LOCUS       sequence-181809-       11513 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant 
            N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 
            under the control of TEF1/TEF2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11513)
  AUTHORS   Peng B, Nielsen LK, Kampranis SC, Vickers CE
  TITLE     Engineered protein degradation of farnesyl pyrophosphate synthase is
            an effective regulatory mechanism to increase monoterpene production
            in Saccharomyces cerevisiae.
  JOURNAL   Metab Eng. 2018 Feb 19. pii: S1096-7176(17)30214-8. doi: 
            10.1016/j.ymben.2018.02.005.
  PUBMED    29471044
REFERENCE   2  (bases 1 to 11513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2018 Feb 19. pii: S1096-7176(17)30214-8. doi: 
            10.1016/j.ymben.2018.02.005."
COMMENT     Sequence Label: pJT2
FEATURES             Location/Qualifiers
     source          1..11513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(771..818)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     1191..1209
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1247..1269)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1369..1388
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(1882..2976)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(2977..3384)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(3685..4140)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3822..3843)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4034..4053
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4266..4288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4280..4297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4281..4297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4307..4326
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4307..4325
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        6620..7033
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     complement(9129..9149)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9129..9147)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9168..9184)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9168..9184)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9181..9203)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9192..9208
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9216..9222,9223..9240,9241..9246))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9261..9282
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9399..9416)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9570..10158)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9650..9669)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10329..11120,11121..11189))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10952..10971
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11190..11294)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(11321..11513,1..1150)
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca
       61 aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa
      121 caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata
      181 actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc
      241 ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg
      301 tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat
      361 actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg
      421 gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt
      481 ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag taatactaga
      541 gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga
      601 tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa
      661 tgtttgtgga agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg
      721 gttttttgaa agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta
      781 tactttctag agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg
      841 cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat
      901 ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt
      961 aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg
     1021 atattatccc attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt
