LOCUS       sequence-189515-        8469 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses CDA1-BE3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Komor AC, Zhao KT, Packer MS, Gaudelli NM, Waterbury AL, Koblan LW, 
            Kim YB, Badran AH, Liu DR
  TITLE     Improved base excision repair inhibition and bacteriophage Mu Gam 
            protein yields C:G-to-T:A base editors with higher efficiency and 
            product purity.
  JOURNAL   Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. 
            eCollection 2017 Aug.
  PUBMED    28875174
REFERENCE   2  (bases 1 to 8469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2017-08-30"; volume: "3(8):eaao4774. doi"; pages: " 
            10.1126/sciadv.aao4774. eCollection 2017 Aug"
COMMENT     Sequence Label: CDA1-BE3
FEATURES             Location/Qualifiers
     source          1..8469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             409..1032
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
     CDS             join(1081..4479,4480..4482,4483..5079,5080,5081..5083,
                     5084..5085,5086..5088,5089..5181)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1081..1104,1105..1107,1108..5181)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5194..5442
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5455..5475
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5484..5501
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5524..5541)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5530..5754
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5825..5841)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5825..5841)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5838..5860)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5849..5865
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5873..5879,5880..5897,5898..5903))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5918..5939
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6056..6073)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6227..6815)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6307..6326)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6986..7777,7778..7846))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7609..7628
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7847..7951)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8030..8049)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8221..8469,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gacagacgct
      421 gaatatgtta ggatccatga aaaactggat atctatacat ttaagaagca gttcttcaat
      481 aacaaaaagt cagtatctca cagatgctat gtcctgttcg aactcaagag aagaggagaa
      541 aggcgggcct gtttctgggg gtacgcggtt aataaacccc agtccgggac cgagaggggg
      601 attcacgccg agatcttttc aattaggaag gttgaagagt atcttcgcga caatcccggt
      661 cagttcacaa ttaactggta cagctcctgg agcccttgcg ctgattgcgc cgagaaaata
      721 ctcgaatggt acaaccagga gttgagaggc aatggccaca ctctcaagat ttgggcttgc
      781 aagctttact acgagaagaa cgcgagaaat cagattggct tgtggaacct cagggacaac
      841 ggggtcgggt tgaatgttat ggtgtccgaa cattaccagt gctgtagaaa gatcttcatt
      901 cagtccagtc acaatcagct gaacgagaac agatggctgg agaaaacact gaaacgggca
      961 gagaaaaggc gctcagagct gagtatcatg atccaggtca aaatcctgca tacaaccaaa
     1021 agcccggctg taagcggcag cgagactccc gggacctcag agtccgccac acccgaaagt
     1081 gataaaaagt attctattgg tttagccatc ggcactaatt ccgttggatg ggctgtcata
     1141 accgatgaat acaaagtacc ttcaaagaaa tttaaggtgt tggggaacac agaccgtcat
     1201 tcgattaaaa agaatcttat cggtgccctc ctattcgata gtggcgaaac ggcagaggcg
     1261 actcgcctga aacgaaccgc tcggagaagg tatacacgtc gcaagaaccg aatatgttac
     1321 ttacaagaaa tttttagcaa tgagatggcc aaagttgacg attctttctt tcaccgtttg
     1381 gaagagtcct tccttgtcga agaggacaag aaacatgaac ggcaccccat ctttggaaac
     1441 atagtagatg aggtggcata tcatgaaaag tacccaacga tttatcacct cagaaaaaag
     1501 ctagttgact caactgataa agcggacctg aggttaatct acttggctct tgcccatatg
