LOCUS       sequence-181252-         169 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses ARiBo-fused RNA in vitro with T7 RNA polymerase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 169)
  AUTHORS   Di Tomasso G, Lampron P, Dagenais P, Omichinski JG, Legault P
  TITLE     The ARiBo tag: a reliable tool for affinity purification of RNAs 
            under native conditions.
  JOURNAL   Nucleic Acids Res. 2011 Feb;39(3):e18. doi: 10.1093/nar/gkq1084. 
            Epub 2010 Nov 11.
  PUBMED    21071425
REFERENCE   2  (bases 1 to 169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-02"; volume: "39(3):e18. doi"; pages: " 
            10.1093/nar/gkq1084. Epub 2010 Nov 11"
COMMENT     Sequence Label: pARiBo1
FEATURES             Location/Qualifiers
     source          1..169
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    36..50
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
ORIGIN
        1 taatacgact cactataggc gaagcgccga actgggccct gaagaagggc tcagttgacg
       61 aggtggggtt tatcgagatt tcggcggatg actcccggtt gttcatcaca accgcaaact
      121 tttacttaaa tcattaaggt gacttagtgg acaaaggtga aagtgtgat
//