     1081 ctatatgctg ccactcctca attggattag tctcatcctt caatgctatc atttcctttg
     1141 atattggatc atactaagaa accattatta tcatgacatt aacctataaa aataggcgta
     1201 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     1261 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     1321 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     1381 agattgtact gagagtgcac catatcgact acgtcgttaa ggccgtttct gacagagtaa
     1441 aattcttgag ggaactttca ccattatggg aaatggttca agaaggtatt gacttaaact
     1501 ccatcaaatg gtcaggtcat tgagtgtttt ttatttgttg tatttttttt tttttagaga
     1561 aaatcctcca atatataaat taggaatcat agtttcatga ttttctgtta cacctaactt
     1621 tttgtgtggt gccctcctcc ttgtcaatat taatgttaaa gtgcaattct ttttccttat
     1681 cacgttgagc cattagtatc aatttgctta cctgtattcc tttacatcct cctttttctc
     1741 cttcttgata aatgtatgta gattgcgtat atagtttcgt ctaccctatg aacatattcc
     1801 attttgtaat ttcgtgtcgt ttctattatg aatttcattt ataaagttta tgtacaaata
     1861 tcataaaaaa agagaatctt tttaagcaag gattttctta acttcttcgg cgacagcatc
     1921 accgacttcg gtggtactgt tggaaccacc taaatcacca gttctgatac ctgcatccaa
     1981 aaccttttta actgcatctt caatggcctt accttcttca ggcaagttca atgacaattt
     2041 caacatcatt gcagcagaca agatagtggc gatagggttg accttattct ttggcaaatc
     2101 tggagcagaa ccgtggcatg gttcgtacaa accaaatgcg gtgttcttgt ctggcaaaga
     2161 ggccaaggac gcagatggca acaaacccaa ggaacctggg ataacggagg cttcatcgga
     2221 gatgatatca ccaaacatgt tgctggtgat tataatacca tttaggtggg ttgggttctt
     2281 aactaggatc atggcggcag aatcaatcaa ttgatgttga accttcaatg tagggaattc
     2341 gttcttgatg gtttcctcca cagtttttct ccataatctt gaagaggcca aaacattagc
     2401 tttatccaag gaccaaatag gcaatggtgg ctcatgttgt agggccatga aagcggccat
     2461 tcttgtgatt ctttgcactt ctggaacggt gtattgttca ctatcccaag cgacaccatc
     2521 accatcgtct tcctttctct taccaaagta aatacctccc actaattctc tgacaacaac
     2581 gaagtcagta cctttagcaa attgtggctt gattggagat aagtctaaaa gagagtcgga
     2641 tgcaaagtta catggtctta agttggcgta caattgaagt tctttacgga tttttagtaa
     2701 accttgttca ggtctaacac taccggtacc ccatttagga ccacccacag cacctaacaa
     2761 aacggcatca gccttcttgg aggcttccag cgcctcatct ggaagtggaa cacctgtagc
     2821 atcgatagca gcaccaccaa ttaaatgatt ttcgaaatcg aacttgacat tggaacgaac
     2881 atcagaaata gctttaagaa ccttaatggc ttcggctgtg atttcttgac caacgtggtc
     2941 acctggcaaa acgacgatct tcttaggggc agacattaga atggtatatc cttgaaatat
     3001 atatatatat attgctgaaa tgtaaaaggt aagaaaagtt agaaagtaag acgattgcta
     3061 accacctatt ggaaaaaaca ataggtcctt aaataatatt gtcaacttca agtattgtga
     3121 tgcaagcatt tagtcatgaa cgcttctcta ttctatatga aaagccggtt ccggcgctct
     3181 cacctttcct ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct
     3241 gaaattaaca aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg
     3301 ttctcgttat gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta
     3361 cgatacctga gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt
     3421 agaccgctcg gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa
     3481 tataacgttt ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat
     3541 gtggattttg atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt
     3601 agatatacta gaagttctcc tcgaccggtc gatatgcggt gtgaaatacc gcacagatgc
     3661 gtaaggagaa aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt
     3721 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     3781 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     3841 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     3901 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     3961 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     4021 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     4081 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     4141 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     4201 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     4261 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
     4321 atagggcgaa ttgggtaccg ggccctgcag actttgttat gtagagtttt tttagctacc
     4381 tatattccac cataacatca atcatgcggt tgctggtgta tttaccaata atgtttaatg
     4441 tatatatata tatatatatn nggggccgta tacttacata tagtagatgt caagcgtagg
