LOCUS       sequence-181546-        6261 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses the fat-7 gene in C. elegans intestine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Han S, Schroeder EA, Silva-Garcia CG, Hebestreit K, Mair WB, Brunet 
            A
  TITLE     Mono-unsaturated fatty acids link H3K4me3 modifiers to C. elegans 
            lifespan.
  JOURNAL   Nature. 2017 Apr 13;544(7649):185-190. doi: 10.1038/nature21686. 
            Epub 2017 Apr 5.
  PUBMED    28379943
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature21686"; journalName: "Nature"; date: "2017-04-13- 
            13"; volume: "544"; issue: "7649"; pages: "185-190"
COMMENT     Sequence Label: pSH1
FEATURES             Location/Qualifiers
     source          1..6261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    7..102
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3916..3935)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3917..3935)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3940..3956)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3940..3956)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4058..4077)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4069..4878
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4136..4155)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5025..5613
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5514..5533
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5767..5784
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5943..5970)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6062..6148)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     6197..6219
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6211..6228
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6212..6228
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agctccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata
       61 agcaatgctt ttttataatg ccaactttgt atagaaaagt tgaaactccg aactatgatg
      121 acgaaaaaat gttttcttga attttcattt tattgtgaaa ttgaaaactc tgcatagccc
      181 gcagtcactt gaaagcacat ttaagatgga atcaaatggt aagaaagctc gtgtaatcag
      241 gagattttat ccggggtatg ccgaggaata tatggtgcac tcgcattagc tggcattttt
      301 tgtaattatc ggatttctac ctttttctta atctagtttg ttatcattgt ccatcattta
      361 caaccaaaaa tttgcgtcat ttacaaccaa aaattgatct gacaattatc ataattcggt
      421 ttttattttc agaagaaacg ctccgatgtg atcctatagt gaatatactt ttccatcctc
      481 tctgaaataa acaattatga agaatgcatc aatttgcatt cgaaataact gccgtatttt
      541 gtctattggt atggctgcag taccattttt cttcgcgtct ttgcgcagcc ttcgggcgct
      601 accaataagg ctaagccggg gtaagttatt tcagaattct aatcaactaa tctaagtctc
      661 atacatcatt gtcaagtgac gcattttttt aatttctatg ttaactcgac tttaatattc
      721 ttttctggtt tcaccagaaa aatgctggac tgaaaaattg aatttcccgc gatttgggaa
      781 aaaccgcagt ttaagctttt ggcatgaata cagtgaacac aggaacaaaa aattgacttg
      841 ttattttgag tttatattaa atgttaattt tgcatctcat attttcacaa aacttgtttg
      901 cttgaaaaat gtgcaaataa caagtgaaca tacatcaagg tttatgcaga tcagcactga
      961 tgcaactgat agcaaaactg ataagggtca aaatttcagt ggccagcaca aacacataca
     1021 cattcgtagt tcgttttgtg tttcgtgtat ttagcattta tttttcaatt ccggcaattt
     1081 gccgatttgc cggaaatttt gatttttggc aaattgccag tttgccgttt gccgaacatc
     1141 aatttgccga aattttttag agggatcttt ataagacgga aacacttaaa attgtgcttt
     1201 tttggaattt ttttcaatgt ttcaatagaa ttagcttaca tttaaaatta gatgtaggaa
     1261 cgtttatagg acgcttacaa ttttgccgat taaaatcgaa gttctgaact tttcaaaaaa
     1321 tgtgcaaaat ttttttgaca aatttgacaa atcggccggt ttgtcgattg tccggaattt
     1381 ttcagtttcg gcgatttacc ggtttgccgg aaaacatcgt ttgccaccca cacacacata
     1441 aagagacgaa acaacacacc atcttcaaaa aatttgtgaa tcttcatttc cacgtggttt
     1501 gcagggtttc ccatccaccc cactctatct ctccaagttg aattttggca aaattgacca
     1561 cacaccgatc aaaaactatt gtgtgtgtgt gtatttgtgt cttcaataat tcctgtctgt
     1621 gtctcgcact atctattgac tacaagaacg tgatcgcagc tctacgaaac ctttacttta
     1681 ttttagaaat attttttcat gctattagca aaaaaaagtt tttttttgtc taagtacact
     1741 taaaattttc caaaattttt aagatttttc aaaatgtgcg atcaaagatc tgacaaaatg
     1801 ccaaaatttt gaagaggaaa tttagtcggc ttccaaaatt ataagtggca gaaattgagc
     1861 aattgtcact ttttgacagt aaatagaaaa ttttttgaaa aattttgaaa cgctttattt
     1921 tgttacttgg tcattttggc accataggag tagtttttaa cattttcccc actggcgcta
     1981 cttcatttta aacttttcca aagattttaa tttttaattt taaaaaattt catataattc
     2041 gtctgcgtct tacagtttca ggcttcaaaa atctattaca tatcttatct ttgaattcag
     2101 atgacggtaa aaactcgcgc gagcattgcc aagaagattg aaaaagatgg tttggactct
     2161 caatacctgt tcatggatcc aaatgaagtc ctgcaagtcc aagaggagag caaaaaagta
