LOCUS       sequence-190067-        7769 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, 
            Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, 
            Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, 
            Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, 
            Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, 
            Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, 
            Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, 
            Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Munger K, 
            Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M
  TITLE     Interpreting cancer genomes using systematic host network 
            perturbations by tumour virus proteins.
  JOURNAL   Nature. 2012 Jul 26;487(7408):491-5.
  PUBMED    22810586
REFERENCE   2  (bases 1 to 7769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2012-07-26"; volume: "487(7408)"; pages: "491-5"
COMMENT     Sequence Label: MSCV-N EBNA1
FEATURES             Location/Qualifiers
     source          1..7769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(364..388)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(404..430)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(443..466)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      466..475
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(488..904)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(504..520)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(536..558)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    971..1312
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     LTR             1376..1892
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(2092..2111)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2239..2261)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2455..2474)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2574..2596
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2634..2652)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2720..2824
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2825..2893,2894..3685)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3043..3062)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3856..4444
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4345..4364
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4598..4615
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4732..4753
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4768..4773,4774..4791,4792..4798)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4806..4822
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     LTR             4984..5498
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     CDS             complement(5574..6173)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5657..5677)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6154..6173
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    6185..6737
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6187..6754
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6347..6366)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     6576..6593
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    6767..6791
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctcct gctcctgccc
       61 ctcctgctcc tgcccctccc gctcctgctc ctgctcctgt tccaccgtgg gtccctttgc
      121 agccaatgca acttggacgt ttttggggtc tccggacacc atctctatgt cttggccctg
      181 atcctgagcc gcccggggct cctggtcttc cgcctcctcg tcctcgtcct cttccccgtc
      241 ctcgtccatg gttatcaccc cctcttcttt gaggtccact gccgccggag ccttctggtc
      301 cagatgtgtc tcccttctct cctaggccat ttccaggtcc tgtacctggc ccctcgtcag
      361 acatgccaac ttttttgtac aaacttgttt gataacctcg agcagcgtaa tctggaacat
      421 cgtatgggta tcctccatcg agcttgtcat cgtcatcctt gtagtccatg gtggcagatc
      481 taattccggc gcctagagaa ggagtgaggg ctggataaag ggaggatcga ggcggggtcg
      541 aacgaggagg ttcaaggggg agagacgggg cggatggagg aagaggaggc ggaggcttag
      601 ggtgtacaaa gggcttgacc cagggagggg ggtcaaaagc caaggcttcc caggtcacga
      661 tgtaggggac ctggtctggg tgtccatgcg ggccaggtga aaagaccttg atcttaacct
      721 gggtgatgag gtctcggtta aaggtgccgt ctcgcggcca tccgacgtta aaggttggcc
      781 attctgcaga gcagaaggta acccaacgtc tcttcttgac atctaccgac tggttgtgag
      841 cgatccgctc gacatctttc cagtgaccta aggtcaaact taagggagtg gtaacagtct
      901 ggccctaatt ttcagacaaa tacagaaaca cagtcagaca gagacaacac agaacgatgc
      961 tgcagcgctg cagcagacaa gacgcgcggc ttcggttcca aaccgaaagc aaaaattcag
     1021 acggaggcgg gaactgtttt aggttctcgt ctcctaccag aaccacatat cctgacgggg
     1081 tcggattcca catcgactcc cttcctcagg tcgggccaca aaaacggccc ccaaagtccc
     1141 tgggacgtct cccagggttg cggccgggtg ttcagaactc gtcagttcca ccacgggtcc
     1201 gccagataca gagctagtta gctaactagt acagacgcag gcgcaaacat tagatgccgg
     1261 cacaaacacg cacaaagaca gagacagaca cgaaacgacc gctggccagc ttacctcccg
     1321 gcgggggggt cggtggtccc tgggcagggg tctccaaatc tcggtggaac ctccaaatga
     1381 aagacccccg aggtgggcag tcaatcaatc tgaggagacc ctcccaagga tcagcgagtc
     1441 cacgattcgg atgcaaacag caagaggctt tattgggaat acgggtaccc gggcgacgca
     1501 gtctatcgga ggactggcgc gccgagtgag gggttgtggg ctcttttatt gagctcgggg
     1561 agcagaagcg cgcgaacaga agcgagaagc gaactgattg gttagttcaa ataaggcaca
     1621 gggtcatttc aggtccttgg ggcaccctgg aaacatctga tggttctcta gaaactgctg
     1681 agggcgggac cgcatctggg gaccatctgt tcttggccct gagccggggc aggaactgct
     1741 taccacagat atcctgtttg gcccatattc tgctgtctct ctgttcctaa ccttgatctg
     1801 aacttctcta ttctcagtta tgtattttcc atgccttgca aaatggcgtt acttaagcta
     1861 gcttgccaaa cctacaggtg gggtctttca ttcccccctt tttctggaga ctaaataaaa
     1921 tcttttattt tatctatggc tcgtactcta taggcttcag ctggtgatat tgttgagtca
     1981 aaactagagc ctggaccact gatatcctgt ctttaacaaa ttggactaat cgcctctacg
     2041 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
     2101 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
     2161 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg
