LOCUS       sequence-183661-        1022 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Mammalian expression of E. coli LF82_689 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_689
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    519..653
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(543..562)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     597..616
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(764..780)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(764..780)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(777..799)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    788..804
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(812..818,819..836,837..842))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    857..878
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(995..1012)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcngnatgg ncgagctgta cnagcgaatt ggtggagggg gcggttccgg tggcggaggt
       61 tcccgaattc gtcgacggag atctgatatg atgcgattca ggaacaccgt gctggacgtt
      121 gtggagggta atatctcgga taacggcgtg ttgtttgaag cgccaccgca gggtaatccg
      181 cgaattagtc agtataatca tgcacaactg gcggaattat gccgccagat ccgtcagcgg
      241 gtaggggaga aggcgacatt tacctgttcg cctcatcgtg tggccgggca taactgcctg
      301 gctgtgcagg tgctgggtat gaccggaacg gttaacctgc tgctcactgt caccgacagt
      361 ctgcgctggc ctgcggcaga ggattacgaa catggggtgc gctggtatat caatttggtt
      421 gatgctgtgg atgtgagtta tttagtattc gaactctata gctcattgac tttattaggt
      481 atttgaatcg gtaccctcga gaagcttgcg gccgcgggga tccagacatg ataagataca
      541 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
      601 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
      661 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttcggatc
      721 ctctagagtc gatctgcang catgctagct tggcgtaatc atggtcatag ctgtttcctg
      781 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
      841 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      901 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      961 naggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     1021 nc
//
