LOCUS       sequence-190176-       12779 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and 
            Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the 
            control of GAL1/GAL2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12779)
  AUTHORS   Peng B, Plan MR, Carpenter A, Nielsen LK, Vickers CE
  TITLE     Coupling gene regulatory patterns to bioprocess conditions to 
            optimize synthetic metabolic modules for improved sesquiterpene 
            production in yeast.
  JOURNAL   Biotechnol Biofuels. 2017 Feb 21;10:43. doi: 
            10.1186/s13068-017-0728-x.
  PUBMED    28239415
REFERENCE   2  (bases 1 to 12779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Biofuels."; date: "2017-02-21"; volume: "10:43. doi"; pages: " 
            10.1186/s13068-017-0728-x"
COMMENT     Sequence Label: pJT9R
FEATURES             Location/Qualifiers
     source          1..12779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(796..843)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     1216..1234
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1272..1294)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1394..1413
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(1907..3001)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(3002..3409)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(3710..4165)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3847..3868)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4059..4078
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4291..4313
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4305..4322
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4306..4322
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4332..4351
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4332..4350
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5763..6204)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(5769..5792)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    6087..6204
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(10420..10440)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10420..10438)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10459..10475)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10459..10475)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10472..10494)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10483..10499
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10507..10513,10514..10531,10532..10537))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10552..10573
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10690..10707)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10861..11449)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10941..10960)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11620..12411,12412..12480))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12243..12262
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12481..12585)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(12612..12779,1..1175)
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca
       61 acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc tacaaaaatg
      121 catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt ctcctttgtg
      181 cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa ggttagaaga
      241 aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt
      301 tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc cccgattata
      361 ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg ttgatgattc
      421 ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata ctacgtatag
      481 gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct tactacaatt
      541 tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc gagtttagat
      601 gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc acagagatat
      661 atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat attttagtag
      721 ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt
      781 tttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggaac
      841 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag
      901 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag
      961 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat
     1021 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt
     1081 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca
     1141 tccttcaatg ctatcatttc ctttgatatt ggatcatact aagaaaccat tattatcatg
