LOCUS       sequence-18485-e         586 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Tet-regulated (Tet-off) lentiviral vector for transgene (CAG 
            promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM 
            (Addgene#12247)) - 2nd generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
  TITLE     A versatile tool for conditional gene expression and knockdown.
  JOURNAL   Nat Methods. 2006 Feb;3(2):109-16. doi: 10.1038/nmeth846
  PUBMED    16432520
REFERENCE   2  (bases 1 to 586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-02"; volume: "3"; issue: "2"; pages: 
            "109-16"
COMMENT     Sequence Label: pLVCT-rtTR-KRAB-2SM2
FEATURES             Location/Qualifiers
     source          1..586
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    104..221
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(280..299)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(361..383)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ctcctcgccc ttgctcacca tggtggcgac cggtagcgct aggacgcgta agcttaggcc
       61 tggatcccgt agtttaaacc tcgaggctag tctcgtgatc gataaaattt tgaatttttg
      121 taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt
      181 cccctgcact gtacccccca atcccccctt ttcttttaaa agttaaccga taccgtcgag
      241 attaattaaa tttaattctt tgccaaaatg atgagacagc acaataacca gcacgttgcc
      301 caggagctgt aggaaaaaga agaaggcatg aacatggtta gcagaggctc tagagccgcc
      361 ggtcacacgc cagaagccga accccgccct gccccgtccc ccccgaaggc agccgtcccc
      421 ccgcggacag ccccgaggct ggagagggag aaggggacgg cggcgcggcg acgcacgaag
      481 gccctccccg cccatttcct tcctgccggc gccgcaccgc ttcgccccgc gcccgctana
      541 gggggtgcng cggcgcctcc cagatttcng ctccncacag atttgg
//
