LOCUS       sequence-182243-        5610 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  AAV plasmid with smCBA promoter driving expression of iCre. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS2117
FEATURES             Location/Qualifiers
     source          1..5610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   1..130
                     /label=AAV2 ITR
     enhancer        276..579
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     1082..1101
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          1157..1289
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1272..1291
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    2370..2491
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2381..2400)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2435..2454
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2660..2789
                     /label=AAV2 ITR
     primer_bind     complement(2808..2825)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2808..2824)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2817..2839)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2966..3421
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3053..3072)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3263..3284
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3447..3551
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3552..3620,3621..4412)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3770..3789)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4583..5171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5072..5091
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5459..5480
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5495..5500,5501..5518,5519..5525)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5533..5549
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5538..5560
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5557..5573
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5557..5573
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcccta ggccggccgg taccctagtt attaatagta atcaattacg
      241 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      301 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      361 atagtaacgc caatagggac tttccattga cgtcaatggg tggactattt acggtaaact
      421 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      481 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      541 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc
      601 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
      661 taattatttt gtgcagcgat gggggcgggg gggggggagg ggcggggcgg ggcgaggcgg
      721 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
      781 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgacg
      841 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
      901 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
      961 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
     1021 ccgggagcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1081 ggcaacgtgc tggttattgt gctgtctcat cattttggca aaggcgcgcc gaagttggtc
     1141 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
     1201 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
     1261 tgacatccac tttgcctttc tctccacagg tgtccactcc caggcggccg ccaccatgtc
     1321 caacctgctg actgtgcacc aaaacctgcc tgccctccct gtggatgcca cctctgatga
     1381 agtcaggaag aacctgatgg acatgttcag ggacaggcag gccttctctg aacacacctg
     1441 gaagatgctc ctgtctgtgt gcagatcctg ggctgcctgg tgcaagctga acaacaggaa
     1501 atggttccct gctgaacctg aggatgtgag ggactacctc ctgtacctgc aagccagagg
     1561 cctggctgtg aagaccatcc aacagcacct gggccagctc aacatgctgc acaggagatc
     1621 tggcctgcct cgcccttctg actccaatgc tgtgtccctg gtgatgagga gaatcagaaa
     1681 ggagaatgtg gatgctgggg agagagccaa gcaggccctg gcctttgaac gcactgactt
     1741 tgaccaagtc agatccctga tggagaactc tgacagatgc caggacatca ggaacctggc
     1801 cttcctgggc attgcctaca acaccctgct gcgcattgcc gaaattgcca gaatcagagt
     1861 gaaggacatc tcccgcaccg atggtgggag aatgctgatc cacattggca ggaccaagac
     1921 cctggtgtcc acagctggtg tggagaaggc cctgtccctg ggggttacca agctggtgga
     1981 gagatggatc tctgtgtctg gtgtggctga tgaccccaac aactacctgt tctgccgggt
     2041 cagaaagaat ggtgtggctg ccccttctgc cacctcccaa ctgtccaccc gggccctgga
     2101 agggatcttt gaggccaccc accgcctgat ctatggtgcc aaggatgact ctgggcagag
     2161 atacctggcc tggtctggcc actctgccag agtgggtgct gccagggaca tggccagggc
     2221 tggtgtgtcc atccctgaaa tcatgcaggc tggtggctgg accaatgtga acatagtgat
     2281 gaactacatc agaaacctgg actctgagac tggggccatg gtgaggctgc tcgaggatgg
     2341 ggactgagcg gccgcgatcg cagacatgat aagatacatt gatgagtttg gacaaaccac
     2401 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     2461 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     2521 tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     2581 tagtcgaaat tcccgataag gatcttccta gagcatggct acgtagataa gtagcatggc
     2641 gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     2701 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     2761 gcggcctcag tgagcgagcg agcgcgcagc cttaattaac ctaattcact ggccgtcgtt
     2821 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     2881 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     2941 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3001 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3061 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3121 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     3181 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     3241 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     3301 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     3361 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     3421 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     3481 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     3541 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     3601 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     3661 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     3721 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     3781 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     3841 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     3901 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     3961 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4021 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     4081 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     4141 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     4201 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     4261 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     4321 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     4381 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     4441 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     4501 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4561 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4621 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4681 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     4741 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4801 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4861 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4921 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4981 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5041 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5101 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5161 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5221 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     5281 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     5341 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     5401 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     5461 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     5521 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     5581 acgccagatt taattaaggc cttaattagg
//