     1201 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     1261 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     1321 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     1381 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat cgactacgtc
     1441 gttaaggccg tttctgacag agtaaaattc ttgagggaac tttcaccatt atgggaaatg
     1501 gttcaagaag gtattgactt aaactccatc aaatggtcag gtcattgagt gttttttatt
     1561 tgttgtattt tttttttttt agagaaaatc ctccaatata taaattagga atcatagttt
     1621 catgattttc tgttacacct aactttttgt gtggtgccct cctccttgtc aatattaatg
     1681 ttaaagtgca attctttttc cttatcacgt tgagccatta gtatcaattt gcttacctgt
     1741 attcctttac atcctccttt ttctccttct tgataaatgt atgtagattg cgtatatagt
     1801 ttcgtctacc ctatgaacat attccatttt gtaatttcgt gtcgtttcta ttatgaattt
     1861 catttataaa gtttatgtac aaatatcata aaaaaagaga atctttttaa gcaaggattt
     1921 tcttaacttc ttcggcgaca gcatcaccga cttcggtggt actgttggaa ccacctaaat
     1981 caccagttct gatacctgca tccaaaacct ttttaactgc atcttcaatg gccttacctt
     2041 cttcaggcaa gttcaatgac aatttcaaca tcattgcagc agacaagata gtggcgatag
     2101 ggttgacctt attctttggc aaatctggag cagaaccgtg gcatggttcg tacaaaccaa
     2161 atgcggtgtt cttgtctggc aaagaggcca aggacgcaga tggcaacaaa cccaaggaac
     2221 ctgggataac ggaggcttca tcggagatga tatcaccaaa catgttgctg gtgattataa
     2281 taccatttag gtgggttggg ttcttaacta ggatcatggc ggcagaatca atcaattgat
     2341 gttgaacctt caatgtaggg aattcgttct tgatggtttc ctccacagtt tttctccata
     2401 atcttgaaga ggccaaaaca ttagctttat ccaaggacca aataggcaat ggtggctcat
     2461 gttgtagggc catgaaagcg gccattcttg tgattctttg cacttctgga acggtgtatt
     2521 gttcactatc ccaagcgaca ccatcaccat cgtcttcctt tctcttacca aagtaaatac
     2581 ctcccactaa ttctctgaca acaacgaagt cagtaccttt agcaaattgt ggcttgattg
     2641 gagataagtc taaaagagag tcggatgcaa agttacatgg tcttaagttg gcgtacaatt
     2701 gaagttcttt acggattttt agtaaacctt gttcaggtct aacactaccg gtaccccatt
     2761 taggaccacc cacagcacct aacaaaacgg catcagcctt cttggaggct tccagcgcct
     2821 catctggaag tggaacacct gtagcatcga tagcagcacc accaattaaa tgattttcga
     2881 aatcgaactt gacattggaa cgaacatcag aaatagcttt aagaacctta atggcttcgg
     2941 ctgtgatttc ttgaccaacg tggtcacctg gcaaaacgac gatcttctta ggggcagaca
     3001 ttagaatggt atatccttga aatatatata tatatattgc tgaaatgtaa aaggtaagaa
     3061 aagttagaaa gtaagacgat tgctaaccac ctattggaaa aaacaatagg tccttaaata
     3121 atattgtcaa cttcaagtat tgtgatgcaa gcatttagtc atgaacgctt ctctattcta
     3181 tatgaaaagc cggttccggc gctctcacct ttcctttttc tcccaatttt tcagttgaaa
     3241 aaggtatatg cgtcaggcga cctctgaaat taacaaaaaa tttccagtca tcgaatttga
     3301 ttctgtgcga tagcgcccct gtgtgttctc gttatgttga ggaaaaaaat aatggttgct
     3361 aagagattcg aactcttgca tcttacgata cctgagtatt cccacagtta actgcggtca
     3421 agatatttct tgaatcaggc gccttagacc gctcggccaa acaaccaatt acttgttgag
     3481 aaatagagta taattatcct ataaatataa cgtttttgaa cacacatgaa caaggaagta
     3541 caggacaatt gattttgaag agaatgtgga ttttgatgta attgttggga ttccattttt
     3601 aataaggcaa taatattagg tatgtagata tactagaagt tctcctcgac cggtcgatat
     3661 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaagc
     3721 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     3781 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     3841 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     3901 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     3961 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     4021 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     4081 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     4141 cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag
     4201 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     4261 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     4321 gtgagcgcgc gtaatacgac tcactatagg gcgaattggg taccgggccc attgtagcaa
     4381 agattgtaag gaaatagcaa accagaggtc caagtcaaaa cggcaaaaaa gaagaaagta
     4441 agcaatataa tggacgggtt tacatgttta aatatatgta catgtatgta gtcttatact
     4501 ttcaaaacat aatcgcatgc gggtcatcaa aataaacctc cattttggac ttgggaaaaa
     4561 gaaaatttga tttaaactgt tgtatgtagc taacaataca taaaactatt taaataaaat
     4621 aggaataaga atgctcaatt aaaaaatgat ttattttcta acaaaacttc ctgcaggtca
     4681 ctattatttg cttctcttgt aaactttgtt caaaaacgca gttaagacgt cggctttgaa
     4741 gccacgagac tcgtcgactt gggagatctt ggccttcaaa tccttggcaa cagactcttc
     4801 atattcgtgg tataactggt cgattttcaa gtcattgaaa atctttttgc atttggcttc
     4861 tgcgactgag tccttcttac cgtaattttc gtctaaagtc tttctttgtt ctgcggaagc
     4921 aagttctaat gccttgttga ttacccaaga acatttgtta tcttggatat ctgtaccgat
     4981 cttaccgatc tgttctgggg taccgaagca gtctaagtag tcatcttgaa tttggaaata
     5041 ttcacccaat ggaatcaaga catctctggc ttgtttcaaa tccttttcat ctgtgatacc