     1561 ataaagttcc gtgggcactt tctcattgag ggtgatctaa atccggacaa ctcggatgtc
     1621 gacaaactgt tcatccagtt agtacaaacc tataatcagt tgtttgaaga gaaccctata
     1681 aatgcaagtg gcgtggatgc gaaggctatt cttagcgccc gcctctctaa atcccgacgg
     1741 ctagaaaacc tgatcgcaca attacccgga gagaagaaaa atgggttgtt cggtaacctt
     1801 atagcgctct cactaggcct gacaccaaat tttaagtcga acttcgactt agctgaagat
     1861 gccaaattgc agcttagtaa ggacacgtac gatgacgatc tcgacaatct actggcacaa
     1921 attggagatc agtatgcgga cttatttttg gctgccaaaa accttagcga tgcaatcctc
     1981 ctatctgaca tactgagagt taatactgag attaccaagg cgccgttatc cgcttcaatg
     2041 atcaaaaggt acgatgaaca tcaccaagac ttgacacttc tcaaggccct agtccgtcag
     2101 caactgcctg agaaatataa ggaaatattc tttgatcagt cgaaaaacgg gtacgcaggt
     2161 tatattgacg gcggagcgag tcaagaggaa ttctacaagt ttatcaaacc catattagag
     2221 aagatggatg ggacggaaga gttgcttgta aaactcaatc gcgaagatct actgcgaaag
     2281 cagcggactt tcgacaacgg tagcattcca catcaaatcc acttaggcga attgcatgct
     2341 atacttagaa ggcaggagga tttttatccg ttcctcaaag acaatcgtga aaagattgag
     2401 aaaatcctaa cctttcgcat accttactat gtgggacccc tggcccgagg gaactctcgg
     2461 ttcgcatgga tgacaagaaa gtccgaagaa acgattactc catggaattt tgaggaagtt
     2521 gtcgataaag gtgcgtcagc tcaatcgttc atcgagagga tgaccaactt tgacaagaat
     2581 ttaccgaacg aaaaagtatt gcctaagcac agtttacttt acgagtattt cacagtgtac
     2641 aatgaactca cgaaagttaa gtatgtcact gagggcatgc gtaaacccgc ctttctaagc
     2701 ggagaacaga agaaagcaat agtagatctg ttattcaaga ccaaccgcaa agtgacagtt
     2761 aagcaattga aagaggacta ctttaagaaa attgaatgct tcgattctgt cgagatctcc
     2821 ggggtagaag atcgatttaa tgcgtcactt ggtacgtatc atgacctcct aaagataatt
     2881 aaagataagg acttcctgga taacgaagag aatgaagata tcttagaaga tatagtgttg
     2941 actcttaccc tctttgaaga tcgggaaatg attgaggaaa gactaaaaac atacgctcac
     3001 ctgttcgacg ataaggttat gaaacagtta aagaggcgtc gctatacggg ctggggacga
     3061 ttgtcgcgga aacttatcaa cgggataaga gacaagcaaa gtggtaaaac tattctcgat
     3121 tttctaaaga gcgacggctt cgccaatagg aactttatgc agctgatcca tgatgactct
     3181 ttaaccttca aagaggatat acaaaaggca caggtttccg gacaagggga ctcattgcac
     3241 gaacatattg cgaatcttgc tggttcgcca gccatcaaaa agggcatact ccagacagtc
     3301 aaagtagtgg atgagctagt taaggtcatg ggacgtcaca aaccggaaaa cattgtaatc
     3361 gagatggcac gcgaaaatca aacgactcag aaggggcaaa aaaacagtcg agagcggatg
     3421 aagagaatag aagagggtat taaagaactg ggcagccaga tcttaaagga gcatcctgtg
     3481 gaaaataccc aattgcagaa cgagaaactt tacctctatt acctacaaaa tggaagggac
     3541 atgtatgttg atcaggaact ggacataaac cgtttatctg attacgacgt cgatcacatt
     3601 gtaccccaat cctttttgaa ggacgattca atcgacaata aagtgcttac acgctcggat
     3661 aagaaccgag ggaaaagtga caatgttcca agcgaggaag tcgtaaagaa aatgaagaac
     3721 tattggcggc agctcctaaa tgcgaaactg ataacgcaaa gaaagttcga taacttaact
     3781 aaagctgaga ggggtggctt gtctgaactt gacaaggccg gatttattaa acgtcagctc
     3841 gtggaaaccc gccaaatcac aaagcatgtt gcacagatac tagattcccg aatgaatacg
     3901 aaatacgacg agaacgataa gctgattcgg gaagtcaaag taatcacttt aaagtcaaaa
     3961 ttggtgtcgg acttcagaaa ggattttcaa ttctataaag ttagggagat aaataactac
     4021 caccatgcgc acgacgctta tcttaatgcc gtcgtaggga ccgcactcat taagaaatac
     4081 ccgaagctag aaagtgagtt tgtgtatggt gattacaaag tttatgacgt ccgtaagatg
     4141 atcgcgaaaa gcgaacagga gataggcaag gctacagcca aatacttctt ttattctaac
     4201 attatgaatt tctttaagac ggaaatcact ctggcaaacg gagagatacg caaacgacct
     4261 ttaattgaaa ccaatgggga gacaggtgaa atcgtatggg ataagggccg ggacttcgcg
     4321 acggtgagaa aagttttgtc catgccccaa gtcaacatag taaagaaaac tgaggtgcag
     4381 accggagggt tttcaaagga atcgattctt ccaaaaagga atagtgataa gctcatcgct
     4441 cgtaaaaagg actgggaccc gaaaaagtac ggtggcttcg atagccctac agttgcctat
     4501 tctgtcctag tagtggcaaa agttgagaag ggaaaatcca agaaactgaa gtcagtcaaa
     4561 gaattattgg ggataacgat tatggagcgc tcgtcttttg aaaagaaccc catcgacttc
     4621 cttgaggcga aaggttacaa ggaagtaaaa aaggatctca taattaaact accaaagtat
     4681 agtctgtttg agttagaaaa tggccgaaaa cggatgttgg ctagcgccgg agagcttcaa
