LOCUS       sequence-182565-       10890 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Encodes Y2H Gal4 activation domain for Hsh155 K335T.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10890)
  AUTHORS   Carrocci TJ, Zoerner DM, Paulson JC, Hoskins AA
  TITLE     SF3b1 mutations associated with myelodysplastic syndromes alter the 
            fidelity of branchsite selection in yeast.
  JOURNAL   Nucleic Acids Res. 2017 Jan 6. pii: gkw1349. doi: 
            10.1093/nar/gkw1349.
  PUBMED    28062854
REFERENCE   2  (bases 1 to 10890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2017 Jan 6. pii: gkw1349. doi: 10.1093/nar/gkw1349."
COMMENT     Sequence Label: pGADT7 Hsh155 K335T
FEATURES             Location/Qualifiers
     source          1..10890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        771..1475
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1521..1541
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1557..1898
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     1904..1923
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1904..1922
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1929..1938
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1941..1967
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      5318..5505
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5373..5397)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(5622..6716)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(6717..7122)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(7164..7180)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7164..7180)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7177..7199)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7188..7204
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7212..7218,7219..7236,7237..7242))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7257..7278
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    7333..7366
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    7444..7477
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7546..7563)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7717..8305)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7797..7816)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8476..9267,9268..9336))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9099..9118
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9337..9441)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9509..9527
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9565..9587)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9687..9706
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      9723..10887
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc
       61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt
      121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca
      181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc
      241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg
      301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg
      361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa
      421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat
      481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg
      541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt
      601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac
      661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac
      721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt
      781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga
      841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat
      901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc
      961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata
     1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca
     1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat
     1141 gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca gcaccaacag
     1201 atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt tttccttcct
     1261 tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc cagttacttg
     1321 aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac ctgcaattat
     1381 taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc tttttctgca
     1441 caatatttca agctatacca agcatacaat caactccaag ctttgcaaag atggataaag
     1501 cggaattaat tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt accgccgcca
     1561 attttaatca aagtgggaat attgctgata gctcattgtc cttcactttc actaacagta
     1621 gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca caaccaattg
     1681 cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt aaaattgatg
     1741 atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg tataacgcgt
     1801 ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat aactatctat
     1861 tcgatgatga agatacccca ccaaacccaa aaaaagagat ctttaatacg actcactata
     1921 gggcgagcgc cgccatggag tacccatacg acgtaccaga ttacgctcat atggccatgg
     1981 aggccagtga attccacccg ggaatgagtc atccgattca atttgtaaac gctaataata
     2041 gcgataagag ccaccaatta ggcggtcaat attctattcc tcaggatttg agggaaaacc
     2101 tgcagaaaga agctgcacgt attggtgaga acgaaaaaga tgtgctacag gaaaaaatgg