     5101 ggcaacgtac atagccaatg cgacaggcaa gtagaaagaa tagtaagcag tcttgaaagt
     5161 aactatgaag gagtgcttct ttagggagaa cttactcaag tcgactttgt cttcaggtgc
     5221 agtgattaag tccatcaatt ggcccaattc ggtttggaag gtgacttcat ggaacaattc
     5281 ggtgatatct atgtagtatt tttcgtttct gaagtgagat ttcaaaagct tgtagatagc
     5341 agcctctaac atgaatgcgt cattgatggc aatttcccca acttcaggaa ccttgtacca
     5401 acatggttgg cctcttctgg taatggactt gtccatcata tcatcggcga ccaagaagta
     5461 agcctgcaac aactcaatgc accaacctag aatagcaacc ttttcgtatt cttcttgccc
     5521 caattgttca acggtcttgt tggagagaat agcatacgtg tccacaacgg acaaacctct
     5581 atttaactta ccgcctggag tgttgtagtt caatgagtgg gcataccagt cacatgcttc
     5641 cttaggcata ccgtaagcca aaagcgatgc gttcaattcc tctactaatt tagggaaaac
     5701 gttcaagaat ctctctctcc taatttcttt ttctgaagcc attttgctag ctatagtttt
     5761 ttctccttga cgttaaagta tagaggtata ttaacaattt tttgttgata cttttatgac
     5821 atttgaataa gaagtaatac aaaccgaaaa tgttgaaagt attagttaaa gtggttatgc
     5881 agcttttgca tttatatatc tgttaataga tcaaaaatca tcgcttcgct gattaattac
     5941 cccagaaata aggctaaaaa actaatcgca ttattatcct atggttgtta atttgattcg
     6001 ttgatttgaa ggtttgtggg gccaggttac tgccaatttt tcctcttcat aaccataaaa
     6061 gctagtattg tagaatcttt attgttcgga gcagtgcggc gcgaggcaca tctgcgtttc
     6121 aggaacgcga ccggtgaaga ccaggacgca cggaggagag tcttccgtcg gagggctgtc
     6181 gcccgctcgg cggcttctaa tccgtacttc aatatagcaa tgagcagtta agcgtattac
     6241 tgaaagttcc aaagagaagg tttttttagg ctaagataat ggggctcttt acatttccac
     6301 aacatataag taagattaga tatggatatg tatatggtgg tattgccatg taatatgatt
     6361 attaaacttc tttgcgtcca tccaaaaaaa aagtaagaat ttttgaaaat tcaatataaa
     6421 ctagtaaagc cctatacttt gcttctttat gtaagaaggt tggtattcca gctggtgtcg
     6481 tcaacatcgt tccaggtcct ggtagaactg ttggtgctgc tttgaccaac gacccaagaa
     6541 tcagaaagct ggcttttacc ggttctacag aagtcggtaa gagtgttgct gtcgactctt
     6601 ctgaatctaa cttgaagaaa atcactttgg aactaggtgg taagtccgcc catttggtct
     6661 ttgacgatgc taacattaag aagactttac caaatctagt aaacggtatt ttcaagaacg
     6721 ctggtcaaat ttgttcctct ggttctagaa tttacgttca agaaggtatt tacgacgaac
     6781 tattggctgc tttcaaggct tacttggaaa ccgaaatcaa agttggtaat ccatttgaca
     6841 aggctaactt ccaaggtgct atcactaacc gtcaacaatt cgacacaatt atgaactaca
     6901 tcgatatcgg taagaaagaa ggcgccaaga tcttaactgg tggcgaaaaa gttggtgaca
     6961 agggttactt catcagacca accgttttct acgatgttaa tgaagacatg agaattgtta
     7021 aggaagaaat ttttggacca gttgtcactg tcgcaaagtt caagacttta gaagaaggtg
     7081 tcgaaatggc taacagctct gaattcggtc taggttctgg tatcgaaaca gaatctttga
     7141 gcacaggttt gaaggtggcc aagatgttga aggccggtac cgtctggatc aacacataca
     7201 acgattttga ctccagagtt ccattcggtg gtgttaagca atctggttac ggtagagaaa
     7261 tgggtgaaga agtctaccat gcatacactg aagtaaaagc tgtcagaatt aagttgtaat
     7321 gtaccaacct gcatttcttt ccgtcatata cacaaaatac tttcatataa acttacttgg
     7381 tcttacgtca taaataaata tgtatacata taaattaaaa aatttggttt tatattttta
     7441 caaaaagaat cgtttacttc atttctccct tttaagcgat acaatccatg aaaaaagaga
     7501 aaaagagaga acaggcttgt gccttcttta aaacatccca cacaaaatca tattgaattg
     7561 aattttacat cttaagctag tgtacaacaa ctgctatatc caaagaaaat aacctcgagt
     7621 gtactaatcc aaggaggttt acggaccaga ggaactttcc agattcagat cacagcaata
     7681 taggactgga aaacatcagg tagccgcact caacttgtaa ctggcaacta ctttgcatta
     7741 aactccaatt aaatgcggta gaatcttttc agaaaaggta ttcaacgtca attcggaaag
     7801 cttccttccg gaatggctta agtaggttgc aatttctttt tctattagta gctaaaaatg
     7861 ggtcacgtga tctatattcg aaaggggcgg ttgcctcagg aaggcaccgg cggtctttcg
     7921 tccgtgcgga gatatctgcg ccgttcaggg gtccatgtgc cttggacgat attaaggcag
     7981 aaggcagtat cggggcggat cactccgaac cgagattagt taagcccttc ccatctcaag
     8041 atggggagca aatggcatta tactcctgct agaaagttaa ctgtgcacat attcttaaat
     8101 tatacaatgt tctggagagc tattgtttaa aaaacaaaca tttcgcaggc taaaatgtgg
     8161 agataggatt agttttgtag acatatataa acaatcagta attggattga aaatttggtg
     8221 ttgtgaattg ctcttcatta tgcaccttat tcaattatca tcaagaatag caatagttaa
     8281 gtaaacacaa gattaacata ataaaaaaaa taattctttc ataggatcca aaatggctac
     8341 cgcagcaggt cctatcgcaa ctaacaactc cccacaaaac tccaacgctt acagaactcc
     8401 aatcgctcct tccgtaccaa ttactcataa atggtctata gctgaagatt tgacatgtat
     8461 ttccaatcct agtaagcaca ataaccctca aactggttac agatcatttt ctgacgaatt
     8521 atacgttaag tacgaagaaa agttggaaga tgttagaaaa gcattaagag aagttgaaga
     8581 aaaccctttg gaaggtttag ttatgataga cgctttgcaa agattgggta tcgattacca
     8641 tttcagaggt gaaattggtg cattcttgca aaagcaacaa atcatatctt caactccaga
     8701 tggttaccct gaacatggtt tgtacgaagt ttcaacattg tttagattct taagacaaga
