LOCUS       sequence-180165-        9598 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Translation reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Yan X, Hoek TA, Vale RD, Tanenbaum ME
  TITLE     Dynamics of Translation of Single mRNA Molecules In Vivo.
  JOURNAL   Cell. 2016 May 5;165(4):976-89. doi: 10.1016/j.cell.2016.04.034.
  PUBMED    27153498
REFERENCE   2  (bases 1 to 9598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.04"; journalName: "Cell"; date: "2016-05-5- 5";
            volume: "165"; issue: "4"; pages: "976-89"
COMMENT     Sequence Label: pcDNA4TO-5xGCN4_v4-kif18b-24xPP7
FEATURES             Location/Qualifiers
     source          1..9598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      984..993
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1038..1094
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1110..1166
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1182..1238
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1254..1310
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1326..1382
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(5606..5623)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5612..5836
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5882..6310
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5969..5988)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6179..6200
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6319..6339)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6324..6653
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6504..6639
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6566..6585
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6701..6748
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6767..7141
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7271..7392
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7308..7327)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7362..7381
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7441..7457)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7441..7457)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7454..7476)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7465..7481
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7489..7495,7496..7513,7514..7519))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7534..7555
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7672..7689)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7843..8431)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7923..7942)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8602..9393,9394..9462))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9225..9244
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9463..9567)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaaactaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttgccacca tgggcgccat catacacgcg gccgcaatca
     1021 tcggtccggg ttctggagaa gagctactat ccaagaatta tcatcttgag aacgaggtag
     1081 cgcgtttgaa gaagggttcc ggctcaggag aggaactgct ctcgaagaac tatcatcttg
     1141 aaaatgaggt cgctcgatta aaaaagggat cgggcagtgg tgaggaacta ctttcaaaga
     1201 attaccacct cgaaaacgaa gtagctcgat taaagaaagg ttcagggtcg ggtgaagaat
     1261 tactgagtaa aaattatcat ctggaaaatg aggtagcgag actaaaaaag gggagtggtt
     1321 ctggcgagga attgctatcg aaaaattatc atcttgagaa cgaagttgct aggctcaaaa
     1381 agggctcagg ctcaggcacc gcggtaaaca tatggatcga tcagcggccg caccggtcag
     1441 cagtggagga cagcacgctg caagtagtgg tacgggtgcg gccccccacc cctcgggagc
     1501 tggacagtca gcggcggcca gtggttcagg tggtggacga gcgggtgctg gtgtttaacc
     1561 ctgaggagcc cgatggaggg ttccctggcc tgaaatgggg tggcacccat gatggcccca
     1621 agaagaaggg caaagacctg acgtttgtct ttgaccgggt ctttggcgag gcggccaccc
     1681 aacaggacgt gttccagcac accacgcaca gcgtcctgga cagcttcctc cagggctaca
     1741 actgctcagt gtttgcctac ggggccaccg gggctgggaa gacacacacc atgctgggaa
     1801 gggaggggga ccccggcatc atgtacctga ccaccgtgga actgtacagg cgcctggagg
     1861 cccgccagca ggagaagcac ttcgaggtgc tcatcagcta ccaggaggtg tataatgaac
     1921 agatccatga cctcctggag cccaaggggc cccttgccat ccgcgaggac cccgacaagg
     1981 gggtggtggt gcaaggactt tctttccacc agccagcctc agccgagcag ctgctggaga
     2041 tactgaccag ggggaaccgt aaccgcacgc agcaccccac tgatgccaac gcgacttcct
     2101 cccgctccca tgccatcttc cagatctttg tgaagcagca ggaccgggtt ccaggactga
     2161 cccaggctgt ccaggtggcc aagatgagcc tgattgacct ggctggctca gagcgggcat
     2221 ccagcaccca tgcgaagggg gagcggctgc gggagggggc caacatcaac cgctctctgc
     2281 tggcgctcat caacgtcctc aatgccttgg ccgatgcaaa gggccgcaag accgctgtgc
     2341 cctacgcgga cagcgcactg acccgcctgc tcaaagactc cctcgggggc aactgccgca
     2401 cagtgatgat cgctgccatc agcccctcca gcctgaccta cgaggacacg tataataccc
     2461 tcaaatatgc cgaccgggcc aaggagatca ggctctcgct gaagagcaat gtgaccagcc
     2521 tggactgtca catcagccag tatgctacca tctgccaaca gctccaggct gaggtagccg
     2581 ctctgaggaa gaagctccaa gtgtatgagg ggggaggcca gcccccacca caggacctcc
     2641 caggatctcc caagtcggga ccaccaccag aacaccttcc cagctccccc ttgccacccc
     2701 accctcccag ccagccctgc accccagagc tccctgcagg gcctagagcc cttcaagagg
     2761 agagtctggg gatggaggcc caggtggaga gggccatgga agggaactct tcagaccagg
     2821 agcagtcccc agaggatgag gatgaaggcc cagctgagga ggttccaacc cagatgccag
     2881 agcagaaccc cacacatgca ctgccagagt cccctcgcct gaccctgcag cccaagccag
     2941 tcgtgggcca cttctcagca cgggaactgg atggggaccg ttctaagcgg ttggccctaa
     3001 aggtgctgtg cgttgcccag cggcagtact ccctgctcca agcagccaac ctcctgacgc
     3061 ccgacatgat cacagagttt gagaccctac agcagctggt gcaagaggaa aaaattgagc
     3121 ctggggcaga ggccttgagg acttcaggcc tggccagggg ggcacctctg gctcaggagc
     3181 tgtgttcaga gtcaaagcct ccaggataca ctggccctgt gacccggact atggcgaggc
     3241 gactgagtgg ccccctgcac accctgggaa tcccgcctgg acccaactgc accccagccc
     3301 aggggtcccg atggcccatg gagaagaaga ggaggagacc aagcgccttg gaggcagaca
     3361 gtcccatggc cccaaagcgg ggcaccaagc gccagcgcca gtccttcctg ccctgcctaa
     3421 ggagagggtc tctgcctgac acccaacctt cacaggggcc cagcaccccc aaaggagaaa
     3481 gggcctcctc cccctgccat tcccctcgcg tttgcccagc cacagtcatc aaaagccggg
     3541 tgcccctggg cccttccgcc atgcagaact gctccacccc gctggctctg cccactcgag
     3601 acctcaatgc cacctttgat ctctctgagg agcctccctc aaagcccagt ttccatgaat
     3661 gcattggctg ggacaaaata ccccaggagc tgagcaggct ggaccagccc ttcatcccca
