LOCUS       sequence-190504-       11074 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Inducible lentiviral construct to express FLAG-tagged UPF1wt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11074)
  AUTHORS   Feng Q, Jagannathan S, Bradley RK
  TITLE     The RNA Surveillance Factor UPF1 Represses Myogenesis via Its E3 
            Ubiquitin Ligase Activity.
  JOURNAL   Mol Cell. 2017 Jul 20;67(2):239-251.e6. doi: 
            10.1016/j.molcel.2017.05.034. Epub 2017 Jun 29.
  PUBMED    28669802
REFERENCE   2  (bases 1 to 11074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2017.05.034"; journalName: "Mol Cell"; date: 
            "2017-07-20- 20"; volume: "67"; issue: "2"; pages: "239-251.e6"
COMMENT     Sequence Label: pCW57.1-Tet-UPF1WT (pRKB264)
FEATURES             Location/Qualifiers
     source          1..11074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             549..572
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        4016..4526
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4445..4463
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4536..5132
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4536..4555)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5032..5052
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5133..5186
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5187..5804,5805..5810,5811..5846,5847..5849,5850..5885,
                     5886..5888,5889..5924,5925..5933)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5973..5993)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6391..6402)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6607..6840
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6912..7033
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6949..6968)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7003..7022
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7073..7208
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7208..7279
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7280..7348,7349..8140)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7498..7517)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8311..8899
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8800..8819
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9053..9070
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9187..9208
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9223..9228,9229..9246,9247..9253)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9261..9277
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9266..9288
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9285..9301
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9285..9301
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9320..9340
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9322..9340
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9368..9594
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9595..9775
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9822..9947
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10440..10673
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10858..10902
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattcggag
      541 gcaccatgga ctacaaagac gatgacgaca agggcggctc tgtggaagcc tatgggccga
      601 gcagccaaac tctgaccttc ctcgatactg aggaagctga actcctggga gccgacacac
      661 aaggaagcga atttgagttt actgacttta cgctgccctc acagacacag accccaccag
      721 gtggtcctgg aggccccggg ggtggcggcg ccgggggacc cggcggagcc ggtgctgggg
      781 ccgcggcagg ccaactggat gctcaggtag gacctgaagg gatcctccaa aacggagctg
      841 tcgatgacag cgtggctaaa acgagtcagc tcctggccga acttaatttc gaagaagacg
      901 aagaggacac gtattacacc aaggacctgc ctatacacgc ttgcagttac tgcggtatac
      961 acgatcctgc ctgcgtggtt tactgtaata ccagcaagaa gtggttctgc aacggacgtg
     1021 gaaatacttc tggcagccac attgtaaatc accttgtgag ggcaaaatgc aaagaggtga
     1081 ccctgcacaa ggacgggccc ctgggggaga cagtcctgga gtgctacaac tgcggctgtc
     1141 gcaacgtctt cctcctcggc ttcatcccgg ccaaagctga ctcagtggtg gtgctgctgt
     1201 gcaggcagcc ctgtgccagc cagagcagcc tcaaggacat caactgggac agctcgcagt
     1261 ggcagccgct gatccaggac cgctgcttcc tgtcctggct ggtcaagatc ccctccgagc
     1321 aggagcagct gcgggcacgc cagatcacgg cacagcagat caacaagctg gaggagctgt
     1381 ggaaggaaaa cccttctgcc acgctggagg acctggagaa gccgggggtg gacgaggagc
     1441 cgcagcatgt cctcctgcgg tacgaggacg cctaccagta ccagaacata ttcgggcccc
     1501 tggtcaagct ggaggccgac tacgacaaga agctgaagga gtcccagact caagataaca
     1561 tcactgtcag gtgggacctg ggccttaaca agaagagaat cgcctacttc actttgccca
     1621 agactgactc tgacatgcgg ctcatgcagg gggatgagat atgcctgcgg tacaaagggg
     1681 accttgcgcc cctgtggaaa gggatcggcc acgtcatcaa ggtccctgat aattatggcg
     1741 atgagatcgc cattgagctg cggagcagcg tgggtgcacc tgtggaggtg actcacaact
     1801 tccaggtgga ttttgtgtgg aagtcgacct cctttgacag gatgcagagc gcattgaaaa
     1861 cgtttgccgt ggatgagacc tcggtgtctg gctacatcta ccacaagctg ttgggccacg
     1921 aggtggagga cgtaatcatc aagtgccagc tgcccaagcg cttcacggcg cagggcctcc
     1981 ccgacctcaa ccactcccag gtttatgccg tgaagactgt gctgcaaaga ccactgagcc
     2041 tgatccaggg cccgccaggc acggggaaga cggtgacgtc ggccaccatc gtctaccacc
     2101 tggcccggca aggcaacggg ccggtgctgg tgtgtgctcc gagcaacatc gccgtggacc
     2161 agctaacgga gaagatccac cagacggggc taaaggtcgt gcgcctctgc gccaagagcc
     2221 gtgaggccat cgactccccg gtgtcttttc tggccctgca caaccagatc aggaacatgg
     2281 acagcatgcc tgagctgcag aagctgcagc agctgaaaga cgagactggg gagctgtcgt
     2341 ctgccgacga gaagcggtac cgggccttga agcgcaccgc agagagagag ctgctgatga
     2401 acgcagatgt catctgctgc acatgtgtgg gcgccggtga cccgaggctg gccaagatgc
     2461 agttccgctc cattttaatc gacgaaagca cccaggccac cgagccggag tgcatggttc
     2521 ccgtggtcct cggggccaag cagctgatcc ttgtaggcga ccactgccag ctgggcccag
     2581 tggtgatgtg caagaaggcg gccaaggccg ggctgtcaca gtcgctcttc gagcgcctgg
     2641 tggtgctggg catccggccc atccgcctgc aggtccagta ccggatgcac cctgcactca
     2701 gcgccttccc atccaacatc ttctacgagg gctccctcca gaatggtgtc actgcagcgg
     2761 atcgtgtgaa gaagggattt gacttccagt ggccccaacc cgataaaccg atgttcttct
     2821 acgtgaccca gggccaagag gagattgcca gctcgggcac ctcctacctg aacaggaccg
     2881 aggctgcgaa cgtggagaag atcaccacga agttgctgaa ggcaggcgcc aagccggacc
     2941 agattggcat catcacgccc tacgagggcc agcgctccta cctggtgcag tacatgcagt
     3001 tcagcggctc cctgcacacc aagctctacc aggaggtgga gatcgccagt gtggacgcct
     3061 ttcagggacg cgagaaggac ttcatcatcc tgtcctgtgt gcgggccaac gagcaccaag
     3121 gcattggctt tttaaatgac cccaggcgtc tgaacgtggc cctgaccaga gcaaggtatg
     3181 gcgtcatcat tgtgggcaac ccgaaggcac tatcaaagca gccgctctgg aaccacctgc
     3241 tgaactacta taaggagcag aaggtgctgg tggaggggcc gctcaacaac ctgcgtgaga
     3301 gcctcatgca gttcagcaag ccacggaagc tggtcaacac tatcaacccg ggagcccgct
     3361 tcatgaccac agccatgtat gatgcccggg aggccatcat cccaggctcc gtctatgatc
     3421 ggagcagcca gggccggcct tccagcatgt acttccagac ccatgaccag attggcatga
     3481 tcagtgccgg ccctagccac gtggctgcca tgaacattcc catccccttc aacctggtca
     3541 tgccacccat gccaccgcct ggctattttg gacaagccaa cgggcctgct gcagggcgag
     3601 gcaccccgaa aggcaagact ggtcgtgggg gacgccagaa gaaccgcttt gggcttcctg
     3661 gacccagcca gactaacctc cccaacagcc aagccagcca ggatgtggcg tcacagccct
     3721 tctctcaggg cgccctgacg cagggctaca tctccatgag ccagccttcc cagatgagcc
     3781 agcccggcct ctcccagccg gagctgtccc aggacagtta ccttggtgac gagtttaaat
     3841 cacaaatcga cgtggcgctc tcacaggact ccacgtacca gggagagcgg gcttaccagc
     3901 atggcggggt gacggggctg tcccagtatt aaaaggtggc ggcggaagag ctaagcaacg
     3961 tggcccctga acctgaaaca taaaatgaat gcaattgttg ttgttggatc gatccggggt
