LOCUS       sequence-182626-        9100 bp    DNA     circular SYN 15-APR-2025
DEFINITION  a derivative of pDIRECT_37J with translation initiation sequence 
            optimized for dicots.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9100)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 9100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTC102
FEATURES             Location/Qualifiers
     source          1..9100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(141..160)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(820..1635)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(820..1635)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1543..1562
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1627..1646
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1926..2361
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2508..2532
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(2555..2807)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(3922..4105)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(3958..4137)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4716..5061
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5004..5024
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             5095..5160
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5167..5187
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(5626..5931)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(5728..5747)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             7005..7061
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7065..7130
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7137..7157
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(7485..7892)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     7742..7764
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7756..7773
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7757..7773
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7783..7802
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7783..7801
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7849..7869)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7849..7867)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7888..7904)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7888..7904)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7901..7923)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7912..7928
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7936..7942,7943..7960,7961..7966))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7981..8002
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             8188..8775
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRDKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     terminator      8789..9037
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    9044..9068
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
       61 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      121 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      181 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      241 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      301 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      361 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      421 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      481 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      541 cttcggaaga agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      601 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      661 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      721 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      781 atcaatctaa agtatatatg tgtaacattg gtctagtgat tagaaaaact catcgagcat
      841 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      901 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      961 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     1021 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     1081 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     1141 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gccgaaatac
     1201 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     1261 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     1321 tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     1381 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     1441 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     1501 cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     1561 cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg
     1621 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     1681 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     1741 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     1801 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     1861 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc
     1921 gatccccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata
     1981 gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt
     2041 ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa
     2101 tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga
     2161 gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt
     2221 tacagcaagt agtatgttca attagctttt caattatgaa tatatatatc aattattggt
     2281 cgcccttggc ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag
     2341 cggttgccca ccgtcgagcg cctttgccca caacccggcg gccggccgca acagatcgtt
     2401 ttataaattt ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct
     2461 ggatttccga tccccggaat tagatccgtt taaactacgt aagatcctgg caggatatat
     2521 tgtggtgtaa acgtgtacgc gttgcatgcc ggtcgatcta gtaacataga tgacaccgcg
     2581 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
     2641 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
     2701 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
     2761 acaggattca atcttaagaa actttattgc caaatgtttg aacgatcggg gaaattcggg
     2821 ggatcagctt gtgatcatgt tacatcacct ccaagatccc ggacgagtgc tggggcgtcg
     2881 gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg
     2941 ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc
     3001 ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag
     3061 accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg gatgcctccg
     3121 ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt
     3181 ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat
     3241 gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac
     3301 ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact
     3361 gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat
     3421 gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct
     3481 cgtctggcta agatcggccg cagcgatcgc atccatggcc tccgcgaccg gctgcagaac