     8761 aggtcacaat gttaccgctg acgtctttaa taacttcaag gataaggaag gtagattcag
     8821 atcagaattg tcaacagata ttagaggttt gatgtcctta tacgaagcaa gtcaattgag
     8881 aatagaaggt gaagacatct tagatcaagc tgctgatttc tccagtcaat tgttaggtag
     8941 atggacaaaa gatcctaatc atcacgaagc cagattggtt tctaacactt taacacatcc
     9001 ataccacaag tcattggcta cctttatggg tcaaaaattg tcctacatga actgcaaggg
     9061 tccaaactgg gacggtgtcg ataatttgca agaattagct aagatggatt tgactatcgt
     9121 acaaagtatc catcaaaagg aagtattcca agtttctcaa tggtggaagg atacaggttt
     9181 agccaatgaa ttgaaattgg ctagaaacca accattgaag tggtatatgt ggcctatggc
     9241 cgctttaacc gatccaagat tcagtgaaga aagagttgaa ttaactaagc ctatttcttt
     9301 tatatacatc atagatgaca tcttcgacgt ctatggtacc attgaagaat tgaccttgtt
     9361 tactgatgca gttaatagat gggaattgtc tgccgtcgaa caattaccag actacatgaa
     9421 agtatgtttc aaggctttgt acgatgttac caacgaaatc gcatacaaaa tctataaaaa
     9481 gcatggtcaa aaccctattg attccttgca aaagacttgg gctagtttgt gcaatgcatt
     9541 tttagttgaa gcaaagtggt tcgcctctgg tcacttgcca aatgcagaag aatacttaaa
     9601 gaacggtatc atctcttcag gtgttcatgt tgtcttggcc cacatgtttt tcttgttagg
     9661 tgacggtatt acacaagaat cagttgattt ggtagatgac tatccaggta tttccacaag
     9721 tatcgcaacc attttgagat tatctgatga cttgggttca gccaaagatg aagaccaaga
     9781 tggttatgat ggttcttaca tcgaatgtta catgaaggaa cataagggtt ccagtgtcga
     9841 ttcagccaga gaagaagtaa taagaatgat ctccgaagca tggaaatgtt tgaataagga
     9901 atgcttatca ccaaaccctt tttctgaatc attcagaata ggttccttga atatggctag
     9961 aatgatccct atgatgtact cttacgatga caaccataac ttgccaattt tagaagaaca
    10021 catgaaggca atgatctata acacatcttt ataatagtga ccatggcgga ttgagagcaa
    10081 atcgttaagt tcaggtcaag taaaaattga tttcgaaaac taatttctct tatacaatcc
    10141 tttgattgga ccgtcatcct ttcgaatata agattttgtt aagaatattt tagacagaga
    10201 tctactttat atttaatatc tagatattac ataatttcct ctctaataaa atatcattaa
    10261 taaaataaaa atgaagcgat ttgattttgt gttgtcaact tagtttgccg ctatgcctct
    10321 tgggtaatgc tattattgaa tcgaagggct ttattatatt accctttagc ttattctgag
    10381 gtttctgtgg cgtccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc
    10441 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    10501 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    10561 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    10621 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    10681 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    10741 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    10801 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    10861 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    10921 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    10981 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    11041 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    11101 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    11161 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    11221 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    11281 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    11341 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    11401 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    11461 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    11521 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    11581 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    11641 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    11701 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    11761 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    11821 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    11881 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    11941 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    12001 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    12061 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    12121 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    12181 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    12241 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    12301 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    12361 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    12421 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    12481 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    12541 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    12601 agtgccacct gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag
    12661 cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga
    12721 aagcgctatt ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacg
//