     2221 agctagtttt tgagagcgaa attcagatta gattctcaat atgaaatttt aaaaaaatta
     2281 aacaatacca tgagctaact gaattcagaa tctatttccc ttattccaga ttccatacaa
     2341 aatggaaata gtgtggcgta acgtggctct ctttgctgct ctgcacgtcg ccgcagccat
     2401 tggactttac gagcttgtct tccatgctaa atggcaaacg gccgtcttct gtaagttccc
     2461 ccaagcatta tttttgtgtc cggaaactag tattacttgt cacactaata gagaaataaa
     2521 ctactggatt ttgaaaacca aaaaactgta tatgaaaaga aacatttacg atgtctgtac
     2581 attttgaatg aaaagtttat tttttttaac tgattgcaat tgaacagaaa caaatatttc
     2641 gattagtgat aaacgagtga taaaaccagt tgtatattta attttcagca tttgctctct
     2701 atgtgttctc aggattcggt atcacagctg gagcccatcg tctctggtct cataaatcat
     2761 acaaggcaac cacaccaatg agaatctttt tgatgctctt gaacaacatt gctcttcaaa
     2821 acgacatcat tgaatgggct cgtgatcatc gttgccatca caagtggact gatacagatg
     2881 ctgatccaca taacaccacc cgtggattct tcttcactca catgggatgg cttcttgtgc
     2941 gtaagcatcc acaagttaag gagcatggag gcaaactcga cctatctgat ctatttagcg
     3001 atccagttct tgtattccag agaaagtaag ttaggttgaa ataaataaaa aatatgtcaa
     3061 ttttttattt caggcactat ttcccactgg tcattttgtt ttgtttcatt cttccaacaa
     3121 ttatccccgt ttatttctgg aaggagacag cattcattgc gttttacgta gctggaacat
     3181 tccgttattg tttcacactt cacgccactt ggtgcatcaa cagcgctgct cactagtaag
     3241 ttcactttat ttttatagat tcggggtcgt tttacaattt attacccagt gcaaaacaaa
     3301 aatttaaatt taaaatcttc aagtgtttag cgggaccaaa acaaagaaaa taggtaagtt
     3361 cgctgtcaaa atttctgtga tgacttggtc taaattaaag tttttaaaat acaaatgttt
     3421 tgtttttaag tttacggtaa aactgttttc ttttcatcgt tagaaaaata aatcaagctt
     3481 gaaattatta aattaaaatt ttcagttttg gttggaagcc atacgatact tctgtttccg
     3541 ccgttgagaa cgtctttacc acagttgtag ccgttggtga aggaggtcac aatttccacc
     3601 acacattccc acaagactac agagcatctg aatattcatt gatatacaat tggacccgtg
     3661 ttcttattga tactgcagct gttcttggtc ttgtctacga tagaaaaaca attgccgatg
     3721 agtttatcag ccggcaggtt gccaatcacg gaagtgaaga atcaagaaaa aaatcgatca
     3781 tgtaaattca ttatcattac gcgttggttg tccataaaag ttttaattga agcaaaaaat
     3841 aattaattta ttcctgaata aatttggtta attctaagaa ttcgagctct agagctgcag
     3901 ggcggccgcg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
     3961 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     4021 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     4081 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     4141 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
     4201 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     4261 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     4321 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
     4381 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     4441 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
     4501 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     4561 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     4621 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     4681 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     4741 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     4801 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     4861 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     4921 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg
     4981 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5041 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc tactagcggt ggtttgtttg
     5101 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5161 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5221 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5281 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5341 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5401 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5461 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5521 gcctggtatc tttatagtcc tgtcgggttt cgccacccct gacttgagcg tcgatttttg
     5581 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5641 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5701 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     5761 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     5821 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     5881 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
     5941 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
     6001 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
     6061 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     6121 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
     6181 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
     6241 cccgagttaa cgctaccatg g
//
