LOCUS       sequence-189578-        1942 bp    DNA     circular SYN 15-APR-2025
DEFINITION  EMMA toolkit Assembly connector L-Y.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1918 HC_Kan_conn_L-Y
FEATURES             Location/Qualifiers
     source          1..1942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      281..310
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      332..919
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     820..839
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(931..958)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(982..1797)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1705..1724
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1798..1900)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(1901..1932)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 gaggtcaggt atgatttaaa tggtcagtat tgagcgatat ctagagaatt cgtcagaagt
       61 tggtgagttc agagcgtctc acgtctatga tgaggatgtt ggtggagagc atgtggagga
      121 agtggatagg gaaggttgta gagtagatcc ggttgaagtg atgaggatag gaggaggact
      181 gcgagacggt gaatgaggag attgagctaa cgaattcaag cttgatatca ttcaggacga
      241 gcctcagact ccagcgtaac tggactgcaa tcaactcact ggctcacctt cacgggtggg
      301 cctttcttcg gtagaaaatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
      361 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
      421 taccaactct ttttccgagg taactggctt cagcagagcg cagataccaa atactgttct
      481 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
      541 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
      601 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
      661 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      721 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      781 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      841 tagtcctgtc gggtttcgcc acctctgact tgagcatcga tttttgtgat gctcgtcagg
      901 ggggcggagc ctatggaaaa acgccagcaa cgcagaaagg cccacccgaa ggtgagccag
      961 gtgattacat ttgggccctc attagaaaaa ctcatcgagc atcaagtgaa actgcaattt
     1021 attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga
     1081 aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac
     1141 tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga
     1201 gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat gcatttcttt
     1261 ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg caccaaccaa
     1321 accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc cgttaaaagg
     1381 acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat
     1441 attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc ctgggatcgc
     1501 agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg
     1561 cataaattcc gtcagccagt ttagcctgac catctcatct gtaacatcat tggcaacgct
     1621 acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat
     1681 tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc
     1741 catgttggaa tttaatcgcg gcctcgagca agacgtttcc cgttgaatat ggctcatttt
     1801 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
     1861 ttcattatgg tgaaagttgg aacctcttac gtgccgatca agtcaaaagc ctccggtcgg
     1921 aggcttttga ctttctgcta tg
//