     4501 cgcttcccct gccggctgtg agggcgccat aaccaaggta tctatagacc gccaatcagc
     4561 aaactacctc cgtacattca tgttgcaccc acacatttat acacccagac cgcgacaaat
     4621 tacccataag gttgtttgtg acggcgtcgt acaagagaac gtgggaactt tttaggctca
     4681 ccaaaaaaga aagaaaaaat acgagttgct gacagaagcc tcaagaaaaa aaaaattctt
     4741 cttcgactat gctggaggca gagatgatcg agccggtagt taactatata tagctaaatt
     4801 ggttccatca ccttcttttc tggtgtcgct ccttctagtg ctatttctgg cttttcctat
     4861 tttttttttt ccatttttct ttctctcttt ctaatatata aattctcttg cattttctat
     4921 ttttctctct atctattcta cttgtttatt cccttcaagg ttttttttta aggagtactt
     4981 gtttttagaa tatacggtca acgaactata attaactaaa catggcttca gaaaaagaaa
     5041 ttaggagaga gagattcttg aacgttttcc ctaaattagt agaggaattg aacgcatcgc
     5101 ttttggctta cggtatgcct aaggaagcat gtgactggta tgcccactca ttgaactaca
     5161 acactccagg cggtaagcta aatagaggtt tgtccgttgt ggacacgtat gctattctct
     5221 ccaacaagac cgttgaacaa ttggggcaag aagaatacga aaaggttgcc attctaggtt
     5281 ggtgcattga gttgttgcag gcttacttct tggtcgccga tgatatgatg gacaagtcca
     5341 ttaccagaag aggccaacca tgttggtaca aggttcctga agttggggaa attgccatct
     5401 gggacgcatt catgttagag gctgctatct acaagctttt gaaatctcac ttcagaaacg
     5461 aaaaatacta catagatatc accgaattgt tccatgaggt caccttccaa accgaattgg
     5521 gccaattgat ggacttaatc actgcacctg aagacaaagt cgacttgagt aagttctccc
     5581 taaagaagca ctccttcata gttactttca agactgctta ctattctttc tacttgcctg
     5641 tcgcattggc catgtacgtt gccggtatca cggatgaaaa ggatttgaaa caagccagag
     5701 atgtcttgat tccattgggt gaatacttcc aaattcaaga tgactactta gactgcttcg
     5761 gtaccccaga acagatcggt aagatcggta cagatatcca agataacaaa tgttcttggg
     5821 taatcaacaa ggcattagaa cttgcttccg cagaacaaag aaagacttta gacgaaaatt
     5881 acggtaagaa ggactcagtc gcagaagcca aatgcaaaaa gattttcaat gacttgaaaa
     5941 ttgaacagct ataccacgaa tatgaagagt ctattgccaa ggatttgaag gccaaaattt
     6001 ctcaggtcga tgagtctcgt ggcttcaaag ctgatgtctt aactgcgttc ttgaacaaag
     6061 tttacaagag aagcaaataa tagtgagaag ttttgttaga aaataaatca ttttttaatt
     6121 gagcattctt attcctattt tatttaaata gttttatgta ttgttagcta catacaacag
     6181 tttaaatcaa attttctttt tcccaagtcc aaaatggagg tttattttga tgacccgcat
     6241 gcgattatgt tttgaaagta taagactaca tacatgtaca tatatttaaa catgtaaacc
     6301 cgtccattat attgcttact ttcttctttt ttgccgtttt gacttggacc tctggtttgc
     6361 tatttcctta caatctttgc tacaatggat cccgggttga cagcctagac atcaatagtc
     6421 atacaacaga aagcgaccac ccaactttgg ctgataatag cgtataaaca atgcatactt
     6481 tgtacgttca aaatacaatg cagtagatat atttatgcat attacatata atacatatca
     6541 cataggaagc aacaggcgcg ttggactttt aattttcgag gaccgcgaat ccttacatca
     6601 cacccaatcc cccacaagtg atcccccaca caccatagct tcaaaatgtt tctactcctt
     6661 ttttactctt ccagattttc tcggactccg cgcatcgccg taccacttca aaacacccaa
     6721 gcacagcata ctaaatttcc cctctttctt cctctagggt gtcgttaatt acccgtacta
     6781 aaggtttgga aaagaaaaaa gagaccgcct cgtttctttt tcttcgtcga aaaaggcaat
     6841 aaaaattttt atcacgtttc tttttcttga aaattttttt ttttgatttt tttctctttc
     6901 gatgacctcc cattgatatt taagttaata aacggtcttc aatttctcaa gtttcagttt
     6961 catttttctt gttctattac aacttttttt acttcttgct cattagaaag aaagcatagc
     7021 aatctaatct aagttttaat tacaaaatgg ctaccgcagc aggtcctatc gcaactaaca
     7081 actccccaca aaactccaac gcttacagaa ctccaatcgc tccttccgta ccaattactc
     7141 ataaatggtc tatagctgaa gatttgacat gtatttccaa tcctagtaag cacaataacc
     7201 ctcaaactgg ttacagatca ttttctgacg aattatacgt taagtacgaa gaaaagttgg
     7261 aagatgttag aaaagcatta agagaagttg aagaaaaccc tttggaaggt ttagttatga
     7321 tagacgcttt gcaaagattg ggtatcgatt accatttcag aggtgaaatt ggtgcattct
     7381 tgcaaaagca acaaatcata tcttcaactc cagatggtta ccctgaacat ggtttgtacg
     7441 aagtttcaac attgtttaga ttcttaagac aagaaggtca caatgttacc gctgacgtct
     7501 ttaataactt caaggataag gaaggtagat tcagatcaga attgtcaaca gatattagag
     7561 gtttgatgtc cttatacgaa gcaagtcaat tgagaataga aggtgaagac atcttagatc
     7621 aagctgctga tttctccagt caattgttag gtagatggac aaaagatcct aatcatcacg
     7681 aagccagatt ggtttctaac actttaacac atccatacca caagtcattg gctaccttta
     7741 tgggtcaaaa attgtcctac atgaactgca agggtccaaa ctgggacggt gtcgataatt
     7801 tgcaagaatt agctaagatg gatttgacta tcgtacaaag tatccatcaa aaggaagtat
