LOCUS       sequence-189140-         570 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  3rd generation lentiviral gRNA plasmid, non-targeting control gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437."
COMMENT     Sequence Label: non-targeting control gRNA (BRDN0001147185)
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        177..252
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    308..425
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aggtaccnnt tncgnggacg ntagaaagta ataatttctt gggtagttng cagttttaan
       61 attacngttt taaaatggac tatcntatgc ttaccgtaac ttgnaagtat ttcgatttct
      121 tggctttata tatcttgtgg aaaggacgaa acaccgcgta gtaaatatct agctaagttt
      181 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca
      241 ccgagtcggt gcttttttaa gcttggcgta actagatctt gagacaaatg gcagtattcn
      301 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcangggaa agaatagtag
      361 acataatagc aacagacata caaactaaag aattaccaaa acaaattacn aaaattcaaa
      421 attttcgggt ttattacagg ggacagcana gatccacttt gggcgccggc tcgagggggc
      481 ccgggtgcaa agatgggata angtttttaa acagaggagg aaatctttnc agcttaatgg
      541 naccttncta gggnctttgn aaaggagtng
//