     3541 agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat
     3601 gcaataggtc aggctctcgc tgaattcccc aatgtcaagc acttccggaa tcgggagcgc
     3661 ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt
     3721 tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc
     3781 ctccgagagc tgcatcaggt cggacacgct gtcgaacttt tcgatcagaa acttctcgac
     3841 agacgtcgcg gtgagttcag gctttttcat atctcattgc cccccgggat cttgcaggtc
     3901 gagatcctct agcggatccg gtgcagatta tttggattga gagtgaatat gagactctaa
     3961 ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag aaatatttgc
     4021 tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg acgtatgtgc
     4081 ttagctcatt aaactccaga aacccgcggc tgagtggctc cttcaacgtt gcggttctgt
     4141 cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa cgtgactccc
     4201 ttaattctcc gctcatgatc ggcgcgccag atttgccttt tcaatttcag aaagaatgct
     4261 aacccacaga tggttagaga ggcttacgca gcaggtatca tcaagacgat ctacccgagc
     4321 aataatctcc aggaaatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc
     4381 aggactaact gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt
     4441 ccagtatgga cgattcaagg cttgcttcac aaaccaaggc aagtaataga gattggagtc
     4501 tctaaaaagg tagttcccac tgaatcaaag gccatggagt caaagattca aatagaggac
     4561 ctaacagaac tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat
     4621 gacaagaaga aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat
     4681 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
     4741 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
     4801 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
     4861 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     4921 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
     4981 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
     5041 tcatttcatt tggagagaac acgggggact cctgcaggaa aaatggcttc ctccgattat
     5101 aaggatcacg atggcgacta caaggaccac gatattgact acaaagacga tgacgataaa
     5161 atggctccta agaaaaagcg caaagtcggt atccatggcg ttccctctag aatggtggat
     5221 ctacgcacgc tcggctacag tcagcagcag caagagaaga tcaaaccgaa ggtgcgttcg
     5281 acagtggcgc agcaccacga ggcactggtg ggccatgggt ttacacacgc gcacatcgtt
     5341 gcgctcagcc aacacccggc agcgttaggg accgtcgctg tcacgtatca gcacataatc
     5401 acggcgttgc cagaggcgac acacgaagac atcgttggcg tcggcaaaca gtggtccggc
     5461 gcacgcgccc tggaggcctt gctcacggat gcgggggagt tgagaggtcc gccgttacag
     5521 ttggacacag gccaacttgt gaagattgca aaacgtggcg gcgtgaccgc aatggaggca
     5581 gtgcatgcca gcaggaatgc gttaacaggt gcccccctgg agacgttata ttccccagaa
     5641 catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc
     5701 cccgataacg gaaaccggca cactggccat atcggtggtc atcatgcgcc agctttcatc
     5761 cccgatatgc accaccgggt aaagttcacg ggagacttta tctgacagca gacgtgcact
     5821 ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata atatcactct gtacatccac
     5881 aaacagacga taacggctct ctcttttata ggtgtaaacc ttaaactgca tttcaccagc
     5941 ccctgttctc gtcagcaaaa gagccgttca tttcaataaa ccgggcgacc tcagccatcc
     6001 cttcctgatt ttccgctttc cagcgttcgg cacgcagacg acgggcttca ttctgcatgg
     6061 ttgtgcttac cagaccggag atattgacat catatatgcc ttgagcaact gatagctgtc
     6121 gctgtcaact gtcactgtaa tacgctgctt catagcatac ctccttttga catacttcgg
     6181 gtatacatat cagtatatat tcttataccg caaaaatcag cgcgcaaata cgcatactgt
     6241 tatctggctt cgtctccaac gaccacctcg tcgccttggc ctgcctcggc ggacgtcctg
     6301 ccatggatgc agtgaaaaag ggattgccgc acgcgccgga attgatcaga agagtcaatc
     6361 gccgtattgg cgaacgcacg tcccatcgcg ttgccggatc ccagctggtg aagtccgagc
     6421 tggaagaaaa aaagagcgag ctgcgccaca agctcaagta cgtgccccac gagtacatcg
     6481 agctgatcga gatcgcccgc aacagcaccc aagaccgcat cctggagatg aaagtgatgg
     6541 agttcttcat gaaggtgtac ggctaccgcg gcaagcacct gggcggctcc cgcaagcccg
     6601 atggcgccat ctacaccgtg ggctccccca tcgactatgg cgtcattgtc gacaccaagg
     6661 cctactccgg cggctacaac ttacccatcg gtcaggccga cgagatgcaa cgctacgtga
     6721 aggagaacca gacccgcaat aagcacatta atcccaacga gtggtggaag gtgtacccct
     6781 cctccgtgac cgagttcaaa ttcctgttcg tgtccggcca cttcaagggc aattataagg
     6841 cccaactgac ccgcctgaac cgcaagacca actgcaacgg cgccgtgctg tccgtggagg
     6901 aactgctgat cggcggcgag atgatcaagg ctggtaccct gaccctggaa gaggtgcgcc
     6961 gcaagttcaa caatggtgaa atcaatttca ggtccggaag cggcgccacg aacttctctc
     7021 tgttaaagca agcaggtgac gtggaagaaa acccagggcc catggactac aaagaccatg
     7081 acggtgatta taaagatcat gacatcgatt acaaggatga cgatgacaag atggccccca
     7141 agaagaagag gaaggtgggc attcacgggg tgccggctag catggtggat ctacgcacgc
     7201 tcggctacag tcagcagcag caagagaaga tcaaaccgaa ggtgcgttcg acagtggcgc
     7261 agcaccacga ggcactggtg ggccatgggt ttacacacgc gcacatcgtt gcgctcagcc
     7321 aacacccggc agcgttaggg accgtcgctg tcacgtatca gcacataatc acggcgttgc
     7381 cagaggcgac acacgaagac atcgttggcg tcggcaaaca gtggtccggc gcacgcgccc
     7441 tggaggcctt gctcacggat gcgggggagt tgagaggtcc gccgttacag ttggacacag
     7501 gccaacttgt gaagattgca aaacgtggcg gcgtgaccgc aatggaggca gtgcatgcat
     7561 cgcgcaatgc actgacgggt gcccccctgg agaccggcgc cgctacaggg cgcgtcccat
     7621 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     7681 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     7741 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     7801 ggcgaattgg gtaccgggcc gccccttgag gtcctccagc ttttgttccc tttagtgagg
     7861 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     7921 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7981 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc accggtggtc
     8041 tccaacgatc atctggtggc actcgcgtgc ctcggcggac gtcctgccat ggatgcagtg
     8101 aaaaagggat tgccgcacgc gccggaattg atcagaagag tcaatcgccg tattggcgaa
     8161 cgcacgtccc atcgcgttgc cagatctcaa ctagtcaaaa gtgaactgga ggagaagaaa
     8221 tctgaacttc gtcataaatt gaaatatgtg cctcatgaat atattgaatt aattgaaatt
     8281 gccagaaatt ccactcagga tagaattctt gaaatgaagg taatggaatt ttttatgaaa
     8341 gtttatggat atagaggtaa acatttgggt ggatcaagga aaccggacgg agcaatttat
     8401 actgtcggat ctcctattga ttacggtgtg atcgtggata ctaaagctta tagcggaggt
     8461 tataatctgc caattggcca agcagatgaa atggagcgat atgtcgaaga aaatcaaaca
     8521 cgagacaaac atctcaaccc taatgaatgg tggaaagtct atccatcttc tgtaacggaa
     8581 tttaagtttt tatttgtgag tggtcacttt aaaggaaact acaaagctca gcttacacga
     8641 ttaaatcata tcactaattg taatggagct gttcttagtg tagaagagct tttaattggt
     8701 ggagaaatga ttaaagccgg cacattaacc ttagaggaag tgaggcggaa atttaataac
     8761 ggcgagataa acttttaata gctcgagata tgaagatgaa gatgaaatat ttggtgtgtc
     8821 aaataaaaag cttgtgtgct taagtttgtg tttttttctt ggcttgttgt gttatgaatt
     8881 tgtggctttt tctaatatta aatgaatgta agatcacatt ataatgaata aacaaatgtt
     8941 tctataatcc attgtgaatg ttttgttgga tctcttctgc agcatataac tactgtatgt
     9001 gctatggtat ggactatgga atatgattaa agataaggag ctctgacagg atatattggc
     9061 gggtaaacta agtcgctgta tgtgtttgtt tgatttaaat
//
