LOCUS       sequence-182122-       12105 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral 
            Replication: WDV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12105)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 12105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTRANS_233
FEATURES             Location/Qualifiers
     source          1..12105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1793..2344)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(2389..3065)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(2432..2452)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    3256..3277
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3292..3297,3298..3315,3316..3322)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3330..3346
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3335..3357
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3354..3370
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3354..3370
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    3828..3952
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3977..3982,3983..4000,4001..4007)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4061..4741
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             5061..5366
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5245..5264
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5407..5531)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    7335..7359
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8660..9289
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9718..10791
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10857..11051
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11287..11309)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11395..11535
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11450..11469
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11550..11567)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(11721..12105,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11801..11820)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agtcaaatct
     1801 cggtgacggg caggaccgga cggggcggta ccggcaggct gaagtccagc tgccagaaac
     1861 ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat
     1921 atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca
     1981 cgctcttgaa gccctgtgcc tccagggact tcagcagatg ggtgtagagc gtggagccca
     2041 gtcccgtccg ctggtggcgg gggctgacgt acacggtcga ctcggccgtc cagtcgtagg
     2101 cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc gacctcgccg tccacctcgg
     2161 cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt
     2221 cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc
     2281 agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc
     2341 tcatggtaga ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct
     2401 ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg
     2461 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt
     2521 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
     2581 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
     2641 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
     2701 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
     2761 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttcacatc
     2821 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
     2881 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt
     2941 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt
     3001 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag
     3061 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag
     3121 tgtcgtgctc caccatgttg gcaagctgct ctagccaata cgcaaaccgc ctctccccgc
     3181 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     3241 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     3301 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     3361 cagctatgac atgattacga attcgagtaa ggtagtgaac agaagtccgg caggtcctta
     3421 gcgaaaaaac ggggtgtgcc agaaaactct atcctctacc ctgcgtggag gtgtgaattc
     3481 tgcacactgc aaatgcaatg tgtccaatgc tttatatagg gcaggttttg gcgggagaac
     3541 agggccctag tgttcccacg gtagcgtagc gaatcgtgtg ggccctgttc ggtgtgcggt
     3601 cggggggcct ccacgcgggt tataatatta ccccgcgtgg tggcccccga cgcgcactcg
     3661 gcttttcgtg agtgcgcgga ggcttttgga ccacatcttt tctgatcact ttcgtggaag
     3721 atgttgattt atcacacttt tgacggggaa atctgtgcca tgccttagct tataaggaag
     3781 tgcgtggtag cccatctcga caagtttgta ccgatcaaaa gcaggtgaca agtttgtaca
     3841 aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc
     3901 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc
     3961 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag
     4021 ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     4081 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     4141 agtcgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     4201 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     4261 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
     4321 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     4381 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     4441 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
     4501 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     4561 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     4621 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
     4681 attacaacag tactgcgatg agtggcaggc ggggcgtaat ctagaggatc cggcttacta
     4741 aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact
     4801 gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga
     4861 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct
     4921 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga
     4981 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga
     5041 cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt
     5101 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga
     5161 tccccctggc cagtgcacgt ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg
     5221 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct
     5281 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
     5341 ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag
     5401 gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
     5461 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg
     5521 tacaaagtgg tcacctgcaa aaagtgtttg atcgccggcg gtacaaagtg gttaaaataa
     5581 tattttattt atctcatgtc attcgattac agaggctcgg ctacgagcaa agacaaacca
     5641 aatataacaa acaacaaccc ttacacaatg acatcggaaa acgaaataca acaccctgag
     5701 atattacatt tatagaaact gtacgccgtc cgcgctagga cagtcactgc gaagcagtga
     5761 cgtcttcgcc ggaggcgaac gagtagttga tgaacgtctc gccttcatac atgtagtgaa
     5821 caacagtgtt agagtacatg taatccgact gttcgggagt catatccttg agccaatctt
     5881 cgtctggatt aactaaaatg atgcaaggta ttccaccccg tatgaccttt cgcttaccat
     5941 attttggatt gaccgtgaag tcacgctgag ccccgacgaa gcacttccag ttgggtgtga
