LOCUS       sequence-18272-e         872 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Ueki K, Kondo T, Kahn CR
  TITLE     Suppressor of cytokine signaling 1 (SOCS-1) and SOCS-3 cause insulin
            resistance through inhibition of tyrosine phosphorylation of insulin
            receptor substrate proteins by discrete mechanisms.
  JOURNAL   Mol Cell Biol. 2004 Jun . 24(12):5434-46.
  PUBMED    15169905
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2004 Jun . 24(12):5434-46."
COMMENT     Sequence Label: pCMV SOCS-3
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             99..122
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(836..852)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(837..853)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gaccgtcaga tccgctagcg attacgccaa gctcgaaatt aaccctcact aaagggaaca
       61 aaagctggag ctccaccgcg gtggcggccg ccaccatgga ttacaaggat gacgacgata
      121 agagcccggg cggatccatg gtcacccaca gcaagtttcc cgccgccggg atgagccgcc
      181 ccctggacac cagcctgcgc ctcaagacct tcagctccaa aagcgagtac cagctggtgg
      241 tgaacgccgt gcgcaagctg caggagagcg gattctactg gagcgccgtg accggcggcg
      301 aggcgaacct gctgctcagc gccgagcccg cgggcacctt tcttatccgc gacagctcgg
      361 accagcgcca cttcttcacg ttgagcgtca agacccagtc ggggaccaag aacctacgca
      421 tccagtgtga ggggggcagc ttttcgctgc agagtgaccc ccgaagcacg cagccagttc
      481 cccgcttcga ctgtgtactc aagctggtgc accactacat gccgcctcca gggaccccct
      541 ccttttcttt gccacccacg gaaccctcgt ccgaagttcc ggagcagcca cctgcccagg
      601 cactccccgg gagtaccccc aagagagctt actacatcta ttctgggggc gagaagattc
      661 cgctggtact gagccgacct ctctcctcca acgtggccac cctccagcat ctttgtcgga
      721 agactgtcaa cggccacctg gactcctatg agaaagtgac ccagctgcct ggacccattc
      781 gggagttcct ggatcagtat gatgctccac tttaggaatt cgatatcaag cttatcgata
      841 ccgtcgacct cgaggggggg cccggtacct ta
//
