LOCUS       sequence-183702-         790 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Mammalian expression of E. coli LF82_770 CDS fused to mEmerald.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Collins A, Huett A
  TITLE     A multi-phenotypic imaging screen to identify bacterial effectors by
            exogenous expression in a HeLa cell line.
  JOURNAL   Sci Data. 2018 May 15;5:180081. doi: 10.1038/sdata.2018.81.
  PUBMED    29762554
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Data.";
            date: "2018-05-15"; volume: "5:180081. doi"; pages: " 
            10.1038/sdata.2018.81"
COMMENT     Sequence Label: pCMV_mEm_4GS_LF82_770
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    308..442
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(332..351)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     386..405
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(553..569)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(553..569)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(566..588)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    577..593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(601..607,608..625,626..631))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    647..668
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 atttaacact caaatttgcc gacagggccg acttttcggc ctttatggat agcactggct
       61 attatgatga cgagtcgatg caggatgata ttcttattga cgtgataggt aacgtgtaca
      121 aagaaaccgg agaactgact gaagatggcg aaccggtatg tgttaaggaa gacggatatt
      181 ttgtaaacgt gcgcatcatt aatgattcgc aaatatcgtc attattcgat gaatacgtgg
      241 ttgctgttga gcatcaactt cgtggctgga tgtgaatcgg taccctcgag aagcttgcgg
      301 ccgcggggat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
      361 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
      421 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
      481 gggggaggtg tgggaggttt tttcggatcc tctagagtcg atctgcaggc atgctagctt
      541 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      601 caacatacga gccggaagca taaagtgtaa agcctggggg tgcctaatga gtgagctaac
      661 tcacattaat ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      721 ctgcattatg aatcggccaa cgcgcgggga gaggcggttt tgcgtattgg gcgctcttcc
      781 gcttcctccg
//
