LOCUS       sequence-183652-        1021 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Mammalian expression of E. coli LF82_323 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_323
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    562..696
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(586..605)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     640..659
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(807..823)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(807..823)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(820..842)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    831..847
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(855..861,862..879,880..885))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    900..921
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cggcatggac gagctgtaca agcgaattgg tggagggggc ggttccggtg gcggaggttc
       61 ccgaattcgt cgacggagat ctgatatgat atcgatcagt gttgccgatg gtgcgggact
      121 acagaactac gatgccgtgc ttgaacatat ttataacacg catcgtgaaa cgaatacggt
      181 gaaagagagc tggctcaagg ctatgcgtaa acgcgaagcg gaatatccga caggaataga
      241 actggaaggt tattctatcg ctataccaca ctgtgatagt gaacatgcga gtcaaccggc
      301 tatttatatc atccgtctgc cagaagcaat acctgtgaac agggctgatg aagatggaat
      361 attgtgggtc agactcgtcg ttaatcttat tgttacggat cctgctgacc agcttcgatt
      421 gttaaagtca ttatttaacc atctccaaaa tgaagaattt taccaaaact tactgacact
      481 tcccgtcgag gatgcaaaag cactttttgt gtccaaaata atccaatgaa tcggtaccct
      541 cgagaagctt gcggccgcgg ggatccagac atgataagat acattgatga gtttggacaa
      601 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
      661 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
      721 atgtttcagg ttcaggggga ggtgtgggag gttttttcgg atcctctaga gtcgatctgc
      781 aggcatgcta gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      841 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      901 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      961 ctgtcntgnn agctgcattn atgaatcggn naacgcgcgg ggnagaggcg gttngcgtan
     1021 t
//
