LOCUS       sequence-190070-        7802 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Mammalian Gateway-compatible expression vector backbone with an 
            N-terminal Nanoluc luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7802)
  TITLE     Taipale Lab plasmids
REFERENCE   2  (bases 1 to 7802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3.1-Nanoluc-ccdB
FEATURES             Location/Qualifiers
     source          1..7802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     13..34
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(153..173)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        158..487
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      338..473
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     400..419
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             536..1561
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             536..1561
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    1691..1812
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1728..1747)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1782..1801
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1861..1877)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1861..1877)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1874..1896)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1885..1901
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1909..1915,1916..1933,1934..1939))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1954..1975
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2092..2109)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2263..2851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2343..2362)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3022..3813,3814..3882))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3645..3664
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3883..3987)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4062..4081)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4253..4632
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4633..4836
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4787..4807
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4833..4857
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4923..4932
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4929..5441
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     protein_bind    5487..5611
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5636..5641,5642..5659,5660..5666)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5720..6379
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5786..5805)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6721..7026
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6905..6924
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(7067..7191)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7242..7259)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7248..7472
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(7518..7802,1..144)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7605..7624)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
       61 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
      121 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
      181 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
      241 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      301 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
      361 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
      421 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
      481 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgatgaa
      541 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
      601 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
      661 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
      721 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
      781 attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
      841 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat
      901 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
      961 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
     1021 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     1081 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     1141 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
     1201 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     1261 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
     1321 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     1381 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     1441 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
     1501 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata
     1561 gcacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     1621 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     1681 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     1741 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     1801 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     1861 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     1921 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     1981 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     2041 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     2101 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2161 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2221 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2281 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2341 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2401 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2461 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2521 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2581 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2641 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2701 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2761 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2821 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2881 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2941 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3001 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3061 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3121 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     3181 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3241 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3301 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3361 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3421 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3481 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3541 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3601 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     3661 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     3721 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     3781 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     3841 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     3901 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     3961 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     4021 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
     4081 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     4141 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     4201 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
     4261 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4321 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4381 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4441 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4501 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4561 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4621 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4681 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4741 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4801 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     4861 tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta gaggatccag
     4921 ccgccaccat ggtcttcaca ctcgaagatt tcgttgggga ctggcgacag acagccggct
     4981 acaacctgga ccaagtcctt gaacagggag gtgtgtccag tttgtttcag aatctcgggg
     5041 tgtccgtaac tccgatccaa aggattgtcc tgagcggtga aaatgggctg aagatcgaca
     5101 tccatgtcat catcccgtat gaaggtctga gcggcgacca aatgggccag atcgaaaaaa
     5161 tttttaaggt ggtgtaccct gtggatgatc atcactttaa ggtgatcctg cactatggca
     5221 cactggtaat cgacggggtt acgccgaaca tgatcgacta tttcggacgg ccgtatgaag
     5281 gcatcgccgt gttcgacggc aaaaagatca ctgtaacagg gaccctgtgg aacggcaaca
     5341 aaattatcga cgagcgcctg atcaaccccg acggctccct gctgttccga gtaaccatca
     5401 acggagtgac cggctggcgg ctgtgcgaac gcattctggc gagtggcggc cgctcgagtg
     5461 gctcgggctc gacctcgggc tcgggcacaa gtttgtacaa aaaagctgaa cgagaaacgt
     5521 aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag actacataat
     5581 actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac
     5641 actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc
     5701 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
     5761 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
     5821 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
     5881 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg
     5941 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
     6001 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
     6061 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
     6121 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
     6181 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
     6241 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     6301 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
     6361 gtggcagggc ggggcgtaaa gatctggatc cggcttacta aaagccagat aacagtatgc
     6421 gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat
     6481 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
     6541 cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga
     6601 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
     6661 aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa
     6721 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
     6781 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
     6841 ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
     6901 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
     6961 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
     7021 atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat
     7081 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
     7141 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg ttgctctata
     7201 gtaactcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     7261 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     7321 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     7381 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     7441 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     7501 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     7561 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     7621 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     7681 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     7741 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     7801 ca
//