     2161 aaactaggac agtgcaaaat cgagaggatt cataccacaa aagaaggttt gatatgaagt
     2221 ttgagccgga ttccgataca caaactgtta cgtcttcaga aaatacccaa gacgctgtcg
     2281 tgccaaggaa aagaaaatct cgttgggatg taaaagggta cgagccacct gatgaatctt
     2341 caacagcggt caaagaaaac tctgatagtg ctttggttaa tgtagaagga atccatgatt
     2401 tgatgttttt taaaccttct gaccataaat atttcgccga cgttatatcc aaaaaaccca
     2461 tagatgagtt aaataaggat gaaaagaaag aaagaaccct gtcgatgtta cttctgaaaa
     2521 taaaaaatgg caatacagcg agtaggagga cttctatgcg gattttaacc gataaagctg
     2581 ttacatttgg tccagaaatg atatttaacc gcttattgcc catattacta gacagaagtt
     2641 tggaagacca ggaaagacat ctaatgatca agaccataga tcgcgtcctt taccagctag
     2701 gagatttaac aaaaccttac gttcacaaaa tacttgtagt agctgcccca ttgctaatag
     2761 atgaagatcc aatggttcgt tcaacaggcc aggagataat caccaatctg tctacagtcg
     2821 ctggcctaaa aaccattcta acagtaatga gacctgatat tgagaatgag gatgagtatg
     2881 ttagaaatgt aacttcaaga gcggcggctg tggtggcaaa agcccttggt gttaatcaat
     2941 tgcttccttt catcaatgca gcatgccact cacggaagtc ttggaaagca aggcatactg
     3001 gcataactat tgtgcagcaa ataggtatac ttttgggaat aggtgtcttg aatcatctta
     3061 ctgggctgat gagttgtatc aaagactgcc taatggatga tcacgttccg gtgcgaattg
     3121 ttacagcaca tactttatca acactagcag aaaattctta tccatatggt atcgaagttt
     3181 tcaacgttgt actggagcca ttatggaagg gtataagaag tcatcgtggt aaagtgctat
     3241 cttctttttt aaaagctgtt ggatctatga tcccattgat ggacccagag tatgccggtt
     3301 attataccac agaagcaatg agaataatta gacgtgaatt cgactctccc gatgatgaaa
     3361 tgaaaaagac cattctttta gtattacaaa aatgtagcgc tgtagaatcc ataacgccaa
     3421 aatttctgag agaagaaatc gcgccagaat tctttcaaaa attttgggtc agacgagtgg
     3481 cattggatag accattaaac aaagttgtta catatactac tgttacatta gcaaagaaat
     3541 taggttgttc ttataccatt gataaattat taacaccatt gagagatgaa gctgagccgt
     3601 tcagaacaat ggctgtccat gctgttacta gaacagtaaa cttattaggg acagcagatt
     3661 tagatgagag attagaaacg aggcttattg atgcgctctt gatagcattt caagaacaaa
     3721 caaatagtga ttctataatc tttaaagggt ttggggcagt gacggtgtca cttgacatcc
     3781 gtatgaaacc gtttttagca cccattgtga gtacgatttt aaatcattta aagcataaaa
     3841 ctcccttggt tcgtcaacat gcagcagatc tatgtgccat actaatacca gtcatcaaga
     3901 actgtcacga attcgaaatg ttgaataaat tgaacattat attgtatgaa tccttaggcg
     3961 aagtatatcc agaggtatta ggttctataa tcaacgctat gtactgtatt actagcgtaa
     4021 tggatttgga taaactacaa cctcctatta atcaaatttt accaacctta actccaattt
     4081 taagaaacaa gcatagaaaa gtcgaagtaa acactatcaa atttgttgga cttattggta
     4141 aattagcacc tacctatgca cctcctaaag aatggatgag aatatgtttt gaactattag
     4201 aacttttgaa aagtacaaat aaagaaataa gaagatcagc aaatgccacg tttggattca
     4261 ttgctgaagc aattggacct cacgatgttc ttgttgcgtt attgaacaat ttaaaggttc
     4321 aagaacgtca gttacgtgta tgcacggctg ttgctatcgg tatcgttgct aaagtgtgtg
     4381 gtccttacaa cgtgctgcca gtaatcatga atgaatacac aacaccagag actaatgttc
     4441 aaaacggtgt tctcaaggcc atgtcattca tgtttgagta cattggtaac atgtctaagg
     4501 attacatata cttcataaca ccattattag aagatgcact caccgatagg gatttagttc
     4561 atcgtcagac agcgtcgaac gtaataaccc acttagcttt aaactgttca ggtacaggtc
     4621 acgaagatgc cttcattcat ttaatgaatc ttctaatacc caatattttt gaaacttcac
     4681 cacatgctat tatgcgtatt ttagaaggtt tggaggcact gagtcaagct cttggccctg
     4741 gactattcat gaactacata tgggcaggat tgttccatcc agcgaagaat gtaaggaaag
     4801 cattttggag ggtgtataat aacatgtacg taatgtacca ggacgccatg gtaccttttt
     4861 accccgttac accagacaac aatgaagaat atatagaaga actggattta gttctgtgag
     4921 gatccatcga gctcgagctg cagatgaatc gtagatactg aaaaaccccg caagttcact
     4981 tcaactgtgc atcgtgcacc atctcaattt ctttcattta tacatcgttt tgccttcttt
     5041 tatgtaacta tactcctcta agtttcaatc ttggccatgt aacctctgat ctatagaatt
     5101 ttttaaatga ctagaattaa tgcccatctt ttttttggac ctaaattctt catgaaaata
     5161 tattacgagg gcttattcag aagctttgga cttcttcgcc agaggtttgg tcaagtctcc
     5221 aatcaaggtt gtcggcttgt ctaccttgcc agaaatttac gaaaagatgg aaaagggtca
     5281 aatcgttggt agatacgttg ttgacacttc taaataagcg aatttcttat gatttatgat
     5341 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta
     5401 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc
     5461 tcaggtatag catgaggtcg ctcttattga ccacacctct accggccggt cgaaattccc
     5521 ctaccctatg aacatattcc attttgtaat ttcgtgtcgt ttctattatg aatttcattt
     5581 ataaagttta tgtacaaata tcataaaaaa agagaatctt tttaagcaag gattttctta
     5641 acttcttcgg cgacagcatc accgacttcg gtggtactgt tggaaccacc taaatcacca
     5701 gttctgatac ctgcatccaa aaccttttta actgcatctt caatggcctt accttcttca
     5761 ggcaagttca atgacaattt caacatcatt gcagcagaca agatagtggc gatagggttg
     5821 accttattct ttggcaaatc tggagcagaa ccgtggcatg gttcgtacaa accaaatgcg
     5881 gtgttcttgt ctggcaaaga ggccaaggac gcagatggca acaaacccaa ggaacctggg
     5941 ataacggagg cttcatcgga gatgatatca ccaaacatgt tgctggtgat tataatacca
     6001 tttaggtggg ttgggttctt aactaggatc atggcggcag aatcaatcaa ttgatgttga
     6061 accttcaatg taggaaattc gttcttgatg gtttcctcca cagtttttct ccataatctt
     6121 gaagaggcca aaacattagc tttatccaag gaccaaatag gcaatggtgg ctcatgttgt
     6181 agggccatga aagcggccat tcttgtgatt ctttgcactt ctggaacggt gtattgttca