     2221 caccattcct tgcgccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     2281 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     2341 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     2401 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     2461 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     2521 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     2581 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     2641 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     2701 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     2761 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2821 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2881 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2941 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3001 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3061 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3121 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     3181 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3241 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     3301 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3361 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3421 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3481 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3541 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3601 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3661 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     3721 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     3781 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3841 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3901 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3961 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4021 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4081 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4141 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4201 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4261 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4321 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4381 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4441 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4501 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4561 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     4621 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     4681 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     4741 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     4801 tggaattgtg agcggataac aatttcacaa ttctccccag ctctttctgc gctgtccata
     4861 atgaactatt tcaggagtat tggagtatgt ggattcaaaa cagtggctaa ttgagtcagg
     4921 attacttaat attcgactcc taccctcaga atgttgttct ccaacttcaa gaaactgtta
     4981 cccatgaaag acccccgctg acgggtagtc aatcactcag aggagaccct cccaaggaac
     5041 agcgagacca caagtcggat gcaactgcaa gagggtttat tggatacacg ggtacccggg
     5101 cgacgcagtc tatcggagga ctggcgcgcc gagtgagggg ttgtgggctc ttttattgag
     5161 ctcggggagc agaagcgcgc gaacagaagc gagaagcgaa ctgattggtt agttcaaata
     5221 aggcacaggg tcatttcagg tccttggggc accctggaaa catctgatgg ttctctagaa
     5281 actgctgagg gcgggaccgc atctggggac catctgttct tggccctgag ccggggcagg
     5341 aactgcttac cacagatatc ctgtttggcc catattctgc tgtctctctg ttcctaacct
     5401 tgatctgaac ttctctattc tcagttatgt attttccatg ccttgcaaaa tggcgttact
     5461 taagctagct tgccaaacct acaggtgggg tctttcattc cccccttttt ctggagacta
     5521 aataaaatct tttattttat cgataagctt ggctgcaggt cgacatcgat gcatcaggca
     5581 ccgggcttgc gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg
     5641 acggtgaagc cgagccgctc gtagaagggg aggttgcggg gcgcggaggt ctccaggaag
     5701 gcgggcaccc cggcgcgctc ggccgcctcc actccgggga gcacgacggc gctgcccaga
     5761 cccttgccct ggtggtcggg cgagacgccg acggtggcca ggaaccacgc gggctccttg
     5821 ggccggtgcg gcgccaggag gccttccatc tgttgctgcg cggccagccg ggaaccgctc
     5881 aactcggcca tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac gctctccggc
     5941 gtggtccaga ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc
     6001 ccgacgcgcg tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg gcggtccgga
     6061 tcgacggtgt ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc
     6121 ctggggacgt cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt catggttgtg
     6181 gccatattat catcgtgttt ttcaaaggaa aaccacgtcc ccgtggttcg gggggcctag
     6241 acgttttttt aacctcgact aaacacatgt aaagcatgtg caccgaggcc ccagatcaga
     6301 tcccatacaa tggggtacct tctgggcatc cttcagcccc ttgttgaata cgcttgagga
     6361 gagccatttg actctttcca caactatcca actcacaacg tggcactggg gttgtgccgc
     6421 ctttgcaggt gtatcttata cacgtggctt ttggccgcag aggcacctgt cgccaggtgg
     6481 ggggttccgc tgcctgcaaa gggtcgctac agacgttgtt tgtcttcaag aagcttccag
     6541 aggaactgct tccttcacga cattcaacag accttgcatt cctttggcga gaggggaaag
     6601 acccctagga atgctcgtca agaagacagg gccaggtttc cgggccctca cattgccaaa
     6661 agacggcaat atggtggaaa ataacatata gacaaacgca caccggcctt attccaagcg
     6721 gcttcggcca gtaacgttag gggggggggg cggaattcgt tatcgaacca ctttgtacaa
     6781 gaaagttggc tcctgccctt cctcaccctc atctccatca cctccttcat ctccgtcatc
     6841 tccgtcatca ccctccgcgg cagccccttc caccataggt ggaaaccagg gaggcaaatc
     6901 tactccatcg tcaaagctgc acacagtcac cctgatattg caggtaggag cgggctttgt
     6961 cataacaagg tccttaatcg catccttcaa aacctcagca aatatatgag tttgtaaaaa
     7021 gaccatgaaa taacagacaa tggactccct tagcgggcca ggttgtgggc cgggtccagg
     7081 ggccattcca aaggggagac gactcaatgg tgtaagacga cattgtggaa tagcaagggc
     7141 agttcctcgc cttaggttgt aaagggaggt cttactacct ccatatacga acacaccggc
     7201 gacccaagtt ccttcgtcgg tagtcctttc tacgtgactc ctagccagga gagctcttaa
     7261 accttctgca atgttctcaa atttcgggtt ggaacctcct tgaccacgat gctttccaaa
     7321 ccaccctcct tttttgcgcc tgcctccatc accctgaccc cggggtccag tgcttgggcc
     7381 ttctcctggg tcatctgcgg ggccctgctc tatcgctccc gggggcacgt caggctcacc
     7441 atctgggcca ccttcttggt ggtattcaaa ataatcggct tcccctacag ggtggaaaaa
     7501 tggccttcta cctggagggg gcctgcgcgg tggagacccg gatgatgatg actgactact
     7561 gggactcctg ggcctctttt ctccacgtcc acgacctctc cccctggctc tttcacgact
     7621 tccccccctg gctctttcac gtcctctacc ccggcggcct ccactacctc ctcgaccccg
     7681 gcctccacta cctcctcgac cccggcctcc actgcctcct cgaccccggc ctccacctcc
     7741 tgctcctgcc cctcctgctc ctgcccctc
//