     4741 aaggggaacg aactcgcact accgtctaaa tacgtgaatt tcctgtattt agcgtcccat
     4801 tacgagaagt tgaaaggttc acctgaagat aacgaacaga agcaactttt tgttgagcag
     4861 cacaaacatt atctcgacga aatcatagag caaatttcgg aattcagtaa gagagtcatc
     4921 ctagctgatg ccaatctgga caaagtatta agcgcataca acaagcacag ggataaaccc
     4981 atacgtgagc aggcggaaaa tattatccat ttgtttactc ttaccaacct cggcgctcca
     5041 gccgcattca agtattttga cacaacgata gatcgcaaac gatacacttc taccaaggag
     5101 gtgctagacg cgacactgat tcaccaatcc atcacgggat tatatgaaac tcggatagat
     5161 ttgtcacagc ttgggggtga ctctggtggt tctactaatc tgtcagatat tattgaaaag
     5221 gagaccggta agcaactggt tatccaggaa tccatcctca tgctcccaga ggaggtggaa
     5281 gaagtcattg ggaacaagcc ggaaagcgat atactcgtgc acaccgccta cgacgagagc
     5341 accgacgaga atgtcatgct tctgactagc gacgcccctg aatacaagcc ttgggctctg
     5401 gtcatacagg atagcaacgg tgagaacaag attaagatgc tctctggtgg ttctcccaag
     5461 aagaagagga aagtctaacc ggtcatcatc accatcacca ttgagtttaa acccgctgat
     5521 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     5581 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     5641 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     5701 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     5761 aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc ttggcgtaat
     5821 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     5881 gagccggaag cataaagtgt aaagcctagg gtgcctaatg agtgagctaa ctcacattaa
     5941 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     6001 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6061 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6121 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6181 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6241 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6301 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6361 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6421 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6481 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6541 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6601 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6661 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6721 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6781 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6841 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     6901 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6961 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7021 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7081 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7141 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7201 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7261 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7321 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7381 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7441 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7501 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7561 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7621 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7681 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7741 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7801 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     7861 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     7921 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     7981 tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt acaatctgct
     8041 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     8101 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     8161 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     8221 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     8281 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     8341 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     8401 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     8461 aagtgtatc
//
