LOCUS       sequence-190525-        9349 bp    DNA     circular SYN 14-APR-2025
DEFINITION  GA biosensor expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9349)
  AUTHORS   Rizza A, Walia A, Lanquar V, Frommer WB, Jones AM
  TITLE     In vivo gibberellin gradients visualized in rapidly elongating 
            tissues.
  JOURNAL   Nat Plants. 2017 Oct 2. doi: 10.1038/s41477-017-0021-9.
  PUBMED    28970478
REFERENCE   2  (bases 1 to 9349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2017 Oct 2. doi: 10.1038/s41477-017-0021-9."
COMMENT     Sequence Label: pDR FLIP43 GPS1-NR
FEATURES             Location/Qualifiers
     source          1..9349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             465..487
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             507..524
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             528..560
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(564..572,573..587)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    1302..1326
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2620..2644)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2658..2660,2661..3371)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALFKDPNEKRDHMVLLE
                     FLTAAGITLGMDELYK"
     primer_bind     complement(2700..2721)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2961..2980)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             3372..3401
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3439..3455)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3440..3456)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(3480..3499)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3481..3499)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      3647..3834
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3702..3726)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3846..3863)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3846..3862)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3855..3877)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4298..5644
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(5755..6558)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6559..6774)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6799..6817)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6885..6989
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6990..7058,7059..7850)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7208..7227)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8021..8609
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8510..8529
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8763..8780
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8897..8918
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8933..8938,8939..8956,8957..8963)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8971..8987
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8976..8998
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8995..9011
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8995..9011
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtagaactat gagctttttt tgtttctgtt ttcctttttt ttttttttac ctctgtggaa
       61 attgttactc tcacactctt tagttcgttt gtttgttttg tttattccaa ttatgaccgg
      121 tgacgaaacg tggtcgatgg tgggtaccgc ttatgctccc ctccattagt ttcgattata
      181 taaaaaggcc aaatattgta ttattttcaa atgtcctatc attatcgtct aacatctaat
      241 ttctcttaaa ttttttctct ttctttccta taacaccaat agtgaaaatc tttttttctt
      301 ctatatctac aaaaactttt tttttctatc aacctcgttg ataaattttt tctttaacaa
      361 tcgttaataa ttaattaatt ggaaaataac cattttttct ctcttttata cacacattca
      421 aaagaaagaa aaaaaatata ccccagcctc gatctagaaa taattttgtt taactttaag
      481 aaggagatat acatatgcgg ggttctcatc atcatcatca tcatggtatg gctagcatga
      541 ctggtggaca gcaaatgggt cgggatctgt acgacgatga cgataaggat ccgggcctcg
      601 aggtttctaa aggtgaagaa ttgtttacgg gcgtcgtccc gatcctcgtg gaactcgacg
      661 gggatgttaa cgggcataag ttttcggtca gcggggaagg ggagggggac gcgacgtatg
      721 ggaagctcac tctcaagctg atctgtacga cggggaaact cccggtcccg tggccgacgc
      781 tggtcacgac gctgggatac gggctccaat gctttgcgag gtatccggac cacatgaaac
      841 agcatgactt tttcaaatcg gcgatgccgg agggatacgt gcaggaacgg acgatctttt
      901 tcaaagacga tgggaactat aagacgcggg cggaagtcaa gtttgaaggg gacacgctcg
      961 tcaaccggat cgaactcaag gggattgact tcaaagagga tgggaacata ctcggccata
     1021 agctcgaata caattacaac tcgcataacg tatacatcac cgcggataag caaaagaacg
     1081 ggatcaaagc caatttcaaa atccggcata acatagagga tgggggggtc caactggcgg
     1141 atcactatca gcaaaacacg ccgatagggg atgggccggt cctcctcccg gataaccatt
     1201 acctctcgta ccaaagcgcg ctcttcaagg acccgaatga gaaacgggac cacatggttc
     1261 tcctggagtt cctcacggcg gcgggcatag gtaccgatat cacaagtttg tacaaaaaag
     1321 caggcttcga tgagcttcta gctgttcttg gttacaaggt taggtcatcc gaaatggctg
     1381 atgttgctca gaaactcaag cagcttagag ttatgatgtc taatgttcaa gaagacgatc
     1441 tttctcaact cgctactgag actgttcact ataatccggc ggagctttac acgtggcttg
     1501 attctatgct caccgacctt aatcctccgt cgtctaacgc cgagtacgat gcaagtggcg
     1561 cgcctggggc cggccgaagt tctggaatgg ctggaagtga agaagttaat cttattgaga
     1621 gcaagacagt ggttcctctc aatacatggg ttctaatatc caactttaag ctagcttaca
     1681 atctcctgcg tcgccctgac ggaactttta accgtcatct cgcagagttt ctagaccgga
     1741 aagtccctgc aaatgccaac cctgttaatg gggtcttctc ttttgatgtg atcatcgatc
     1801 gccaaactaa tttgcttagc agagtttaca gaccagctga tgctggcact tcaccaagta
     1861 ttactgatct acagaatcct gttgatggtg aaatagtgcc tgttattgtc ttctttcatg
     1921 gtggagccgc tgcacattct tctgcaaaca gtgctattta tgatactctt tgtcgtaggc
     1981 ttgttggttt gtgtggtgct gttgttgtct ctgtgaatta tcgtcgtgca ccagagaatc
     2041 gatacccttg tgcttatgat gatggatggg ctgttttgaa atgggtcaac tcgagttctt
     2101 ggcttagaag caagaaagac tcaaaggttc gtattttctt ggcgggtgat agctctgggg
     2161 gtaacattgt gcataatgtc gcggtaagag cggttgagtc aaggatcgat gttttgggga
     2221 acattttgct taaccctatg tttggaggga ccgaaagaac ggaatctgag aaacgtttgg
     2281 atgggaagta ctttgttacg gttagagacc gagattggta ttggagagcg tttcttcctg
     2341 agggtgaaga cagagagcat ccagcgtgta gcccgtttgg cccgagaagc aagagtctag
     2401 aagggttgag tttccccaag agtcttgtcg ttgtagcggg tttagatttg attcaagatt
     2461 ggcaattgaa gtacgcggaa gggctcaaga aagcgggtca agaggtgaag cttctttact
     2521 tggagcaagc cactattggc ttctacttat tgcctaacaa caatcacttc cataccgtta
     2581 tggatgagat agctgcattt gtaaacgcag aatgccaaca cccagctttc ttgtacaaag
     2641 tggtgatatc gactagtgtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     2701 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     2761 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggt aagctgcccg
     2821 tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc gcccgctacc
     2881 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     2941 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     3001 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     3061 acatcctggg gcacaagctg gagtacaacg ccatcagcga caacgtctat atcaccgccg
