LOCUS       sequence-18422-e         642 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Wong ET, Kolman JL, Li YC, Mesner LD, Hillen W, Berens C, Wahl GM
  TITLE     Reproducible doxycycline-inducible transgene expression at specific 
            loci generated by Cre-recombinase mediated cassette exchange.
  JOURNAL   Nucleic Acids Res. 2005  . 33(17):e147.
  PUBMED    16204450
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2005  . 33(17):e147."
COMMENT     Sequence Label: RV-L3-HyTK-2L
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..58
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(116..136)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cctctagaga accccttcat cgagataact tcgtataaag tctcctatac gaagttatat
       61 cgatggccct cattatttgt agagctcatc catgccatgt gtaatcccag cagcagttac
      121 aaactcaaga aggaccatgt ggtcacgctt ttcgttggga tctttcgaaa gggcagattg
      181 tgtcgacagg taatggttgt ctggtaaaag gacagggcca tcgccaattg gagtattttg
      241 ttgataatgg tctgctagtt gaacggatcc atcttcaatg ttgtgacgaa ttttgaagtt
      301 agctttgatt ccattctttt gtttgtctgc cgtgatgtat acattgtgtg agttatagtt
      361 gtactcgagt ttgtgtccga gaatgtttcc atcttcttta aaatcaatac cttttaactc
      421 gatacgatta acaagggtat caccttcaaa cttgacttca gcacgcgtct tgtagttccc
      481 gtcatctttg aaagatatag tgcgttcctg tacataacct tcgggcatgg cactcttgaa
      541 aaagtcatgc cgtttcatat gatccggata acgggaaaag cattgaacac cataagagaa
      601 agtagtgaca agtgttggcc atggaacagg tagttttcca gt
//
