LOCUS       sequence-180325-        8816 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector with dox inducible expression of shRNA targeting 
            human FOXM1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  TITLE     FOXM1 toolbox
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Tet-pLKO-FOXM1-shRNA-#3
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(103..702)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(186..206)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     683..702
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    722..1295
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(882..901)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     1111..1128
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(1330..1950)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     1858..1877
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        complement(1961..1979)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2007..2026)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          2034..2606
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     promoter        complement(2615..3125)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2678..2696)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    3174..3291
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(3392..3486)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    3392..3410
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3420..3438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(3456..3475)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     CDS             complement(3775..3819)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4004..4237
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4730..4855
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4902..5082
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5083..5309)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(5337..5357)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5337..5355)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5376..5392)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5376..5392)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5389..5411)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5400..5416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5424..5430,5431..5448,5449..5454))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5469..5490
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5607..5624)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5778..6366)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5858..5877)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6537..7328,7329..7397))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7160..7179
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7398..7502)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7528..7983)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7665..7686)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7877..7896
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8110..8132
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8124..8141
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8125..8141
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8151..8169
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      8190..8325
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8365..8486
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8376..8395)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8430..8449
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             8558..8791
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 gctaagatct acagctgcct tgtaagtcat tggtcttaaa ggtaccgatg catggggtcg
       61 tgcgctcctt tcggtcgggc gctgcgggtc gtggggcggg cgtcaggcac cgggcttgcg
      121 ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg tcggcggtga cggtgaagcc
      181 gagccgctcg tagaagggga ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc
      241 ggcgcgctcg gccgcctcca ctccggggag cacgacggcg ctgcccagac ccttgccctg
      301 gtggtcgggc gagacgccga cggtggccag gaaccacgcg ggctccttgg gccggtgcgg
      361 cgccaggagg ccttccatct gttgctgcgc ggccagccgg gaaccgctca actcggccat
      421 gcgcgggccg atctcggcga acaccgcccc cgcttcgacg ctctccggcg tggtccagac
      481 cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt
      541 gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg cggtccggat cgacggtgtg
      601 gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc tggggacgtc
      661 gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc atggtaagct ccggtggatc
      721 cttatcatcg tgtttttcaa aggaaaacca cgtccccgtg gttcgggggg cctagacgtt
      781 tttttaacct cgactaaaca catgtaaagc atgtgcaccg aggccccaga tcagatccca
      841 tacaatgggg taccttctgg gcatccttca gccccttgtt gaatacgctt gaggagagcc
      901 atttgactct ttccacaact atccaactca caacgtggca ctggggttgt gccgcctttg
      961 caggtgtatc ttatacacgt ggcttttggc cgcagaggca cctgtcgcca ggtggggggt
     1021 tccgctgcct gcaaagggtc gctacagacg ttgtttgtct tcaagaagct tccagaggaa
     1081 ctgcttcctt cacgacattc aacagacctt gcattccttt ggcgagaggg gaaagacccc
     1141 taggaatgct cgtcaagaag acagggccag gtttccgggc cctcacattg ccaaaagacg
     1201 gcaatatggt ggaaaataac atatagacaa acgcacaccg gccttattcc aagcggcttc
     1261 ggccagtaac gttagggggg ggggagggag aggggcagat ccctagaact cccgggagcc
     1321 gctgtacgcg gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
     1381 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
     1441 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
     1501 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
     1561 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
     1621 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
     1681 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
     1741 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
     1801 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
     1861 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
     1921 aatcacttta cttttatcta atctagacat atcaattcgc cctatagtga gtcgtattac
     1981 aattctttgc caaaatgatg agacagcaca ataaccagca cgttgcccag gagctgtagg
     2041 aaaaagaaga aggcatgaac atggttagca gaggggcccg gtttggactc agagtatttt
     2101 atcctcatct caaacagtgt atatcattgt aaccataaag agaaaggcag gatgatgacc
     2161 aggatgtagt tgtttctacc aataagaata tttccacgcc agccagaatt tatatgcaga
     2221 aatattctac cttatcattt aattataaca attgttctct aaaactgtgc tgaagtacaa
     2281 tataatatac cctgattgcc ttgaaaaaaa agtgattaga gaaagtactt acaatctgac
     2341 aaataaacaa aagtgaattt aaaaattcgt tacaaatgca agctaaagtt taacgaaaaa
     2401 gttacagaaa atgaaaagaa aataagagga gacaatggtt gtcaacagag tagaaagtga
     2461 aagaaacaaa attatcatga gggtccatgg tgatacaagg gacatcttcc cattctaaac
     2521 aacaccctga aaactttgcc ccctccatat aacatgaatt ttacaatagc gaaaaagaaa
     2581 gaacaatcaa gggtccccaa actcacagat ccccctgggg agagaggtcg gtgattcggt
     2641 caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg gaacaccgcg