     6001 acttgaatgg aatgtcgtcg atgatattat acttggcgtt gacgtcatat gttgtgaaat
     6061 caactagact gttataataa ttgtgtgtcc ctagagacct tgcccaggaa gtctttcctg
     6121 ttctggttgg cccgcagatg tagatggact tatgcctccc cggtgactcc tggaataatc
     6181 gtccatccac tctaagtcag attgcgcttg atccgcagga gtggaagtac aaaggatata
     6241 ggattcgagg cttacggagt agagatgttc atttttccag ctttcaatgg tctcatggca
     6301 aatgagtgat tcggttggaa actcaggtgt gtaagtggca actgggtcag gaaatagatg
     6361 gcgtgccgtg tactcgaagt ctttgagacg gatagaccat tcaaacggaa aacgattgca
     6421 aaccatgctg aggaattcct cgcgagagga actagattca atgatctgtt tcatatccgc
     6481 atcacggtct ttacgacctg gagttgaaac agccacgaat gttccccact cagctgtgtt
     6541 tacatcggag tcaacctcct tcgtgatgta atcacgaact tggttgcagt ctttggcagc
     6601 ttgtatattt ggatggaata tggagaatgg agatgtatcc atacggaggt ttaaggcatt
     6661 gggattggtg atggaagcac gaagcttgtt ctgcacgaga acgtgcagat gtggtgatcc
     6721 atcttcgtgg agctctctaa cagcagcgat gtagaggggc tcatatttgt tcaagagagt
     6781 gcgaagtgaa tccaaggcgt actgtggctc aagggtacat tgaggatatg ttagaaagag
     6841 gtacttggaa tagacacgga acctgggtgc agatgaagag gccatggtag tgaacagaag
     6901 tccggcaggt ccttagcgaa aaaacggggt gtgccagaaa actctatcct ctaccctgcg
     6961 tggaggtgtg aattctgcac actgcaaatg caatgtgtcc aatgctttat atagggcagg
     7021 ttttggcggg agaacagggc cctagtgttc ccacggtagc gtagcgaatc gtgtgggccc
     7081 tgttcggtgt gcggtcgggg ggcctccacg cgggttataa tattaccccg cgtggtggcc
     7141 cccgacgcgc actcggcttt tcgtgagtgc gcggaggctt ttggaccaca tcttttctga
     7201 tcactttcgt ggaagatgtt gatttatcac acttttgacg gggaaatctg tgccatgcct
     7261 tagcttataa ggaagtgcgt ggtagcccat ctcggggccc tcgattcgac gttcctgttt
     7321 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
     7381 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
     7441 gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc
     7501 tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac
     7561 aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg
     7621 tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac
     7681 aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg
     7741 accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc
     7801 accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc
     7861 gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac
     7921 attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc
     7981 gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc
     8041 gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc
     8101 gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg
     8161 atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg
     8221 accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc
     8281 ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa
     8341 cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta
     8401 ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg
     8461 tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct
     8521 ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat
     8581 ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac
     8641 aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg
     8701 caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct
     8761 gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca
     8821 accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg
     8881 ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc
     8941 gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc
     9001 caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca
     9061 agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga
     9121 ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta
     9181 cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc
     9241 ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt
     9301 aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc
     9361 agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt
     9421 cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc
     9481 attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata
     9541 aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg
     9601 caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg
     9661 ctgggttgcc tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg
     9721 agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
     9781 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
     9841 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
     9901 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
     9961 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
    10021 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg
    10081 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
    10141 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
    10201 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
    10261 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
    10321 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
    10381 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc
    10441 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
    10501 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
    10561 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
    10621 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
    10681 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
    10741 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
    10801 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg
    10861 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
    10921 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
    10981 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
    11041 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
    11101 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
    11161 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
    11221 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg
    11281 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
    11341 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
    11401 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
    11461 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
    11521 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    11581 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    11641 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    11701 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    11761 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    11821 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    11881 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    11941 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    12001 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    12061 ccactggcag cagccactgg taacaggatt agcagagcga ggtat
//