     6241 ctatcccaag cgacaccatc accatcgtct tcctttctct taccaaagta aatacctccc
     6301 actaattctc tgacaacaac gaagtcagta cctttagcaa attgtggctt gattggagat
     6361 aagtctaaaa gagagtcgga tgcaaagtta catggtctta agttggcgta caattgaagt
     6421 tctttacgga tttttagtaa accttgttca ggtctaacac tacctgtacc ccatttagga
     6481 ccacccacag cacctaacaa aacggcatca gccttcttgg aggcttccag cgcctcatct
     6541 ggaagtggga cacctgtagc ttcgatagca gcaccaccaa ttaaatgatt ttcgaaatcg
     6601 aacttgacat tggaacgaac atcagaaata gctttaagaa ccttaatggc ttcggctgtg
     6661 atttcttgac caacgtggtc acctggcaaa acgacgatct tcttaggggc agacattaga
     6721 atggtatatc cttgaaatat atatatatat tgctgaaatg taaaaggtaa gaaaagttag
     6781 aaagtaagac gattgctaac cacctattgg aaaaaacaat aggtccttaa ataatattgt
     6841 caacttcaag tattgtgatg caagcattta gtcatgaacg cttctctatt ctatatgaaa
     6901 agccggttcc ggcgctctca cctttccttt ttctcccaat ttttcagttg aaaaaggtat
     6961 atgcgtcagg cgacctctga aattaacaaa aaatttccag tcatcgaatt tgattctgtg
     7021 cgatagcgcc cctgtgtgtt ctcgttatgt tgaggaaaaa aataatggtt gctaagagat
     7081 tcgaactctt gcatcttacg atacctgagt attcccacag ttgggggatc tcgactctag
     7141 ctagaggatc aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7201 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7261 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7321 tcgtgccagc tgataacttc gtataatgta tgctatacga agttattagg tctgaagagg
     7381 agtttacgtc cagccaagct agcttggctg caggtcgagc ggccgcgatc cggaaccctt
     7441 aatataactt cgtataatgt atgctatacg aagttatcag ctgcattaat gaatcggcca
     7501 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7561 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7621 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7681 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7741 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7801 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7861 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7921 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7981 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8041 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8101 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8161 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8221 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8281 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8341 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8401 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8461 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8521 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8581 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8641 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8701 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8761 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8821 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8881 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8941 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9001 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9061 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9121 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9181 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9241 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9301 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9361 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9421 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     9481 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     9541 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     9601 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     9661 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     9721 taacgcattt aagcataaac acgcactatg ccgttcttct catgtatata tatatacagg
     9781 caacacgcag atataggtgc gacgtgaaca gtgagctgta tgtgcgcagc tcgcgttgca
     9841 ttttcggaag cgctcgtttt cggaaacgct ttgaagttcc tattccgaag ttcctattct
     9901 ctagctagaa agtataggaa cttcagagcg cttttgaaaa ccaaaagcgc tctgaagacg
     9961 cactttcaaa aaaccaaaaa cgcaccggac tgtaacgagc tactaaaata ttgcgaatac
    10021 cgcttccaca aacattgctc aaaagtatct ctttgctata tatctctgtg ctatatccct
    10081 atataaccta cccatccacc tttcgctcct tgaacttgca tctaaactcg acctctacat
    10141 tttttatgtt tatctctagt attactcttt agacaaaaaa attgtagtaa gaactattca
    10201 tagagtgaat cgaaaacaat acgaaaatgt aaacatttcc tatacgtagt atatagagac
    10261 aaaatagaag aaaccgttca taattttctg accaatgaag aatcatcaac gctatcactt
    10321 tctgttcaca aagtatgcgc aatccacatc ggtatagaat ataatcgggg atgcctttat
    10381 cttgaaaaaa tgcacccgca gcttcgctag taatcagtaa acgcgggaag tggagtcagg
    10441 ctttttttat ggaagagaaa atagacacca aagtagcctt cttctaacct taacggacct
    10501 acagtgcaaa aagttatcaa gagactgcat tatagagcgc acaaaggaga aaaaaagtaa
    10561 tctaagatgc tttgttagaa aaatagcgct ctcgggatgc atttttgtag aacaaaaaag
    10621 aagtatagat tctttgttgg taaaatagcg ctctcgcgtt gcatttctgt tctgtaaaaa
    10681 tgcagctcag attctttgtt tgaaaaatta gcgctctcgc gttgcatttt tgttttacaa
    10741 aaatgaagca cagattcttc gttggtaaaa tagcgctttc gcgttgcatt tctgttctgt
    10801 aaaaatgcag ctcagattct ttgtttgaaa aattagcgct ctcgcgttgc atttttgttc
    10861 tacaaaatga agcacagatg cttcgttgct
//
