LOCUS       sequence-180346-        1353 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  caspase-9 BRET Sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   den Hamer A, Dierickx P, Arts R, de Vries JSPM, Brunsveld L, Merkx M
  TITLE     Bright Bioluminescent BRET Sensor Proteins for Measuring 
            Intracellular Caspase Activity.
  JOURNAL   ACS Sens. 2017 Jun 23;2(6):729-734. doi: 10.1021/acssensors.7b00239.
            Epub 2017 May 31.
  PUBMED    28670623
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssensors.7b00239"; journalName: "ACS Sens"; date: 
            "2017-06-23- 23"; volume: "2"; issue: "6"; pages: "729-734"
COMMENT     Sequence Label: pcDNA3.1-mNeonGreen-LEHD-NanoLuc
FEATURES             Location/Qualifiers
     source          1..1353
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             25..42
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..69
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     regulatory      328..337
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aagcttgcca ccatgggaag ttcacatcat catcatcatc actcatcagg actggtgcca
       61 cgggggtctc acatggtctc caagggggaa gaggacaaca tggcctctct gcctgccaca
      121 cacgagctgc acatcttcgg cagcatcaat ggcgtggact ttgatatggt gggccagggc
      181 acaggcaacc caaatgacgg ctacgaggag ctgaacctga agagcaccaa gggcgatctg
      241 cagttctccc cttggatcct ggtgccacac atcggctatg gctttcacca gtatctgccc
      301 taccctgacg gcatgtcccc cttccaggcc gccatggtgg atggctctgg ctaccaggtg
      361 cacaggacca tgcagtttga ggacggcgcc tccctgacag tgaattaccg gtatacctac
      421 gagggctctc acatcaaggg cgaggcccag gtgaagggca caggcttccc agccgatggc
      481 cccgtgatga caaactctct gaccgccgcc gactggtgcc ggagcaagaa gacctaccct
      541 aatgataaga caatcatctc cacctttaag tggtcttata ccacaggcaa cggcaagcgg
      601 tacagaagca cagccagaac cacatatacc tttgccaagc caatggccgc caactatctg
      661 aagaatcagc ccatgtacgt gttcaggaag acagagctga agcactccaa gaccgagctg
      721 aatttcaagg agtggcagaa ggcctttaca gacgtgatgg gcatggatga gctgtacaag
      781 gagtttggca ccgagaacct gtatctggag cacgacggct ctggcggcag catggtgttc
      841 acactggagg actttgtggg cgattggcgg cagaccgccg gctataatct ggatcaggtg
      901 ctggagcagg gcggcgtgag ctccctgttc cagaacctgg gcgtgagcgt gacccctatc
      961 cagcggatcg tgctgtccgg cgagaacggc ctgaagatcg acatccacgt gatcatccca
     1021 tacgagggcc tgtctggcga tcagatgggc cagatcgaga agatcttcaa ggtggtgtac
     1081 cccgtggacg atcaccactt caaagtgatc ctgcactatg gcaccctggt catcgacggc
     1141 gtgaccccca atatgatcga ttatttcggc cggccttacg agggcatcgc cgtgtttgac
     1201 ggcaagaaga tcaccgtgac aggcaccctg tggaacggca ataagatcat cgacgagaga
     1261 ctgatcaacc ccgatggctc cctgctgttc agggtgacta ttaacggagt gactggctgg
     1321 cggctgtgcg aaaggattct ggcttgactc gag
//
