LOCUS       sequence-18226-e         726 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Yu H, Peters JM, King RW, Page AM, Hieter P, Kirschner MW
  TITLE     Identification of a cullin homology region in a subunit of the 
            anaphase-promoting complex.
  JOURNAL   Science. 1998 Feb 20. 279(5354):1219-22.
  PUBMED    9469815
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1998 Feb 20. 279(5354):1219-22."
COMMENT     Sequence Label: pBlueBac human APC2
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             27..44
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             48..80
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(84..92,93..107)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 aacctataaa tatgccgcgg ggttctcatc atcatcatca tcatggtatg gctagcatga
       61 ctggtggaca gcaaatgggt cgggatctgt acgacgatga cgataaggat cgatggatcc
      121 gacctcgaga tctgcagctg gtaccatgga attcggcacg aggtcggtgg gtgcagatgg
      181 cggcggcagt tgtggtggcg gagggggaca gcgactcccg gcccggacag gagttgttag
      241 tggcctggaa caccgtgagc accggcctgg tgccgccggc tgcgctgggg ctggtgtctt
      301 cccggaccag cggtgcagtc ccgccaaagg aagaggagct ccgggcggcg gtggaggttc
      361 tgaggggcca cgggctacac tcggtcctgg aggagtggtt cgtggaggtg ctgcagaacg
      421 atctgcaggc caacatctcc cctgagttct ggaatgccat ctcccaatgc gagaactctg
      481 cggatgagcc ccagtgcctt ttgctactcc ttgacgcttt tggcctgctg gagagccgcc
      541 tggatcccta cctgcgtagc ctagagctgc tggagaaatg gactcgcctg ggcttgctga
      601 tgggcactgg tgctcagggg ctgcgagaag aagtccacac tatgttgcgc ggagtcttgt
      661 tctttagcac ccccagaacc ttccaagaga tgatccagcg tctgtatggg tgcttcttga
      721 gagtct
//
