LOCUS       sequence-182947-         461 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  This plasmid contains destabilized Cas9 and has Venus after P2A 
            sequence. This vector also contains filler sequence which required 
            to be remove for cloning of desired sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Senturk S, Shirole NH, Nowak DG, Corbo V, Pal D, Vaughan A, Tuveson 
            DA, Trotman LC, Kinney JB, Sordella R
  TITLE     Rapid and tunable method to temporally control gene editing based on
            conditional Cas9 stabilization.
  JOURNAL   Nat Commun. 2017 Feb 22;8:14370. doi: 10.1038/ncomms14370.
  PUBMED    28224990
REFERENCE   2  (bases 1 to 461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-02-22"; volume: "8:14370. doi"; pages: " 
            10.1038/ncomms14370"
COMMENT     Sequence Label: DD-Cas9 with filler sequence and Venus (EDCPV)
FEATURES             Location/Qualifiers
     source          1..461
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(54..305)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(96..117)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aatctttcac aaattttgta atccagaggt tgattgtcga cttaggatcc ttacttgtac
       61 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
      121 tcgcgcttct cgttggggtc tttgctcagg gcggactggt agctcaggta gtggttgtcg
      181 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
      241 acgccgccgt cctcgatgtt gtggcggatc ttgaagttgg ccttgatgcc gttcttctgc
      301 ttgtcggcgg tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg
      361 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg
      421 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt c
//
