aatatcaaag attatgcaat taatagtgaa
      481 caaagtaaag aagtatatta tgaaattgga aaggggattt ctcttgatat tataagtaag
      541 gataaatctc tagatccaga gtttttaaat ttaattaaga gtttaagcga tgatagtgat
      601 agtagcgacc ttttatttag tcaaaaattt aaagagaagc tagaattgaa taataaaagt
      661 atagatataa attttataaa agaaaattta actgaatttc agcatgcgtt ttctttagcg
      721 ttttcttatt attttgcacc tgaccataga acggtattag agttatatgc ccc
//
