LOCUS       sequence-180290-        7252 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expression of HCV E1E2 genes cloned from patient serum. United 
            Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone 
            number).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Urbanowicz RA, McClure CP, Brown RJ, Tsoleridis T, Persson MA, Krey 
            T, Irving WL, Ball JK, Tarr AW
  TITLE     A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in 
            Vaccine Research Reveals Extremes of Monoclonal Antibody 
            Neutralization Resistance.
  JOURNAL   J Virol. 2015 Dec 23;90(7):3288-301. doi: 10.1128/JVI.02700-15.
  PUBMED    26699643
REFERENCE   2  (bases 1 to 7252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-12-23- 23"; volume: "90"; issue:
            "7"; pages: "3288-301"
COMMENT     Sequence Label: UKNP5.1.1
FEATURES             Location/Qualifiers
     source          1..7252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1815..1856
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1866..1883
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1906..1923)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1912..2136
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2182..2610
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2269..2288)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2479..2500
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2619..2639)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2624..2954
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2805..2940
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2867..2886
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3021..3815
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3075..3094)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3685..3704
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3991..4112
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4028..4047)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4082..4101
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4161..4177)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4161..4177)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4174..4196)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4185..4201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4209..4215,4216..4233,4234..4239))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4254..4275
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4392..4409)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4563..5151)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4643..4662)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5322..6113,6114..6182))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5945..5964
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6183..6287)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6362..6381)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6553..6932
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6933..7136
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7087..7107
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7181..7200
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7181..7199
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 caccatgggt tgctctttct ctatctttat ccttgcactt ctctcgtgcc tgaccgtccc
       61 agcctctgca gtcccctacc ggaatgcctc tgggatctac cacgtcacca atgattgccc
      121 aaactcttcc atagtatatg aggcagataa cctgatttta cacgcacccg gctgcgtgcc
      181 ctgtgttagg gagggtaatg tgagtaggtg ctgggtccaa atcaccccca cactgtcggc
      241 cccaaacctc ggagcggtca cggctcctct tcggagagcc gttgactact tagcgggagg
      301 ggccgccctt tgctccgcgt tatacgtcgg ggacgcgtgc ggggcggtgt tcttggtagg
      361 ccaaatgttc acctataggc ctcgccagca tactacggtg caggactgca actgttctat
      421 ttacagtggc catgttagcg gccaccggat ggcatgggac atgatgatga attggtcacc
      481 tacatcggcc ttgctggtag cccagatgct acggatccct caagtggtca tggacatcat
      541 agccgggggc cactgggggg tcttgttcgc cgccgcgtac tttgcatcag cggctaactg
      601 ggctaaggtt gtgctggttc tgttcttgtt tgcgggagtt gacggtaaca cccagactgt
      661 gggcggttct gctgcttatt ctacatcagg cctggcctcg ctcttcagcc ccgggccaaa
      721 gcagaacctc cagctcatac aaaatgggag cagctggcac attaacagga ctgcccttaa
      781 ctgtaatgac agcctcaaga ctgggttcat agccggcctc atgtacgtct ataagttcaa
      841 ctccaccggg tgtccggagc ggatggctag ttgtagaccc ctcacagcct tcgaccaggg
      901 ctggggaact atcagctatg ccaacatatc gggccctagt gacgacaaac catattgctg
      961 gcactatccc ccacgaccgt gcggagtggt gccagcccga gaggtctgcg gtccagtcta
     1021 ctgtttcaca cctagcccag tggtcgttgg caccactgat cgcaaaggat atcccactta
     1081 caactggggt gaaaatgaca ctgacgtcct tctcttgaac aacacgaggc ctcccactgg
     1141 caattggttt ggctgcacct ggatgaacgc ctcagggttt gtgaagactt gcggagcgcc
     1201 accttgcatc ttagggccga caggcaacaa cagtcttaag tgccctacag attgcttccg
     1261 caagcacccg gacgccacct acaccaggtg cgggtcagga ccctggctta cgccccggtg
     1321 tttggtggac tacccttacc ggttgtggca ttacccgtgc accgtaaatt ataccatctt
     1381 taaggtgcgc atgttcattg ggggcctaga gcacaggttt gatgcggcat gtaactggac
     1441 ccgaggtgaa cgatgcgacc tcgatgatag ggatagggcc gagctaagcc cgcttctaca
     1501 cactaccaca cagtgggcca tattgccgtg ctcttttaca cccacgcctg ctcttagtac
     1561 tggcctcatt cacttacacc agaatatagt agacacccag tatctttacg gcctgagctc
     1621 cagcatcgtc tcgtgggccg ttaagtggga gtacatagtg ctggccttcc tgctacttgc
     1681 tgatgcccgt gtctgtactt gcctatggat catgctccta gtctgtcagg ctgaagcata
     1741 aaaagggtca agacaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg
     1801 gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta
     1861 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
     1921 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     1981 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     2041 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     2101 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     2161 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     2221 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     2281 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca
     2341 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     2401 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     2461 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     2521 cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg
     2581 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     2641 tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt
     2701 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     2761 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     2821 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     2881 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     2941 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
     3001 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     3061 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     3121 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     3181 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     3241 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     3301 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     3361 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     3421 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     3481 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     3541 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     3601 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     3661 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     3721 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     3781 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgcgaaatg
     3841 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     3901 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     3961 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     4021 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4081 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     4141 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     4201 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     4261 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     4321 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     4381 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     4441 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     4501 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     4561 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     4621 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4681 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4741 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4801 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4861 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4921 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4981 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     5041 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     5101 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     5161 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     5221 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     5281 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     5341 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     5401 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     5461 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     5521 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     5581 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     5641 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     5701 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     5761 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     5821 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     5881 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     5941 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     6001 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     6061 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     6121 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     6181 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     6241 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     6301 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tcgactctca
     6361 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     6421 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     6481 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     6541 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     6601 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     6661 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     6721 cgccaatagg gactttccat tgacgtcaat gggtggacta tttacggtaa actgcccact
     6781 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     6841 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     6901 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     6961 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     7021 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     7081 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct
     7141 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
     7201 agacccaagc tggctagtta agcttggtac cgagctcgga tccagtaccc tt
//