     3721 gggcacctgt gcccctgttc accatgaagg gccccaagcc aacatcttcc ctccctggga
     3781 cctctgcctg caagaagaag cgcgttgcga gttcctcagt ctcccatggc cgcagccgca
     3841 tcgcccgcct ccccagcagc actttgaaga ggccagctgg gccccttgta ctcccagagc
     3901 tgcccttgag tcccctgtgc cctagcaacc ggaggaatgg aaaggacctc atcagggtgg
     3961 ggagagcact ctcagcaggg aacggcgtca ccaaggtgtc cgatatctaa cgacacgatt
     4021 cgaagatcgc atcgctagcc cggttgtaca ggtaccggat cctaaggtac ctaattgcct
     4081 agaaaggagc agacgatatg gcgtcgctcc ctgcaggtcg actctagaaa ccagcagagc
     4141 atatgggctc gctggctgca gtattcccgg gttcattaga tcctaaggta cctaattgcc
     4201 tagaaaggag cagacgatat ggcgtcgctc cctgcaggtc gactctagaa accagcagag
     4261 catatgggct cgctggctgc agtattcccg ggttcattag atcctaaggt acctaattgc
     4321 ctagaaagga gcagacgata tggcgtcgct ccctgcaggt cgactctaga aaccagcaga
     4381 gcatatgggc tcgctggctg cagtattccc gggttcatta gatcctaagg tacctaattg
     4441 cctagaaagg agcagacgat atggcgtcgc tccctgcagg tcgactctag aaaccagcag
     4501 agcatatggg ctcgctggct gcagtattcc cgggttcatt agatcctaag gtacctaatt
     4561 gcctagaaag gagcagacga tatggcgtcg ctccctgcag gtcgactcta gaaaccagca
     4621 gagcatatgg gctcgctggc tgcagtattc ccgggttcat tagatcctaa ggtacctaat
     4681 tgcctagaaa ggagcagacg atatggcgtc gctccctgca ggtcgactct agaaaccagc
     4741 agagcatatg ggctcgctgg ctgcagtatt cccgggttca ttagatccta aggtacctaa
     4801 ttgcctagaa aggagcagac gatatggcgt cgctccctgc aggtcgactc tagaaaccag
     4861 cagagcatat gggctcgctg gctgcagtat tcccgggttc attagatcct aaggtaccta
     4921 attgcctaga aaggagcaga cgatatggcg tcgctccctg caggtcgact ctagaaacca
     4981 gcagagcata tgggctcgct ggctgcagta ttcccgggtt cattagatcc taaggtacct
     5041 aattgcctag aaaggagcag acgatatggc gtcgctccct gcaggtcgac tctagaaacc
     5101 agcagagcat atgggctcgc tggctgcagt attcccgggt tcattagatc ctaaggtacc
     5161 taattgccta gaaaggagca gacgatatgg cgtcgctccc tgcaggtcga ctctagaaac
     5221 cagcagagca tatgggctcg ctggctgcag tattcccggg ttcattagat cctaaggtac
     5281 ctaattgcct agaaaggagc agacgatatg gcgtcgctcc ctgcaggtcg actctagaaa
     5341 ccagcagagc atatgggctc gctggctgca gtattcccgg gttcattaga tcctaaggta
     5401 cctaattgcc tagaaaggag cagacgatat ggcgtcgctc cctgcaggtc gactctagaa
     5461 accagcagag catatgggct cgctggctgc agtattcccg ggttcattag atctcgcgaa
     5521 gggcgaattc acgaggcgcg cccggatcgc gatcgcaatc ttaattaact cgagtctaga
     5581 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     5641 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     5701 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     5761 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     5821 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     5881 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5941 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6001 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6061 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     6121 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     6181 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6241 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6301 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     6361 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     6421 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     6481 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     6541 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     6601 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     6661 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     6721 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
     6781 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
     6841 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
     6901 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
     6961 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
     7021 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
     7081 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
     7141 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     7201 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     7261 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7321 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7381 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     7441 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7501 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7561 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7621 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7681 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7741 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7801 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7861 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7921 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7981 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8041 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8101 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8161 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8221 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8281 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8341 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8401 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8461 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8521 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8581 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8641 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8701 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8761 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8821 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8881 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8941 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9001 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9061 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9121 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9181 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9241 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9301 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9361 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9421 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9481 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9541 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
