LOCUS       sequence-190667-         699 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Encodes dCas9(C)-VP64 with SAM components fused to transmembrane 
            tether.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Baeumler TA, Ahmed AA, Fulga TA
  TITLE     Engineering Synthetic Signaling Pathways with Programmable 
            dCas9-Based Chimeric Receptors.
  JOURNAL   Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 
            10.1016/j.celrep.2017.08.044.
  PUBMED    28903044
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-12- 12"; volume: "20"; issue: "11"; pages: "2639-2653"
COMMENT     Sequence Label: pTMt_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65...
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             210..581
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     primer_bind     complement(609..626)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ccgaaccaat gctgatggag taccccgaag ccattacccg gctggtgacc ggcagccagc
       61 ggccccccga ccccgctcca actcccctgg gaaccagcgg cctgcctaat gggctgtccg
      121 gagatgaaga cttctcaagc atcgctgata tggactttag tgccctgctg tcacagattt
      181 cctctagtgg gcagggagga ggtggaagcg gcttcagcgt ggacaccagt gccctgctgg
      241 acctgttcag cccctcggtg accgtgcccg acatgagcct gcctgacctt gacagcagcc
      301 tggccagtat ccaagagctc ctgtctcccc aggagccccc caggcctccc gaggcagaga
      361 acagcagccc ggattcaggg aagcagctgg tgcactacac agcgcagccg ctgttcctgc
      421 tggaccccgg ctccgtggac accgggagca acgacctgcc ggtgctgttt gagctgggag
      481 agggctccta cttctccgaa ggggacggct tcgccgagga ccccaccatc tccctgctga
      541 caggctcgga gcctcccaaa gccaaggacc ccactgtctc ctaagaattc ctagagctcg
      601 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
      661 gccttccttg accctggaag gtgccactcc cactgtcct
//
