LOCUS       sequence-190714-        6418 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Fluorescent protein for superresolution imaging and a focal adhesion
            protein for binding substrate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Wang S, Moffitt JR, Dempsey GT, Xie XS, Zhuang X
  TITLE     Characterization and development of photoactivatable fluorescent 
            proteins for single-molecule-based superresolution imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):8452-7. doi: 
            10.1073/pnas.1406593111. Epub 2014 May 27.
  PUBMED    24912163
REFERENCE   2  (bases 1 to 6418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1406593111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-06-10- 10"; volume: "111"; issue: "23"; pages: "8452-7"
COMMENT     Sequence Label: pZyxin-mMaple3
FEATURES             Location/Qualifiers
     source          1..6418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      634..643
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2364..2373
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3206..3327
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3243..3262)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3297..3316
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3334..3789)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3471..3492)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3683..3702
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3816..3920
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3922..4279
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3922..4113
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3945..3965)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4130..4265
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4314..5108
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4314..5108
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4368..4387)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4978..4997
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5296..5315)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5340..5387
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5716..6304
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6205..6224
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attccatggc ggccccccgc ccgtctcccg
      661 cgatctccgt ttcggtctcg gctccggctt tttacgcccc gcagaagaag ttcggccctg
      721 tggtggcccc aaagcccaaa gtgaatccct tccggcccgg ggacagcgag cctcccccgg
      781 cacccggggc ccagcgcgca cagatgggcc gggtgggcga gattcccccg ccgcccccgg
      841 aagactttcc cctgcctcca cctccccttg ctggggatgg cgacgatgca gagggtgctc
      901 tgggaggtgc cttcccgccg ccccctcccc cgatcgagga atcatttccc cctgcgcctc
      961 tggaggagga gatcttccct tccccgccgc ctcctccgga ggaggaggga gggcctgagg
     1021 cccccatacc gcccccacca cagcccaggg agaaggtgag cagtattgat ttggagatcg
     1081 actctctgtc ctcactgctg gatgacatga ccaagaatga tcctttcaaa gcccgggtgt
     1141 catctggata tgtgccccca ccagtggcca ctccattcag ttccaagtcc agtaccaagc
     1201 ctgcagccgg gggcacagca cccctgcctc cttggaagtc cccttccagc tcccagcctc
     1261 tgccccaggt tccggctccg gctcagagcc agacacagtt ccatgttcag ccccagcccc
     1321 agcccaagcc tcaggtccaa ctccatgtcc agtcccagac ccagcctgtg tctttggcta
     1381 acacccagcc ccgagggccc ccagcctcat ctccggctcc agcccctaag ttttctccag
     1441 tgactcctaa gtttactcct gtggcttcca agttcagtcc tggagcccca ggtggatctg
     1501 ggtcacaacc aaatcaaaaa ttggggcacc ccgaagctct ttctgctggc acaggctccc
     1561 ctcaacctcc cagcttcacc tatgcccagc agagggagaa gccccgagtg caggagaagc
     1621 agcaccccgt gcccccaccg gctcagaacc aaaaccaggt gcgctcccct ggggccccag
     1681 ggcccctgac tctgaaggag gtggaggagc tggagcagct gacccagcag ctaatgcagg
     1741 acatggagca tcctcagagg cagaatgtgg ctgtcaacga actctgcggc cgatgccatc
     1801 aacccctggc ccgggcgcag ccagccgtcc gcgctctagg gcagctgttc cacatcgcct
     1861 gcttcacctg ccaccagtgt gcgcagcagc tccagggcca gcagttctac agtctggagg
     1921 gggcgccgta ctgcgagggc tgttacactg acaccctgga gaagtgtaac acctgcgggg
     1981 agcccatcac tgaccgcatg ctgagggcca cgggcaaggc ctatcacccg cactgcttca
     2041 cctgtgtggt ctgcgcccgc cccctggagg gcacctcctt catcgtggac caggccaacc
     2101 ggccccactg tgtccccgac taccacaagc agtacgcccc gaggtgctcc gtctgctctg
     2161 agcccatcat gcctgagcct ggccgagatg agactgtgcg agtggtcgcc ctggacaaga
     2221 acttccacat gaagtgttac aagtgtgagg actgcgggaa gcccctgtcg attgaggcag
     2281 atgacaatgg ctgcttcccc ctggacggtc acgtgctctg tcggaagtgc cacactgcta
     2341 gagcccagac ggatccaccg gtcgccacca tggtgagcaa aggcgaggag acaatcatgt
     2401 ccgtgatcaa gcccgacatg aagatcaaac tgaggatgga gggcaacgtg aacggccacg
     2461 ccttcgtgat cgagggcgaa ggaagcggca agcccttcga gggcatccag accatcgatc
     2521 tggaggtcaa ggagggcgct cccctccctt tcgcctatga catcctgacc accgccttcc
     2581 actacggcaa tagggtgttc accaagtatc ccaggaagat ccccgactac ttcaagcaga
     2641 gcttccctga gggctacagc tgggagagga gcatgacata cgaggacggc ggcatctgca
     2701 acgccaccaa cgacatcaca atggaggagg acagcttcat caacaagatc cacttcaaag
     2761 gcacaaactt cccccccaat ggccccgtga tgcagaagag gaccgtgggc tgggaggtga
     2821 gcaccgagaa gatgtacgtg agggacggcg tcctgaaggg cgacgtgaag atgaagctcc
     2881 tgctcaaggg cggcagccac tacaggtgcg actttaggac cacctataag gtgaagcaga
     2941 aggctgtgaa gctgcccaag gcccacttcg tcgaccatag gatcgagatc ctgtcccacg
     3001 acaaggacta caacaaggtc aagctgtacg agcacgccgt cgctaggaac agcaccgaca
     3061 gcatggacga actctataag tgaagcggcc gcgactctag atcataatca gccataccac
     3121 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     3181 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     3241 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3301 tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa
     3361 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     3421 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     3481 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     3541 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     3601 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     3661 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     3721 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     3781 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3841 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3901 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     3961 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4021 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4081 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4141 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4201 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4261 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg
     4321 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     4381 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     4441 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
     4501 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     4561 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     4621 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     4681 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     4741 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     4801 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
     4861 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     4921 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     4981 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     5041 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     5101 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     5161 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     5221 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     5281 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
     5341 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt
     5401 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
     5461 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
     5521 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat
     5581 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5641 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5701 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5761 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5821 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     5881 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5941 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6001 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6061 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6121 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6181 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6241 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6301 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6361 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
