LOCUS       sequence-182879-       10188 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Encodes Prp2 Q548N for expression in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10188)
  AUTHORS   Carrocci TJ, Zoerner DM, Paulson JC, Hoskins AA
  TITLE     SF3b1 mutations associated with myelodysplastic syndromes alter the 
            fidelity of branchsite selection in yeast.
  JOURNAL   Nucleic Acids Res. 2017 Jan 6. pii: gkw1349. doi: 
            10.1093/nar/gkw1349.
  PUBMED    28062854
REFERENCE   2  (bases 1 to 10188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2017 Jan 6. pii: gkw1349. doi: 10.1093/nar/gkw1349."
COMMENT     Sequence Label: pRS415 Prp2 Q548N
FEATURES             Location/Qualifiers
     source          1..10188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(664..1758)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(1759..2166)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(2467..2922)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2604..2625)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2816..2835
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3048..3070
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3062..3079
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3063..3079
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3089..3108
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3089..3107
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3132..3148
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3133..3149
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7361..7377)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7361..7377)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7414..7434)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7414..7432)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7453..7469)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7453..7469)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7466..7488)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7477..7493
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7501..7507,7508..7525,7526..7531))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7546..7567
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7684..7701)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7855..8443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7935..7954)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8614..9405,9406..9474))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9237..9256
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9475..9579)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    9616..10119
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     10161..10179
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatcga ctacgtcgtt aaggccgttt ctgacagagt aaaattcttg agggaacttt
      241 caccattatg ggaaatggtt caagaaggta ttgacttaaa ctccatcaaa tggtcaggtc
      301 attgagtgtt ttttatttgt tgtatttttt tttttttaga gaaaatcctc caatatataa
      361 attaggaatc atagtttcat gattttctgt tacacctaac tttttgtgtg gtgccctcct
      421 ccttgtcaat attaatgtta aagtgcaatt ctttttcctt atcacgttga gccattagta
      481 tcaatttgct tacctgtatt cctttacatc ctcctttttc tccttcttga taaatgtatg
      541 tagattgcgt atatagtttc gtctacccta tgaacatatt ccattttgta atttcgtgtc
      601 gtttctatta tgaatttcat ttataaagtt tatgtacaaa tatcataaaa aaagagaatc
      661 tttttaagca aggattttct taacttcttc ggcgacagca tcaccgactt cggtggtact
      721 gttggaacca cctaaatcac cagttctgat acctgcatcc aaaacctttt taactgcatc
      781 ttcaatggcc ttaccttctt caggcaagtt caatgacaat ttcaacatca ttgcagcaga
      841 caagatagtg gcgatagggt tgaccttatt ctttggcaaa tctggagcag aaccgtggca
      901 tggttcgtac aaaccaaatg cggtgttctt gtctggcaaa gaggccaagg acgcagatgg
      961 caacaaaccc aaggaacctg ggataacgga ggcttcatcg gagatgatat caccaaacat
     1021 gttgctggtg attataatac catttaggtg ggttgggttc ttaactagga tcatggcggc
     1081 agaatcaatc aattgatgtt gaaccttcaa tgtagggaat tcgttcttga tggtttcctc
     1141 cacagttttt ctccataatc ttgaagaggc caaaacatta gctttatcca aggaccaaat
     1201 aggcaatggt ggctcatgtt gtagggccat gaaagcggcc attcttgtga ttctttgcac
     1261 ttctggaacg gtgtattgtt cactatccca agcgacacca tcaccatcgt cttcctttct
     1321 cttaccaaag taaatacctc ccactaattc tctgacaaca acgaagtcag tacctttagc
     1381 aaattgtggc ttgattggag ataagtctaa aagagagtcg gatgcaaagt tacatggtct
     1441 taagttggcg tacaattgaa gttctttacg gatttttagt aaaccttgtt caggtctaac
     1501 actaccggta ccccatttag gaccacccac agcacctaac aaaacggcat cagccttctt
     1561 ggaggcttcc agcgcctcat ctggaagtgg aacacctgta gcatcgatag cagcaccacc
     1621 aattaaatga ttttcgaaat cgaacttgac attggaacga acatcagaaa tagctttaag
     1681 aaccttaatg gcttcggctg tgatttcttg accaacgtgg tcacctggca aaacgacgat
     1741 cttcttaggg gcagacatta gaatggtata tccttgaaat atatatatat atattgctga
     1801 aatgtaaaag gtaagaaaag ttagaaagta agacgattgc taaccaccta ttggaaaaaa
