LOCUS       sequence-183282-        3236 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Binds to actin and affects the structure of the cytoskeleton. At 
            high concentrations, profilin prevents the polymerization of actin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Jegou A, Niedermayer T, Orban J, Didry D, Lipowsky R, Carlier MF, 
            Romet-Lemonne G
  TITLE     Individual actin filaments in a microfluidic flow reveal the 
            mechanism of ATP hydrolysis and give insight into the properties of 
            profilin.
  JOURNAL   PLoS Biol. 2011 Sep;9(9):e1001161. doi: 
            10.1371/journal.pbio.1001161. Epub 2011 Sep 27.
  PUBMED    21980262
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-09"; volume: "9(9):e1001161. doi"; pages: " 
            10.1371/journal.pbio.1001161. Epub 2011 Sep 27"
COMMENT     Sequence Label: Profiline
FEATURES             Location/Qualifiers
     source          1..3236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     29..51
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     104..123
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        104..122
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             154..176
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(938..956)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      952..999
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1044..1062)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1130..1234
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1235..1303,1304..2095)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1453..1472)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2266..2854
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2755..2774
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3008..3025
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3040..3179
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3105..3124)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
       61 tcatcaccga aacgcgcgag gcaggatctc gatcccgcga aattaatacg actcactata
      121 gggagaccac aacggtttcc ctctagaaat aattttgttt aactttaaga aggagatata
      181 catatgggat ccatggccgg gtggaacgcc tacatcgaca acctcatggc ggacgggacc
      241 tgtcaggacg cggccatcgt gggctacaag gactcgccct ccgtctgggc cgccgtcccc
      301 gggaaaacgt tcgtcaacat cacgccagct gaggtgggtg tcctggttgg caaagaccgg
      361 tcaagttttt acgtgaatgg gctgacactt gggggccaga aatgttcggt gatccgggac
      421 tcactgctgc aggatgggga atttagcatg gatcttcgta ccaagagcac cggtggggcc
      481 cccaccttca atgtcactgt caccaagact gacaagacgc tagtcctgct gatgggcaaa
      541 gaaggtgtcc acggtggttt gatcaacaag aaatgttatg aaatggcctc ccaccttcgg
      601 cgttcccagt actgacctcg tctgtccctt ccccttcacc gctccccaca gctttgcacc
      661 cctttcctcc ccatacacac acaaaccatt ttattttttg ggccattacc ccatacccct
      721 tattgctgcc aaaaccacat gggctggggg ccagggctgg atggacagac acctccccct
      781 acccatatcc ctcccgtgtg tggttggaaa acttttgttt tttggggttt tttttttctg
      841 aataaaaaag attctactaa aaaaaaaaaa aaaaaaaaaa ggaattccga tccggctgct
      901 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
      961 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
     1021 ggataattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     1081 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     1141 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     1201 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     1261 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     1321 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     1381 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     1441 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     1501 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     1561 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     1621 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     1681 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     1741 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg
     1801 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     1861 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     1921 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     1981 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     2041 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     2101 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     2161 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     2221 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2281 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2341 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2401 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2461 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2521 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2581 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2641 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2701 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2761 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2821 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2881 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2941 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     3001 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     3061 tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat
     3121 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     3181 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggca
//