     2701 cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc gccccttccc
     2761 ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc catcgcggtc
     2821 ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg agacgtgcgg
     2881 cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga cttaggggcg
     2941 gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg gtgacgctgc
     3001 gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc ccggaaccac
     3061 gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg cgcaacccca
     3121 accccctcga gccgcggcca aagtggatct ctgctgtccc tgtaataaac ccgaaaattt
     3181 tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct
     3241 actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg
     3301 aatactgcca tttgtctcga ggtcgagaat tcaaaaagga aatgcttgtg attcaacact
     3361 cgagtgttga atcacaagca tttccccggt gtctctatca ctgataggga acttataagt
     3421 ctctatcact gatagggatt tcacgtttat ggtgatttcc cagaacacat agcgacatgc
     3481 aaatattgcg gccgctcgag gctagtctcg tgatcgatac cgtcgagatc cgttcactaa
     3541 tcgaatggat ctgtctctgt ctctctctcc accttcttct tctattcctt cgggcctgtc
     3601 gggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac
     3661 tctattcact atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat
     3721 cattatgaat aattttatat accacagcca atttgttatg ttaaaccaat tccacaaact
     3781 tgcccattta tctaattcca ataattcttg ttcattcttt tcttgctggt tttgcgattc
     3841 ttcaattaag gagtgtatta agcttgtgta attgttaatt tctctgtccc actccatcca
     3901 ggtcgtgtga ttccaaatct gttccagaga tttattactc caactagcat tccaaggcac
     3961 agcagtggtg caaatgagtt ttccagagca accccaaatc cccaggagct gttgatcctt
     4021 taggtatctt tccacagcca ggattcttgc ctggagctgc ttgatgcccc agactgtgag
     4081 ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct gctgcactat
     4141 accagacaat aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct
     4201 tcctgctgct cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct
     4261 ctgcaccact cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac
     4321 tttatattta tataattcac ttctccaatt gtccctcata tctcctcctc caggtctgaa
     4381 gatcagcggc cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc tctatcttgt
     4441 ctaaagcttc cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta
     4501 ctgtattata taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc
     4561 ccagtatttg tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt
     4621 ctagctccct gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc
     4681 ttaaccgaat tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc
     4741 acccatctct ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc
     4801 gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct
     4861 ctggtttccc tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac
     4921 tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac
     4981 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc
     5041 agagagctcc caggctcaga tctggtctaa ccagagagac ccgtttatgt atcgagctag
     5101 gcacttaaat acaatatctc tgcaatgcgg caattcagtg gttcgtccaa tccatgtcag
     5161 acccgtctgt tgccttccta ataaggcacg atcgtaccac cttacttcca ccaatcggca
     5221 tgcacggtgc tttttctctc cttgtaaggc atgttgctaa ctcatcgtta ccatgttgca
     5281 agactacaag agtattgcat aagactacat taagcttgca gctccagctt ttgttccctt
     5341 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     5401 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     5461 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     5521 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     5581 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5641 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     5701 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     5761 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     5821 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5881 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5941 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6001 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6061 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6121 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6181 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     6241 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6301 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6361 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6421 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     6481 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     6541 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     6601 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     6661 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     6721 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     6781 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     6841 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     6901 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     6961 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7021 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     7081 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7141 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7201 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     7261 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     7321 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     7381 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     7441 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7501 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     7561 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     7621 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     7681 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     7741 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     7801 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     7861 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     7921 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     7981 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     8041 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     8101 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
     8161 cactataggg cgaattgggt acgtccctag gcctccaaaa aagcctcctc actacttctg
     8221 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     8281 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     8341 cgggatagct agagccagac atgataagat acattgatga gtttggacaa accacaacta
     8401 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     8461 ccattataag ctgcaataaa caagttcctc tcactctctg atattcattt ctttgcaagt
     8521 tataaatact gaataataag atgacatgaa ctactactgc tagagatttt ccacactgac
     8581 taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc acacactact
     8641 tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag
     8701 agctcccagg ctcagatctg gtctaaccag agagacccag tacaagcaaa aagcagatct
     8761 tgtcttcgtt gggagtgaat tagcccttcc agtcccccct tttcttttaa aaagtg
//