     4021 tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc
     4081 gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc
     4141 gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc
     4201 cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag
     4261 ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc
     4321 gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc
     4381 gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg
     4441 cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg
     4501 accgaatcac cgacctctct ccccagcaat tcaccatgac cgagtacaag cccacggtgc
     4561 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     4621 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     4681 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     4741 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     4801 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     4861 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     4921 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     4981 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     5041 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     5101 gcacctggtg catgacccgc aagcccggtg ccgaaggtag aggttctctc ctcacttgtg
     5161 gtgatgttga agaaaaccct ggtccaatgt ctagactgga caagagcaaa gtcataaacg
     5221 gagctctgga attactcaat ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc
     5281 aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc
     5341 tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag
     5401 gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc ataccgctgt gctctcctct
     5461 cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa
     5521 ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt
     5581 acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc
     5641 aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac
     5701 aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac
     5761 taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg accgacgccc
     5821 ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt gaccttgata
     5881 tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg taactaagta
     5941 aggatcgatc caagatatcg tattcttaac tatgttgctc cttttacgct atgtggatac
     6001 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     6061 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     6121 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     6181 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     6241 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     6301 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     6361 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     6421 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     6481 cggatctccc tttgggccgc ctccccgcct gtttcgcctc ggcgtccgga ctagaggtac
     6541 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     6601 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     6661 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6721 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6781 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6841 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     6901 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6961 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7021 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     7081 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7141 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7201 tggaggcctt tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     7261 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     7321 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     7381 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     7441 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     7501 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     7561 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     7621 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     7681 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     7741 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     7801 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     7861 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     7921 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     7981 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8041 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     8101 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     8161 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     8221 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8281 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8341 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     8401 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     8461 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8521 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8581 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     8641 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8701 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8761 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8821 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     8881 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     8941 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9001 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     9061 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     9121 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     9181 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     9241 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     9301 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
     9361 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
     9421 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
     9481 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
     9541 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
     9601 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     9661 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     9721 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     9781 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     9841 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     9901 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     9961 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
    10021 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
    10081 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
    10141 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
    10201 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
    10261 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
    10321 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
    10381 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
    10441 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
    10501 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
    10561 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
    10621 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
    10681 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
    10741 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
    10801 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
    10861 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
    10921 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
    10981 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
    11041 tcgtttcaga cccacctccc aaccccgagg ggac
//