     1861 caataggtcc ttaaataata ttgtcaactt caagtattgt gatgcaagca tttagtcatg
     1921 aacgcttctc tattctatat gaaaagccgg ttccggcgct ctcacctttc ctttttctcc
     1981 caatttttca gttgaaaaag gtatatgcgt caggcgacct ctgaaattaa caaaaaattt
     2041 ccagtcatcg aatttgattc tgtgcgatag cgcccctgtg tgttctcgtt atgttgagga
     2101 aaaaaataat ggttgctaag agattcgaac tcttgcatct tacgatacct gagtattccc
     2161 acagttaact gcggtcaaga tatttcttga atcaggcgcc ttagaccgct cggccaaaca
     2221 accaattact tgttgagaaa tagagtataa ttatcctata aatataacgt ttttgaacac
     2281 acatgaacaa ggaagtacag gacaattgat tttgaagaga atgtggattt tgatgtaatt
     2341 gttgggattc catttttaat aaggcaataa tattaggtat gtagatatac tagaagttct
     2401 cctcgaccgg tcgatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     2461 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     2521 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     2581 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     2641 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     2701 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     2761 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     2821 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     2881 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
     2941 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     3001 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     3061 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
     3121 cgggcccccc ctcgaggtcg acggtatcga taagcttgat atcgaattcc tgcagcccgg
     3181 gggatccgcg atggaatgat agcatctgca ttcttcattg ttggtttcac aaactttacc
     3241 gcatttggtt tcacaaattt ctcccattgt tgaatacaac catccaaatc tctccctctg
     3301 gaaactatat ctctcgacaa tcttcttgct aagcagacat ccaaatcagc gtcaacataa
     3361 attttcaagt ccatcaaatc cagcaatcgg cgatcgtaaa gggcgtagat cccttcgata
     3421 actaccacac tggccccgta tatgactata tttttatcag gaactctatt gtggtggacg
     3481 aagctataaa ctgggatatt tgtccttttg ccctccttta agttcaaaat gcacttatat
     3541 gccaaatcta agttgatggc atttggctcg tcgaaatcgt attcattttt aaaggctctg
     3601 gctctgtcct ctgggcctaa tggattgtaa aagttatcca aagatattaa tactgtccag
     3661 ggaacattaa ttgacgacac aatcttagca gcaacgcttg tcttgcctga acctgaagca
     3721 ccacctatac ctattatata tggagttgtc catggtggga tataccgaga gctttttcct
     3781 tttgtttttt tgacatctac ttcaccatcc atgactgcat tagctttcaa tgtgtctctt
     3841 gtttcatcgt ctaaaattga aataaatgat gaagatcgtt ctttggaagg tgctatacga
     3901 tgggacataa taaataaaat aaatgcgagt tcgaaattaa acgtatgaaa ttatcgaaga
     3961 tgtcgcatat aaatagtaaa taagacgtta tccaaggcct acagccttac gaaaaaaaaa
     4021 aaaaatcaca tctcttatct tatcgatcta aatttttcag tttccttttt tcatctcgaa
     4081 gctcatcgca tgaaaaaaaa acttcgatat gtgagcgtat taagacaata gaggaaatag
     4141 tgagaacaag tgggaaaatt aaagactaat catcgaaaat atgcaagaat tctttaagtg
     4201 ctcgtatctc aaatgtagcc aaaacagtac tagcccatac aacctgatga cgagaacgtg
     4261 ctgaaagtgg aagacagtta gttacccgga caattaaaaa gtgatattaa tcaattgtat
     4321 aagtaataac ggatagctgc tatatgttta attggcatct atatagataa caagcgtgta
     4381 tagcaacaca tgtcaagtat tacatctgaa accggtaagc gaagagtgaa acgaacgtat
     4441 gaagttacaa gacagaatga caatgcagta agaatagagc cttcttcctt gggtgaggaa
     4501 gaagacaaag aggccaagga taagaactct gccttgcagc taaaacgatc acgctatgac
     4561 cccaataaag ttttttctaa tacgaaccaa ggtccagaaa agaacaattt aaaaggagaa
     4621 caacttggat cacaaaaaaa gtcatcaaag tatgatgaaa aaataacgtc aaataatgaa
     4681 cttactacaa agaagggtct actaggtgac tccgagaatg aaactaagta cgcctcttct
     4741 aattctaaat ttaacgtaga agttactcat aaaataaaga atgcaaaaga aattgacaaa
     4801 ataaacagac aacgcatgtg ggaagaacaa caattgcgaa atgctatggc ggggcaaagc
     4861 gatcaccctg atgatataac attggaaggc tctgacaagt acgattatgt gttcgacaca
     4921 gatgcaatga ttgattatac taatgaggag gacgatttac tacctgaaga gaaacttcaa
     4981 tatgaggcac gtctagcaca agctttagaa actgaggaaa agcgtatact gactatacaa
     5041 gaggcaagaa aacttttgcc tgtacatcaa tacaaggatg agctactaca agagatcaaa
     5101 aaaaatcaag ttttgattat aatgggtgaa accggttctg gtaaaaccac acagttaccc
     5161 caatatttgg tagaagatgg attcactgat cagggaaagt tacagattgc cataacacaa
     5221 ccacgtcgtg tggcggccac ttctgttgct gcaagagtag cagatgaaat gaatgttgtg
     5281 cttgggaagg aagtgggtta tcagatccgt tttgaagaca aaacaacccc aaataaaacg
     5341 gttctaaagt atatgacgga tggtatgtta ctaagagaat ttcttacaga ttccaaatta
     5401 agcaaatatt cttgtattat gattgatgaa gctcatgaac gtactctagc cacagatatc
     5461 ctgataggtt tgctgaaaga tattttgccg cagaggccca ctttaaaatt gcttatatca
     5521 tcagcaacaa tgaacgcaaa aaaattttca gagttttttg ataattgtcc cattttcaac
     5581 gttcctggga gaagatatcc tgtagatatc cactacacat tacagccgga ggcaaactat
     5641 atacatgccg ctataacaac tatttttcaa attcatacta cgcaaagttt accgggggat
     5701 atattggtgt ttttaacggg gcaagaagaa attgaaagaa caaaaaccaa actggaggag