     3121 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggca
     3181 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     3241 tgcccgacaa ccactacctg agcacccagt ccgccctgtt caaagacccc aacgagaagc
     3301 gcgatcacat ggtcctgctg gagttcctga ccgccgccgg gatcactctc ggcatggacg
     3361 agctgtacaa ggaacaaaaa ttgataagtg aggaagattt ataagggccg ggctgcagga
     3421 attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta cccaattcgc
     3481 cctatagtga gtcgtattac gcgcggatcc agctttggac ttcttcgcca gaggtttggt
     3541 caagtctcca atcaaggttg tcggcttgtc taccttgcca gaaatttacg aaaagatgga
     3601 aaagggtcaa atcgttggta gatacgttgt tgacacttct aaataagcga atttcttatg
     3661 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag
     3721 tgactcttag gttttaaaac garaattctt attcttgagt aactctttcc tgtaggtcag
     3781 gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta ccggcatgcc
     3841 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     3901 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     3961 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
     4021 ctccttacgc atctgtgcgg tatttcacac cgcataatcg gatcgtactt gttacccatc
     4081 attgaatttt gaacatccga acctgggagt tttccctgaa acagatagta tatttgaacc
     4141 tgtataataa tatatagtct agcgctttac ggaagacaat gtatgtattt cggttcctgg
     4201 agaaactatt gcatctattg cataggtaat cttgcacgtc gcatccccgg ttcattttct
     4261 gcgtttccat cttgcacttc aatagcatat ctttgttaac gaagcatctg tgcttcattt
     4321 tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat
     4381 ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc
     4441 atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc
     4501 tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat
     4561 acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct
     4621 tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca
     4681 ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa
     4741 aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt
     4801 caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa
     4861 cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta
     4921 ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac
     4981 tctatgaata gttcttacta caattttttt gtctaaagag taatactaga gataaacata
     5041 aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt
     5101 atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga
     5161 agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa
     5221 agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag
     5281 ctagagaata ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg agcgcttccg
     5341 aaaatgcaac gcgagctgcg cacatacagc tcactgttca cgtcgcacct atatctgcgt
     5401 gttgcctgta tatatatata catgagaaga acggcatagt gcgtgtttat gcttaaatgc
     5461 gtacttatat gcgtctattt atgtaggatg aaaggtagtc tagtacctcc tgtgatatta
     5521 tcccattcca tgcggggtat cgtatgcttc cttcagcact accctttagc tgttctatat
     5581 gctgccactc ctcaattgga ttagtctcat ccttcaatgc tatcatttcc tttgatattg
     5641 gatcgatccg atgataagct gtcaaacatg agaattgggt aataactgat ataattaaat
     5701 tgaagctcta atttgtgagt ttagtataca tgcatttact tataatacag ttttttagtt
     5761 ttgctggccg catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc
     5821 taccttagca tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc
     5881 tgtagagacc acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc
     5941 taaacccaca ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc
     6001 tctttgagca ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc
     6061 cttagtatat tctccagtag atagggagcc cttgcatgac aattctgcta acatcaaaag
     6121 gcctctaggt tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa caatacctgg
     6181 gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc
     6241 agagtactgc aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa
     6301 aaaattgtac ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc
     6361 agtcaagata tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa
     6421 ctccagtaat tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc
     6481 gtgcatgata ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt
     6541 atatgtagct ttcgacatga tttatcttcg tttcctgcat gtttttgttc tgtgcagttg
     6601 ggttaagaat actgggcaat ttcatgtttc ttcaacacta catatgcgta tatataccaa
     6661 tctaagtctg tgctccttcc ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa
     6721 atttcaagga aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat tgaaaagcta
     6781 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     6841 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6901 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6961 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7021 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7081 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7141 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7201 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7261 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7321 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7381 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7441 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7501 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7561 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7621 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7681 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7741 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7801 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7861 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7921 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7981 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8041 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8101 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8161 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8221 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8281 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8341 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8401 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8461 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8521 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8581 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8641 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8701 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8761 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8821 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     8881 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     8941 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     9001 acagctatga ccatgattac gccaagcttc ctgaaacgga gaaacataaa caggcattgc
     9061 tgggatcacc catacatcac tctgttttgc ctgacctttt ccggtaattt gaaaacaaac
     9121 ccggtctcga agcggagatc cggcgataat taccgcagaa ataaacccat acacgagacg
     9181 tagaaccagc cgcacatggc cggagaaact cctgcgagaa tttcgtaaac tcgcgcgcat
     9241 tgcatctgta tttcctaatg cggcacttcc aggcctcgat cgagaccgtt tatccattgc
     9301 ttttttgttg tctttttccc tcgttcacag aaagtctgaa gaagctata
//