     7861 tccaagtttc tcaatggtgg aaggatacag gtttagccaa tgaattgaaa ttggctagaa
     7921 accaaccatt gaagtggtat atgtggccta tggccgcttt aaccgatcca agattcagtg
     7981 aagaaagagt tgaattaact aagcctattt cttttatata catcatagat gacatcttcg
     8041 acgtctatgg taccattgaa gaattgacct tgtttactga tgcagttaat agatgggaat
     8101 tgtctgccgt cgaacaatta ccagactaca tgaaagtatg tttcaaggct ttgtacgatg
     8161 ttaccaacga aatcgcatac aaaatctata aaaagcatgg tcaaaaccct attgattcct
     8221 tgcaaaagac ttgggctagt ttgtgcaatg catttttagt tgaagcaaag tggttcgcct
     8281 ctggtcactt gccaaatgca gaagaatact taaagaacgg tatcatctct tcaggtgttc
     8341 atgttgtctt ggcccacatg tttttcttgt taggtgacgg tattacacaa gaatcagttg
     8401 atttggtaga tgactatcca ggtatttcca caagtatcgc aaccattttg agattatctg
     8461 atgacttggg ttcagccaaa gatgaagacc aagatggtta tgatggttct tacatcgaat
     8521 gttacatgaa ggaacataag ggttccagtg tcgattcagc cagagaagaa gtaataagaa
     8581 tgatctccga agcatggaaa tgtttgaata aggaatgctt atcaccaaac cctttttctg
     8641 aatcattcag aataggttcc ttgaatatgg ctagaatgat ccctatgatg tactcttacg
     8701 atgacaacca taacttgcca attttagaag aacacatgaa ggcaatgatc tataacacat
     8761 ctttataata gtgagcggat tgagagcaaa tcgttaagtt caggtcaagt aaaaattgat
     8821 ttcgaaaact aatttctctt atacaatcct ttgattggac cgtcatcctt tcgaatataa
     8881 gattttgtta agaatatttt agacagagat ctactttata tttaatatct agatattaca
     8941 taatttcctc tctaataaaa tatcattaat aaaataaaaa tgaagcgatt tgattttgtg
     9001 ttgtcaactt agtttgccgc tatgcctctt gggtaatgct attattgaat cgaagggctt
     9061 tattatatta ccctttagct tattctgagg tttctgtggc gtccgcggtg gagctccagc
     9121 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
     9181 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     9241 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     9301 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     9361 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     9421 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     9481 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     9541 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     9601 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     9661 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     9721 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     9781 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9841 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9901 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9961 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
    10021 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    10081 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    10141 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    10201 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    10261 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    10321 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    10381 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    10441 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    10501 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    10561 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    10621 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    10681 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    10741 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10801 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10861 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10921 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10981 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    11041 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    11101 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    11161 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    11221 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    11281 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg aacgaagcat ctgtgcttca
    11341 ttttgtagaa caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg
    11401 catttttaca gaacagaaat gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc
    11461 ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaa
//
