LOCUS       sequence-181907-        9171 bp    DNA     linear   UNA 17-OCT-2025
DEFINITION  Destination vector with PGK -  Hygromycin selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Takashima Y, Guo G, Loos R, Nichols J, Ficz G, Krueger F, Oxley D, 
            Santos F, Clarke J, Mansfield W, Reik W, Bertone P, Smith A
  TITLE     Resetting Transcription Factor Control Circuitry toward Ground-State
            Pluripotency in Human.
  JOURNAL   Cell. 2014 Sep 11;158(6):1254-69. doi: 10.1016/j.cell.2014.08.029.
  PUBMED    25215486
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.08"; journalName: "Cell"; date: "2014-09-11- 
            11"; volume: "158"; issue: "6"; pages: "1254-69"
COMMENT     Sequence Label: pPB-PGK-destination
FEATURES             Location/Qualifiers
     source          1..9171
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     331..353
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     415..434
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    485..609
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(634..639,640..657,658..664)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             718..1377
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(784..803)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1719..2024
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1903..1922
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2065..2189)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2210..2226)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2234..2250
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2258..2264,2265..2282,2283..2288))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2303..2324
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2384..2401)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2390..2614
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    2691..2724
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   2895..2929
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2947..2966)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2948..2966)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2973..2990)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2973..2989)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2982..3004)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3131..3586
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3218..3237)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3428..3449
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3612..3716
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3717..3785,3786..4577)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3935..3954)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4748..5336
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5237..5256
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5490..5507
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5624..5645
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5660..5665,5666..5683,5684..5690)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5698..5714
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5722..5738
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   5796..5830
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     CDS             complement(6312..7337)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6312..7337)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7356..7855)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7404..7423)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     7797..7820
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    7878..7911
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        7927..8306
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8308..8583
                     /label=chicken beta-actin promoter
ORIGIN
        1 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
       61 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
      121 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
      181 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
      241 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
      301 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
      361 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
      421 tgctggttat tgtgctgtct catcattttg gcaaagaatt cctcgacggt atcgataagc
      481 tatcacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat
      541 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
      601 gtcactatgg cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
      661 aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg
      721 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat
      781 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt
      841 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac
      901 attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag
      961 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg
     1021 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg
     1081 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat
     1141 atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc
     1201 aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac
     1261 aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc
     1321 ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg
     1381 cgtggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc
     1441 ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga
     1501 agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga
     1561 tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc
     1621 cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat
     1681 gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct
     1741 ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc
     1801 ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc
     1861 gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata
     1921 tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa
     1981 atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta
     2041 tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat
     2101 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
     2161 tctcgttcag ctttcttgta caaagtggtg atagcttggc gtaatcatgg tcatagctgt
     2221 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     2281 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     2341 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat cagcctcgac tgtgccttct
     2401 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     2461 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     2521 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     2581 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     2641 ggctcgagat ccactagttc tagcctcgag gctagagcgg ccgcgtcgag ataacttcgt
     2701 ataatgtatg ctatacgaag ttatgttttg ttactttata gaagaaattt tgagtttttg
     2761 ttttttttta ataaataaat aaacataaat aaattgtttg ttgaatttat tattagtatg
     2821 taagtgtaaa tataataaaa cttaatatct attcaaatta ataaataaac ctcgagagca
     2881 atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt taaggtaccc
     2941 aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac
     3001 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     3061 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     3121 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3181 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     3241 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     3301 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     3361 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     3421 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     3481 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     3541 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     3601 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     3661 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     3721 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     3781 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     3841 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     3901 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     3961 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     4021 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     4081 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     4141 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     4201 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     4261 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     4321 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     4381 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     4441 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     4501 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     4561 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     4621 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     4681 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4741 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4801 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4861 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     4921 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4981 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5041 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5101 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5161 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     5221 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     5281 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     5341 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     5401 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     5461 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     5521 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     5581 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     5641 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     5701 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagctcgaaa
     5761 ttaaccctca ctatagggcg aattgggtac cttaacccta gaaagatagt ctgcgtaaaa
     5821 ttgacgcatg cattcttgaa atattgctct ctctttctaa atagcgcgaa tccgtcgctg
     5881 tgcatttagg acatctcagt cgccgcttgg agctcccgtg aggcgtgctt gtcaatgcgg
     5941 taagtgtcac tgattttgaa ctataacgac cgcgtgagtc aaaatgacgc atgattatct
     6001 tttacgtgac ttttaagatt taactcatac gataattata ttgttatttc atgttctact
     6061 tacgtgataa cttattatat atatattttc ttgttataga tatccaacta tgaaacatta
     6121 tcataaagca atagtaaaga gccttcagta aagagcaggc atttatctaa tcccacccca
     6181 cccccacccc cgtagctcca atccttccat tcaaaatgta ggtactctgt tctcaccctt
     6241 cttaacaaag tatgacagga aaaacttcca tgtggacatc tttattgttt aatagatcat
     6301 caatttctcg actattcctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg
     6361 gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta
     6421 cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct
     6481 gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc
     6541 atatacgccc ggagccgcgg cgatcctgca agctccggat gcctccgctc gaagtagcgc
     6601 gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat
     6661 tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc
     6721 gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc
     6781 tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc
     6841 atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat
     6901 gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga
     6961 tcggccgcag cgatcgcatc catggcctcc gcgaccggct gcagaacagc gggcagttcg
     7021 gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg
     7081 ctctcgctga attccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag
     7141 tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca
     7201 tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc
     7261 atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg
     7321 agttcaggct ttttcatggt ggcggctgga tcggtcgaaa ggcccggaga tgaggaagag
     7381 gagaacagcg cggcagacgt gcgcttttga agcgtgcaga atgccgggcc tccggaggac
     7441 cttcgggcgc ccgccccgcc cctgagcccg cccctgagcc cgcccccgga cccacccctt
     7501 cccagcctct gagcccagaa agcgaaggag caaagctgct attggccgct gccccaaagg
     7561 cctacccgct tccattgctc agcggtgctg tccatctgca cgagactagt gagacgtgct
     7621 acttccattt gtcacgtcct gcacgacgcg agctgcgggg cgggggggaa cttcctgact
     7681 aggggaggag tagaaggtgg cgcgaagggg ccaccaaaga acggagccgg ttggcgccta
     7741 ccggtggatg tggaatgtgt gcgaggccag aggccacttg tgtagcgcca agtgcccagc
     7801 ggggctgcta aagcgcatgc tccagactgc cttgggaaaa gcgcctcccc tacccggtag
     7861 aattcatcga tgatatcata acttcgtata atgtatgcta tacgaagtta tgcggccctc
     7921 gaggtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     7981 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     8041 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     8101 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     8161 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     8221 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     8281 acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc
     8341 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg
     8401 cagcgatggg ggcggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgggggg
     8461 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
     8521 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
     8581 gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
     8641 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
     8701 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
     8761 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
     8821 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
     8881 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
     8941 gggggcggtg ccccgcggtg cgggggggct gcgaggggaa caaaggctgc gtgcggggtg
     9001 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     9061 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     9121 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg c
//