     5761 atcatgtcta aattaggttc tagaacaaaa caaatgatta taactcctat atacgcaaat
     5821 ttaccccaag agcagcaatt gaaaattttc cagccgactc cggaaaattg taggaaagtt
     5881 gttttggcaa ctaatattgc ggaaacttca ttgaccattg atggtattag atatgtcatt
     5941 gatcctggat ttgttaagga aaattcgtac gtaccctcca ctggaatgac ccaactttta
     6001 acagttccat gctcaagagc ttcagttgat aatcgtgccg gtagggctgg tcgtgttggg
     6061 ccgggaaaat gttttagaat ttttaccaaa tggtcatatt tgcacgagtt ggaacttatg
     6121 cctaagcctg agatcacaag aacaaatctt tctaatacgg tgttattatt attatcgctt
     6181 ggcgttactg acctaataaa gtttcccctg atggataagc ccagtatccc cacattacgg
     6241 aagtctttgg aaaatttata cattttaggt gccttaaata gcaaagggac aatcactcgg
     6301 ttaggtaaaa tgatgtgcga gtttccttgt gaacctgaat ttgcaaaagt gctttataca
     6361 gctgctacgc acgaacaatg tcaaggtgta ctagaagagt gtttgaccat agtttcgatg
     6421 ctacatgaaa caccttcatt atttattggt caaaaaagag atgctgcggc aagtgtgtta
     6481 agcgaagtgg aaagtgatca catattgtat ttggaaatct tcaaccaatg gagaaactct
     6541 aaattttcca gaagttggtg tcaagaccac aaaattcaat tcaaaacgat gttgagggta
     6601 agaaatatta gaaaccaatt attcaggtgc agtgaaaagg ttggattagt tgaaaagaac
     6661 gaccaagcca ggatgaagat aggcaatata gcgggctaca taaatgctag aataactcga
     6721 tgcttcatca gcgggtttcc gatgaatatt gtacaattag gccccaccgg ctatcaaacc
     6781 atgggtaggt catcaggtgg gctgaacgtt agtgttcatc ctaccagtat acttttcgtt
     6841 aatcataagg aaaaggcgca gagaccaagc aaatatgtgc tttatcaaca attgatgcta
     6901 acgtcgaagg aatttatcag ggactgtcta gttatcccta aggaagaatg gttgatagac
     6961 atggttccac aaatctttaa agatttaatt gacgataaaa caaatagggg gaggcggtga
     7021 aggttttttg acaggtgtat gtgtattgct agaaacgcag gctccattct atatgcacgt
     7081 ttgaagatgt ggtatgcgta tgtatatata tatatatata tanngactaa agatagatag
     7141 catagatttt atcgttgtaa cttgcgataa agatcatcca agacgtctct cttaatttgc
     7201 agttcttgtt cacgttgatg gatgatatcc tgccattctg atggactttt acttaataaa
     7261 tccttggtat cttcgttttc cgatatcaaa gatttacaaa gtttcaaacg atgtctaatc
     7321 gaacctgtta aggtttccaa ttgatttgag agattgtttg gatccactag ttctagagcg
     7381 gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     7441 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7501 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7561 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7621 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7681 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7741 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7801 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7861 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7921 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7981 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8041 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8101 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8161 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8221 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8281 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8341 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8401 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8461 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8521 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8581 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8641 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8701 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8761 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8821 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8881 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8941 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9001 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9061 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9121 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9181 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9241 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9301 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9361 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9421 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9481 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9541 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9601 acctgggtcc ttttcatcac gtgctataaa aataattata atttaaattt tttaatataa
     9661 atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt
     9721 ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt
     9781 cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg
     9841 aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc
     9901 ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt
     9961 tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat
    10021 ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt
    10081 aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtcctaagaa accattatta
    10141 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
